Abstract:
:Midkine (MK) is a heparin binding growth factor identified as a product of a retinoic acid-responsive gene; it is frequently expressed at high levels in many human carcinomas. Although the expression of the mRNA encoding truncated MK (tMK) in unique human cancer cells has been reported, the tMK polypeptide itself has not yet been identified. In order to clarify the biological role of tMK, recombinant tMK was expressed in Escherichia coli and purified. Recombinant tMK was purified as a single band in SDS-PAGE under reducing conditions showing an apparent molecular mass of 10 kDa. Purified recombinant tMK showed the same extent of proliferative activity towards Wilms' tumor (G401) cells as full length human MK. These results suggest that the structure of this recombinant tMK is same as the native polypeptide.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Paul S,Mitsumoto T,Yamamoto I,Shinozawa Tdoi
10.1016/s0304-3835(00)00695-9keywords:
subject
Has Abstractpub_date
2001-02-26 00:00:00pages
239-44issue
2eissn
0304-3835issn
1872-7980pii
S0304383500006959journal_volume
163pub_type
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