Production of colony-stimulating factor in human dental pulp fibroblasts.

Abstract:

:Class II major histocompatilibity complex (MHC)-expressing cells are usually distributed in dental pulp, and it was postulated that the colony-stimulating factor (CSF) derived from dental pulp fibroblasts contributes to the migration of class II MHC-expressing cells into pulp tissue. This study aimed to investigate the CSF production of human dental pulp fibroblasts. In pulp tissue sections, granulocyte (G)-CSF was detected from normal teeth, while G-CSF, macrophage (M)-CSF, and granulocyte-macrophage (GM)-CSF were detected from teeth with dentinal caries. In cultured dental pulp fibroblasts, G-CSF was detected by immunostaining, immunoprecipitation, and ELISA, and mRNAs of G-CSF, M-CSF, and GM-CSF were detected by RT-PCR. The dental pulp fibroblasts cultured with TNF-alpha were found to increase the G-CSF expression and to produce M-CSF and GM-CSF. These findings suggest that dental pulp fibroblasts usually produce G-CSF. In the presence of TNF-alpha, dental pulp fibroblast express M-CSF and GM-CSF.

journal_name

J Dent Res

authors

Sawa Y,Horie Y,Yamaoka Y,Ebata N,Kim T,Yoshida S

doi

10.1177/154405910308200204

keywords:

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

96-100

issue

2

eissn

0022-0345

issn

1544-0591

journal_volume

82

pub_type

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