Abstract:
:The single and triple helical organization of protein chains strongly influences the mechanical properties of gelatin-based materials. A chemical method for obtaining different degrees of helical organization in gelatin is covalent functionalization, while a physical method for achieving the same goal is the variation of the drying conditions of gelatin solutions. Here we explored how the introduction of desaminotyrosine (DAT) and desaminotyrosyl tyrosine (DATT) linked to lysine residues of gelatin influenced the kinetics and thermodynamic equilibrium of the helicalization process of single and triple helices following different drying conditions. Drying at a temperature above the helix-to-coil transition temperature of gelatin (T > T(c), called v(short)) generally resulted in gelatins with relatively lower triple helical content (X(c,t) = 1-2%) than lower temperature drying (T < T(c), called v(long)) (X(c,t) = 8-10%), where the DAT(T) functional groups generally disrupted helix formation. While different helical contents affected the thermal transition temperatures only slightly, the mechanical properties were strongly affected for swollen hydrogels (E = 4-13 kPa for samples treated by v(long) and E = 120-700 kPa for samples treated by v(short)). This study shows that side group functionalization and different drying conditions are viable options to control the helicalization and macroscopic properties of gelatin-based materials.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Zaupa A,Neffe AT,Pierce BF,Nöchel U,Lendlein Adoi
10.1021/bm101029ksubject
Has Abstractpub_date
2011-01-10 00:00:00pages
75-81issue
1eissn
1525-7797issn
1526-4602journal_volume
12pub_type
杂志文章abstract::We report that the continuous assembly of polymers (CAP) approach, mediated by ring-opening metathesis polymerization (ROMP), is a facile and versatile technology to prepare engineered nanocoatings for various biomedical applications. Low-fouling coatings on particles were obtained by the formation of multicomposition...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm400680d
更新日期:2013-08-12 00:00:00
abstract::Understanding the self-assembly mechanism of caged proteins provides clues to develop their potential applications in nanotechnology, such as a nanoscale drug delivery system. The E2 protein from Bacillus stearothermophilus , with a virus-like caged structure, has drawn much attention for its potential application as...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201587q
更新日期:2012-03-12 00:00:00
abstract::We investigate the effects of mixing a colloidal suspension of tunicate-derived cellulose nanocrystals (t-CNCs) with aqueous colloidal suspensions of two protein diblock copolymers, EC and CE, which bear two different self-assembling domains (SADs) derived from elastin (E) and the coiled-coil region of cartilage oligo...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401304w
更新日期:2013-12-09 00:00:00
abstract::The polymerization of N-isopropylacrylamide (NIPAAm) with ionizable monomers results in pH-responsive lower critical solution temperature (LCST) polymer which works in an ionization-dependent manner. However, gradual ionization of the comonomer occurs at a broad pH range due to the electrostatic field generated by the...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01465
更新日期:2019-04-08 00:00:00
abstract::Thin films of a biocompatible and nonbiofouling poly(oligo(ethylene glycol) methacrylate) ( pOEGMA) with various thicknesses were formed on gold and Si/SiO 2 substrates by a combination of the formation of self-assembled monolayers (SAMs) terminating in bromoester-an initiator of atom transfer radical polymerization (...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7009043
更新日期:2007-12-01 00:00:00
abstract::We have developed herein an engineered polymer-based nanoplatform showing the convergence of two-photon fluorescence imaging and bimodal phototherapeutic activity in a single nanostructure. It was achieved through the appropriate choice of three different components: a β-cyclodextrin-based polymer acting as a suitable...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm500156z
更新日期:2014-05-12 00:00:00
abstract::A new, convenient pathway is developed to synthesize highly hydrolytically labile poly(ortho ester amide) (POEA) copolymers that overcomes some of the major weaknesses of the traditional methods of synthesizing poly(ortho esters) and their derivatives. A diamine monomer containing a built-in, stabilized ortho ester gr...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm9000475
更新日期:2009-04-13 00:00:00
