Abstract:
:The structure of three types of liposomes (egg yolk phosphatidylcholine (EPC) without modification and EPC vesicles containing cross-linked N-isopropylacrylamide (NIPAM) networks of low and a high concentration inside the vesicles) were analyzed by static and dynamic light scattering. Upon polymerization the network was assumed to become attached to the membrane by reactive anchoring monomers. For the sample of high poly(NIPAM) content the polymer network was assumed to fill the whole space in the vesicles. The issue of the present study was to examine hard and hollow sphere behavior of the liposomes with networks of high and low poly(NIPAM) content. The theoretical scattering curves differ markedly for uniform hard and uniform hollow spheres by the presence of specific peaks. However, polydispersity washed out the peaks and led to smoothed asymptotes with fractal dimensions of df = 2 for hollow and df = 4 for hard spheres. The experimental data could efficiently be fitted with weakly polydisperse hollow spheres. No clear conclusion could be drawn from the angular dependence alone for the liposome of high poly(NIPAM) content. The two wavelengths from the HeNe and Ar lasers proved to be too long for the studied liposomes of about 100 nm in radius. However, evidence for hollow sphere behavior was found for fractionated liposomes from the ratio rho = Rg/Rh = 1.04 +/- 0.02 (theory rho = 1.00 for hollow spheres). Finally, from the molar mass and the sphere radius, an apparent density was determined. The analysis gave the expected density for the pure EPC lecithin vesicles and a poly(NIPAM) network density of 0.244 g/mL. For the liposome of low poly(NIPAM) content the network appeared to be attached to the inner surface of the lecithin shell to form a layer of about 18 nm thickness.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Stauch O,Schubert R,Savin G,Burchard Wdoi
10.1021/bm0200074keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
565-78issue
3eissn
1525-7797issn
1526-4602pii
bm0200074journal_volume
3pub_type
杂志文章abstract::We investigate the effects of mixing a colloidal suspension of tunicate-derived cellulose nanocrystals (t-CNCs) with aqueous colloidal suspensions of two protein diblock copolymers, EC and CE, which bear two different self-assembling domains (SADs) derived from elastin (E) and the coiled-coil region of cartilage oligo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401304w
更新日期:2013-12-09 00:00:00
abstract::Reductively degradable biopolymers have emerged as a unique class of smart biomedical materials. Here, a functional coupling agent, cystamine diisocyanate (CDI), was designed to offer a facile access to reductively degradable biopolymers via polycondensation with various diols. CDI was readily obtained with a decent y...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01578
更新日期:2016-03-14 00:00:00
abstract::Covalently modified albumin (BSA) microparticles were developed for potential use as an adjuvant in mucosal vaccines against hepatitis B. To synthesize consistent protein particles, a covalent approach was proposed to modify BSA. Our strategy was to bond maleic anhydride (MA) molecules to BSA structure by nucleophilic...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400859z
更新日期:2013-09-09 00:00:00
abstract::The effect of increasing water composition on the rheological and microstructural behavior of a ternary cellulose acetate (CA)/N,N-dimethylacetamide (DMA)/water system is examined. Addition of water to the CA/DMA system results in enhanced steady shear viscosity and dynamic viscoelastic properties and ultimately to ph...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm0611681
更新日期:2007-05-01 00:00:00
abstract::The drying dynamics of protein coatings is of importance for many applications. The main focus of research so far was to investigate macroscopic properties of protein coatings, leaving drying dynamics virtually unexplored. A unique combination of techniques is used to monitor drying of a coating containing the protein...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm501549b
更新日期:2015-01-12 00:00:00
abstract::The detection of phase separation and identification of miscibility in biopolymer blends is an important aspect for the improvement of their physical properties. In this article, the phase separation in blends of poly(3-hydroxybutyrate) (PHB) with poly(L-lactic acid) (PLA) and poly(epsilon-caprolactone) (PCL), respect...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm701035p
更新日期:2008-02-01 00:00:00
