SHED differentiate into functional odontoblasts and endothelium.

Abstract:

:Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for the understanding of the biology of odontogenesis and for dental tissue engineering. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts and endothelial cells. SHED were seeded in tooth slice/scaffolds and implanted subcutaneously into immunodeficient mice. SHED differentiated into functional odontoblasts that generated tubular dentin, as determined by tetracycline staining and confocal microscopy. These cells also differentiated into vascular endothelial cells, as determined by beta-galactosidase staining of LacZ-tagged SHED. In vitro, vascular endothelial growth factor (VEGF) induced SHED to express VEGFR2, CD31, and VE-Cadherin (markers of endothelium) and to organize into capillary-like sprouts. VEGF induced ERK and AKT phosphorylation (indicative of differentiation), while inhibiting phosphorylation of STAT3 (indicative of 'stemness'). Collectively, this work demonstrates that SHED can differentiate into angiogenic endothelial cells and odontoblasts capable of generating tubular dentin.

journal_name

J Dent Res

authors

Sakai VT,Zhang Z,Dong Z,Neiva KG,Machado MA,Shi S,Santos CF,Nör JE

doi

10.1177/0022034510368647

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

791-6

issue

8

eissn

0022-0345

issn

1544-0591

pii

0022034510368647

journal_volume

89

pub_type

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