abstract::The electrospinning of polymer melts can offer an advantage over solution electrospinning, in the development of layered tissue constructs for tissue engineering. Melt electrospinning does not require a solvent, of which many are cytotoxic in nature, and the use of nonwater soluble polymers allows the collection of fi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050777q
更新日期:2006-03-01 00:00:00
abstract::Thymic epithelial cells (TECs) are the main regulators of T lymphocyte development and selection, requiring a three-dimensional (3D) environment to properly perform these biological functions. The aim of this work was to develop a 3D culture substrate that allows the survival and proliferation of TECs. Thus, electrosp...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00933
更新日期:2020-12-14 00:00:00
abstract::The purpose of this study was to gain mechanistic insights into the effect of different formulation parameters on the degradation and release behavior of protein-loaded nanoparticulate carrier systems based on an aliphatic polyester with pendant hydroxyl groups, poly(lactic-co-glycolic-hydroxymethyl glycolic acid) (pL...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm301900t
更新日期:2013-04-08 00:00:00
abstract::A revised crystal structure for mercerized cellulose based on high-resolution synchrotron X-ray data collected from ramie fibers is reported (space group P2(1), a = 8.10(3) A, b = 9.03(3) A, c = 10.31(5) A, gamma = 117.10(5) degrees; 751 reflections in 304 composite spots; theta < 21.11 degrees; lambda = 0.7208 A; LAL...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm005612q
更新日期:2001-07-01 00:00:00
abstract::An artificial spinning system using regenerated silk fibroin solutions is adopted to produce high-performance silk fibers. In previous studies, alcohol-based agents, such as methanol or ethanol, were used to coagulate silk dope solutions, producing silk fiber with poor mechanical properties compared with those of nati...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b00232
更新日期:2018-06-11 00:00:00
abstract::Polyamines are essential molecules supporting the structure, conformation, and function of nucleic acids and proteins. We studied stereoisomers of alpha,alpha'-dimethylated spermine [(R,R)-Me(2)Spm, (S,S)-Me(2)Spm, (R,S)-Me(2)Spm] for their ability to provoke DNA condensation and protect DNA from damage. (R,R)- and (R...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm900958c
更新日期:2010-01-11 00:00:00
abstract::The mechanical and melt flow properties of two thermoplastic potato starch materials with different amylose contents were evaluated. The materials were prepared by mixing starch, glycerol, and water, mainly in the weight proportions of 10:3:4.5. Compression molding was used to produce sheets/films with a thickness in ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050804c
更新日期:2006-03-01 00:00:00
abstract::Light-harvesting antenna core (LH1-RC) complexes isolated from Rhodoseudomonas palustris were self-assembled on a gold electrode modified with self-assembled monolayers (SAMs) of the alkanethiols NH2(CH2)nSH, n = 2, 6, 8, 11; HOOC(CH2)7SH; and CH3(CH2)7SH, respectively. Adsorption of the LH1-RC complexes on the SAMs d...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm070352z
更新日期:2007-08-01 00:00:00
abstract::Spiders spin their silk from an aqueous solution to a solid fiber in ambient conditions. However, to date, the assembly mechanism in the spider silk gland has not been satisfactorily explained. In this paper, we use molecular dynamics simulations to model Nephila clavipes MaSp1 dragline silk formation under shear flow...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01246
更新日期:2016-02-08 00:00:00
abstract::Thermoresponsive polymers with lower critical solution temperatures (LCSTs) are of significant interest for a wide range of applications from sensors to drug delivery vehicles. However, the most widely investigated LCST polymers have nondegradable backbones, limiting their applications in vivo or in the environment. D...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.0c00899
更新日期:2020-09-14 00:00:00
abstract::A novel pH and temperature sensitive block copolymer was prepared by adding pH sensitive moiety to temperature sensitive block copolymer. This block copolymer solution showed a reversible sol-gel transition by a small pH change in the range of pH 7.4-8.0 and also by the temperature change in the region of body tempera...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050521k
更新日期:2005-11-01 00:00:00
abstract::Dextran surface grafting density was systematically varied via a two-step process involving SiO(2) amination by aminopropyltriethoxy silane (APTES) followed by oxidized dextran (M(w) = 110 kDa) chemisorption. Dextran oxidation kinetics with sodium metaperiodate (NaIO(4)) were quantified by (1)H NMR and pH measurements...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm050601o