abstract::The single and triple helical organization of protein chains strongly influences the mechanical properties of gelatin-based materials. A chemical method for obtaining different degrees of helical organization in gelatin is covalent functionalization, while a physical method for achieving the same goal is the variation...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm101029k
更新日期:2011-01-10 00:00:00
abstract::Nanofibrous materials containing the antitumor drug doxorubicin hydrochloride (DOX) were easily prepared using a one-step method by electrospinning of DOX/poly(L-lactide-co-D,L-lactide) (coPLA) and DOX/quaternized chitosan (QCh)/coPLA solutions. The pristine and DOX-containing mats were characterized by ATR-FTIR and X...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100285n
更新日期:2010-06-14 00:00:00
abstract::The copper(II) complex formation equilibria of N-(2-carboxyethyl)chitosans with three different degrees of substitution (DS = 0.42, 0.92, and 1.61) were studied in aqueous solution by pH-potentiometric and UV-spectrophotometric techniques. It was demonstrated that the complexation model of CE-chitosans depends on DS: ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm049597r
更新日期:2005-01-01 00:00:00
abstract::Nanofibrous biocomposite scaffolds of type I collagen and nanohydroxyapatite (nanoHA) of varying compositions (wt %) were prepared by electrostatic cospinning. The scaffolds were characterized for structure and morphology by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic fo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm060879w
更新日期:2007-02-01 00:00:00
abstract::Biodegradable multilayered capsules encapsulating basic fibroblast growth factor (bFGF) were developed as a cytokine release carrier for drug delivery systems. The multilayered hollow capsules were fabricated via the layer-by-layer (LbL) assembly of chitosan (CT) and dextran sulfate (Dex). The bFGF was encapsulated in...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800321w
更新日期:2008-08-01 00:00:00
abstract::It was aim of the study to investigate the in vivo potential of a novel insulin-thiomer complex nanoparticulate delivery system. Insulin loaded nanoparticles were obtained by the formation of hydrogen bonds between poly(vinyl pyrrolidone) (PVP) and poly(acrylic acid)-cysteine (PAA-Cys) or poly(acrylic acid) (PAA), res...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700916h
更新日期:2008-01-01 00:00:00
abstract::A novel approach for the preparation of nano- and microcapsules in aqueous solutions by using thermoresponsive polymer (TRP) templates (mesoglobules) is described. The method comprised three steps: formation of mesoglobules, coating the templates by seeded radical copolymerization, followed by core dissolution and cor...
journal_title:Biomacromolecules
pub_type: 杂志文章,评审
doi:10.1021/bm501194g
更新日期:2014-12-08 00:00:00
abstract::Several alternatives to the conventional alginate beads formulation were studied for encapsulation of invertase. Pectin was added to the alginate/enzyme solution while trehalose and β-cyclodextrin were added to the calcium gelation media. The effect of composition changes, freezing, drying methods (freeze, vacuum, or ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm2009075
更新日期:2011-09-12 00:00:00
abstract::Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bioimaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on the Pepino mosaic virus (PepMV), a relative of the establishe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01365
更新日期:2019-01-14 00:00:00
abstract::The molecular recognition features of a DNA-sensitive fluorescent bioconjugate capable of targeting a specific DNA sequence with high efficiency are described. The bioconjugate combines a polypeptide from the Tc3 transposase DNA-binding domain with the dsDNA-sensitive fluorophore thiazole orange. Fluorescence spectros...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070334h
更新日期:2007-11-01 00:00:00
abstract::Chitin nanocrystal aqueous dispersions were prepared by acid hydrolysis of crude chitin from crab shells. The resulting dispersions were studied with small deformation oscillatory experiments and polarized optical microscopy under different conditions of nanocrystal concentration, ionic strength, pH, and temperature. ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm901046c
更新日期:2010-01-11 00:00:00
abstract::Receptor-mediated, cell-specific delivery of siRNA enables silencing of target genes in specific tissues, opening the door to powerful therapeutic options for a multitude of diseases. However, the development of delivery systems capable of targeted and effective siRNA delivery typically requires multiple steps and the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm200485b
更新日期:2011-07-11 00:00:00
