Abstract:
:Studies on mechanisms underlying the differentiation of dental pulp stem cells are critical for the understanding of the biology of odontogenesis and for dental tissue engineering. Here, we tested the hypothesis that stem cells from exfoliated deciduous teeth (SHED) differentiate into functional odontoblasts and endothelial cells. SHED were seeded in tooth slice/scaffolds and implanted subcutaneously into immunodeficient mice. SHED differentiated into functional odontoblasts that generated tubular dentin, as determined by tetracycline staining and confocal microscopy. These cells also differentiated into vascular endothelial cells, as determined by beta-galactosidase staining of LacZ-tagged SHED. In vitro, vascular endothelial growth factor (VEGF) induced SHED to express VEGFR2, CD31, and VE-Cadherin (markers of endothelium) and to organize into capillary-like sprouts. VEGF induced ERK and AKT phosphorylation (indicative of differentiation), while inhibiting phosphorylation of STAT3 (indicative of 'stemness'). Collectively, this work demonstrates that SHED can differentiate into angiogenic endothelial cells and odontoblasts capable of generating tubular dentin.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Sakai VT,Zhang Z,Dong Z,Neiva KG,Machado MA,Shi S,Santos CF,Nör JEdoi
10.1177/0022034510368647subject
Has Abstractpub_date
2010-08-01 00:00:00pages
791-6issue
8eissn
0022-0345issn
1544-0591pii
0022034510368647journal_volume
89pub_type
杂志文章abstract::Selective recruitment of periodontal ligament cells to a previously exposed root surface is believed to enhance periodontal regeneration. It has been hypothesized that competition from gingival fibroblasts may reduce the potential of periodontal regeneration. We compared the migratory responses of PDL cells and gingiv...
journal_title:Journal of dental research
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abstract::Human saliva contains a number of phosphorylated acidic proline-rich proteins (APRP). Monkey parotid saliva contains a similar protein with the same phosphorylated sequences as the human proteins. A crude protein kinase was prepared from Macaca fascicularis parotid glands which phosphorylated human APRP. The enzyme wa...
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abstract::The complexity of the periodontal microbiota resembles that of the gastro-intestinal tract, where infectious diseases are treatable via probiotics. In the oropharyngeal region, probiotic or replacement therapies have shown some benefit in the prevention of dental caries, otitis media, and pharyngitis, but their effect...
journal_title:Journal of dental research
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doi:10.1177/154405910708601111
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abstract::For restoring cavitated dental lesions, whether carious or not, a large number of material combinations are available. We aimed to systematically review and synthesize data of comparative dental restorative trials. A systematic review was performed. Randomized controlled trials published between 2005 and 2015 were inc...
journal_title:Journal of dental research
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abstract::Genetic influences are important in the determination of mandibular morphology, and growth hormone receptor (GHR) is believed to have an important influence on the growth of craniofacial bone. In this study, we used quantitative trait locus methods to evaluate the relationship between craniofacial morphology and singl...
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abstract::The effects of demineralized dentin and bone matrix on dental ectomesenchymal cells were evaluated after observation periods of two or three weeks. Autogenous dentin and bone matrix, obtained from the crowns of primary molars or maxillary cortical bone, respectively, were demineralized with 3% acetic acid and implante...
journal_title:Journal of dental research
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doi:10.1177/00220345870660091401
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journal_title:Journal of dental research
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abstract::The microstructures of (beta + gamma) silver-tin alloys are especially influenced by both homogenization temperature and subsequent heat treatment. When the alloy is cooled from homogenization temperatures above approximately 200 degrees C, lenticular regions of the ordered orthorhombic gamma phase precipitate from wi...
journal_title:Journal of dental research
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doi:10.1177/00220345850640051501
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journal_title:Journal of dental research
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journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345880670090801
更新日期:1988-09-01 00:00:00
abstract::In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a ...
journal_title:Journal of dental research
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doi:10.1177/154405910808700716
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::As a major intracellular degradation and recycling machinery, autophagy plays an important role in maintaining cellular homeostasis and remodeling during normal development. Our previous study showed that fluorapatite (FA) crystal-coated electrospun polycaprolactone (PCL) was capable of inducing differentiation and mi...
journal_title:Journal of dental research
pub_type: 杂志文章
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abstract::During the setting of a resin composite cement (RCC) used as an adhesive between a resin-bonded bridge and tooth structure, the adhesion may be disrupted by the development of shrinkage stress. The aim of this study was to investigate the influence of the shrinkage stress of three different RCCs on their adhesive and ...
journal_title:Journal of dental research
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更新日期:1992-02-01 00:00:00
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journal_title:Journal of dental research
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abstract::In mammals, the periodontal ligament (PDL) is a highly specialized tissue which facilitates tooth eruption and lends mechanical support to the tooth once in occlusion. The PDL extracellular matrix fibers play a major role in such functions. During its development, the spatial arrangement of the PDL extracellular matri...
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