更新日期:2006-02-01 00:00:00
abstract::Placing a physical barrier between the injured site and the adjacent tissues is a very common and highly effective approach to prevent abdominal adhesions in these days. A biodegradable trilayered barrier was fabricated to prevent formation of abdominal adhesions, in which a poly(lactide-co-glycolide)/poly(lactide)-b-...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.5b01099
更新日期:2015-09-14 00:00:00
abstract::Nanoparticles with high aspect ratios have favorable attributes for drug delivery and bioimaging applications based on their enhanced tissue penetration and tumor homing properties. Here, we investigated a novel filamentous viral nanoparticle (VNP) based on the Pepino mosaic virus (PepMV), a relative of the establishe...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.8b01365
更新日期:2019-01-14 00:00:00
abstract::The basic physicochemical properties that determine the distribution and fate of synthetic macromolecules in living cells were characterized using fluorescently labeled HPMA (N-(2-hydroxypropyl)methacrylamide) copolymers. Twelve different classes of water-soluble copolymers were created by incorporating eight differen...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm801514x
更新日期:2009-07-13 00:00:00
abstract::The leading cause of death in the United States is cardiovascular disease. The majority of these cases result from heart failure post-myocardial infarction (MI). We present data providing evidence for use of acetalated dextran (AcDex) microparticles as a delivery vehicle for therapeutics to the heart post-MI. We harne...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm401050j
更新日期:2013-11-11 00:00:00
abstract::Cationic kraft lignin (CKL) macromolecules were produced via polymerizing kraft lignin (KL) with [2-(acryloyloxy)ethyl]trimethylammonium chloride (ATAC) or [2-(methacryloyloxy)ethyl]trimethylammonium methyl sulfate (METAM). Despite slightly different charge densities (2.3-2.5 mmol/g) of CKL, lignin-METAM (KL-METAM) ha...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/acs.biomac.9b01016
更新日期:2019-10-14 00:00:00
abstract::Enzymatic hydrolysis of selected copolymers of cellulose diacetate-graft-poly(L-lactide)s (CDA-g-PLLAs) were conducted with proteinase K for film specimens, which were solely quenched from the molten state or, further, annealed at temperatures below or above their glass transition temperatures. The hydrolysis rates de...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm034453i
更新日期:2004-03-01 00:00:00
abstract::The nonapeptide hemopressin, which is derived from the α chain of hemoglobin, has been reported to exhibit inverse agonist activity against the CB1 receptor. Administration of this peptide in animal models led to decreased food intake and elicited hypotensive and antinociceptive effects. On the basis of hemopressin's ...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm201836f
更新日期:2012-03-12 00:00:00
abstract::Eosinophil cationic protein (ECP) is an antimicrobial protein belonging to the superfamily of RNase A. ECP exhibits a broad spectrum of action against bacteria and, at higher concentrations, displays cytotoxic activity to eukaryotic cells. Recently, a powerful aggregation activity for lipid vesicles and for the gram-n...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm100334u
更新日期:2010-08-09 00:00:00
abstract::A porous scaffold as a cell-compatible material was designed and prepared using a phospholipid copolymer composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate, and enantiomeric macromonomers, the poly(L-lactic acid) (PLLA) macromonomer, and poly(D-lactic acid) (PDLA) macromonomer. On the bas...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm025586r
更新日期:2002-09-01 00:00:00
abstract::Polystyrene-polyisocyanopeptide (PS-PIAT) polymersomes containing CALB in two different locations, one in the aqueous inner compartment and one in the bilayer, were investigated for enzymatic ring-opening polymerization of lactones in water. It is shown that the monomers 8-octanolactone and dodecalactone yield oligome...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm7005938
更新日期:2007-12-01 00:00:00
abstract::Forisomes are protein polymers found in leguminous plants that have the remarkable ability to undergo reversible "muscle-like" contractions in the presence of divalent cations and in extreme pH environments. To gain insight into the molecular basis of forisome structure and assembly, we used confocal laser scanning mi...
journal_title:Biomacromolecules
pub_type: 杂志文章
doi:10.1021/bm3008499
更新日期:2012-10-08 00:00:00