abstract::We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO(2)), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900589r
更新日期:2009-10-12 00:00:00
abstract::The proteinaceous material present in beta-lactoglobulin fibrils formed after heating (20 h at 85 degrees C) at pH 2 was identified during this study. Fibrils were separated from the nonaggregated material, and the fibrils were dissociated using 8 M guanidine chloride and 0.1 M 1,4-dithiothreitol (pH 8). Characterizat...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7014224
更新日期:2008-05-01 00:00:00
abstract::The need for thermosets from renewable resources is continuously increasing to find eco-friendly alternatives to petroleum-derived materials. Products obtained from biomass have shown to play an important role in this challenge. Here, we present the structural characterization of new biobased thermosets made of humins...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01248
更新日期:2020-02-10 00:00:00
abstract::Electrospinning is a versatile process used to prepare micro- and nano- sized fibers from various polymers dissolved in volatile solvents. In this report, cellulose and cellulose-heparin composite fibers are prepared from nonvolatile room temperature ionic liquid (RTIL) solvents by electrospinning. RTILs are extracted...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050837s
更新日期:2006-02-01 00:00:00
abstract::A major challenge in drug delivery is the internalization through the apical plasma membrane of the polarized epithelial cells lining organs facing the external environment, e.g., lungs and the gastrointestinal tract. The reduced permeation of drugs entering through this pathway is in part due to the mucosal barrier a...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm700535p
更新日期:2008-02-01 00:00:00
abstract::In this study, we demonstrate for the first time the fabrication of carboxylated chitosan nanocrystals (ChsNC) with high degree of deacetylation (DDA) at >80% and narrow size distribution. We also studied its application as a sustainable support material for metal-based catalysts. Carboxylated chitin nanocrystals (ChN...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00201
更新日期:2020-06-08 00:00:00
abstract::We created the dual-responsive nanovehicle that can effectively combine and abundantly utilize magnetic and glutathione (GSH)-reductive triggers to control the drug delivery and achieve more intelligent and powerful targeting. In the nanovehicles, paramagnetic fullerene (C60@CTAF) was prepared via one-step modificatio...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01939
更新日期:2017-03-13 00:00:00
abstract::Polymerized high internal phase emulsions (polyHIPEs) have been utilized in the creation of injectable scaffolds that cure in situ to fill irregular bone defects and potentially improve tissue healing. Previously, thermally initiated scaffolds required hours to cure, which diminished the potential for clinical transla...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500754r
更新日期:2014-08-11 00:00:00
abstract::This study presents an efficient and systematic approach to synthesize bioapplicable porous hollow polymeric capsules (HPCs). The hydroxyl-functionalized nanoporous polymers with hollow capsular shapes could be generated via the moderate Friedel-Crafts reaction without using any hard or soft template. The numerous pri...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c01520
更新日期:2020-12-17 00:00:00
abstract::This paper introduces a chitosan microneedle patch for efficient and sustained transdermal delivery of hydrophilic macromolecules. Chitosan microneedles have sufficient mechanical strength to be inserted in vitro into porcine skin at approximately 250 μm in depth and in vivo into rat skin at approximately 200 μm in de...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301293d
更新日期:2012-12-10 00:00:00
abstract::Self-assembling amphiphilic designer peptides have been successfully applied as nanomaterials in biomedical applications. Understanding molecular interactions at the peptide-membrane interface is crucial, since interactions at this site often determine (in)compatibility. The present study aims to elucidate how model m...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.6b01089
更新日期:2016-11-14 00:00:00
abstract::First generation, photocrosslinkable dendrimers consisting of natural metabolites (i.e., succinic acid, glycerol, and beta-alanine) and nonimmunogenic poly(ethylene glycol) (PEG) were synthesized divergently in high yields using ester and carbamate forming reactions. Aqueous solutions of these dendrimers were photocro...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm800658x
更新日期:2008-10-01 00:00:00