A rapid, sensitive assay for cocaine and its metabolites in biological fluids using solid-phase extraction and high-performance liquid chromatography.

abstract：:A rapid and simple method is described for the separation of quinidine (Q), its metabolites 3-hydroxyquinidine (30HQ) and 2'oxoquinidinone (QD) as well as the quinidine salt contaminant, dihydroquinidine (DHQ) in plasma by high pressure liquid chromatography (HPLC). A silica column was used with methanol: 1 N ammonium...

journal_title：Journal of analytical toxicology

authors： Pershing LK,Peat MA,Finkle BS

更新日期：1982-05-01 00:00:00

abstract：:In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of...

journal_title：Journal of analytical toxicology

authors： Blomme B,Lheureux P,Gerlo E,Maes V

更新日期：2001-03-01 00:00:00

abstract：:Exemplars of current commercial breath-alcohol simulators were studied to ascertain their suitability for control tests and as calibrators in breath-alcohol analysis. Temperature, effluent volumes and pressures, effluent-alcohol concentration, and alcohol depletion were measured for simulators coupled with various cur...

journal_title：Journal of analytical toxicology

authors： Dubowski KM,Essary NA

更新日期：1991-09-01 00:00:00

abstract：:A protocol is described for the preparation and characterization of bovine blood lead (Pb) reference material (RM). Adult bovine animals are orally dosed with Pb as Pb(NO3)2, and blood is collected directly from the jugular vein into 5-mL evacuated, low-Pb glass tubes containing EDTA as anticoagulant. The blood is coo...

journal_title：Journal of analytical toxicology

authors： Cox DH,Duncan CE,Wyatt GP,Meadow JS,Robinson JB

更新日期：1989-07-01 00:00:00

abstract：:An automated column-switching liquid chromatographic-atmospheric pressure chemical ionization-mass spectrometric (LC-APCI-MS) method for the simultaneous determination of frequently encountered benzodiazepine hypnotics and their relevant metabolites in urine has been established. A column-switching online solid-phase ...

journal_title：Journal of analytical toxicology

authors： Miki A,Tatsuno M,Katagi M,Nishikawa M,Tsuchihashi H

更新日期：2002-03-01 00:00:00

abstract：:A method for the detection and quantitation of methamphetamine and its major metabolite in hair, nails, sweat, and saliva from habitual users of methamphetamine by mass fragmentography has been developed. Hair and nail samples were washed with water and methanol to remove the external contamination, processed with 0.6...

journal_title：Journal of analytical toxicology

authors： Suzuki S,Inoue T,Hori H,Inayama S

更新日期：1989-05-01 00:00:00

abstract：:A high performance liquid chromatographic method is reported, which incorporates three internal standards (I-cinchonidine, N-propylprocainamide, and para-chlorodisopyramide) for the simultaneous quantitation of four commonly prescribed antiarrhythmic drugs: quinidine, procainamide, N-acetylprocainamide, and disopyrami...

journal_title：Journal of analytical toxicology

authors： Bridges RR,Jennison TA

更新日期：1983-03-01 00:00:00

abstract：:A sensitive and specific method for the quantitative determination of D,L-methadone (MD) and its metabolites, D,L-2-ethyl-1,5-dimethyl-3, 3-diphenylpyrrolinium (EDDP) and D,L-2-ethyl-5-methyl-3, 3-diphenyl-1-pyrroline (EMDP), in hair has been developed. Deuterated internal standards of MD, EMDP, and EDDP were added to...

journal_title：Journal of analytical toxicology

authors： Wilkins DG,Nagasawa PR,Gygi SP,Foltz RL,Rollins DE

更新日期：1996-10-01 00:00:00

abstract：:This report describes a gas chromatographic procedure using a packed column and a flame ionization detector, which is suitable for the therapeutic monitoring of N-methylformamide (N-MF). N-MF is a polar compound that induces cancer cell maturation in vitro and exhibits antitumor activity with human tumors xenografted ...

journal_title：Journal of analytical toxicology

authors： Griffiths WC,Matook GM,Dexter DL,Crabtree GW,Spremulli EN,Calabresi P,Diamond I

更新日期：1983-03-01 00:00:00

abstract：:Previous studies have shown that drug concentrations in blood can change during storage, especially at room temperature, but even labile drugs such as cocaine may be stable in dried blood spots (DBS). A new method has been developed for the analysis of hydrolytically labile drugs in blood spots on filter paper in orde...

journal_title：Journal of analytical toxicology

authors： Alfazil AA,Anderson RA

更新日期：2008-09-01 00:00:00

abstract：:This study describes a matrix-assisted laser-desorption-ionization (MALDI) mass spectrometry for rapid screening of 12 diuretics in spiked urine. C60 is used as the matrix for MALDI. Diuretics are directly analyzed by C60-MALDI without previous derivatization. The fact that most diuretic molecules contain sulfate grou...

journal_title：Journal of analytical toxicology

authors： Huang JP,Yuan CH,Shiea J,Chen YC

更新日期：1999-09-01 00:00:00

abstract：:The Abbott TDx Urine Amphetamine/Methamphetamine II fluorescence polarization immunoassay technique was applied to the determination of D-methamphetamine in hemolyzed whole blood. The assay was found to have 100% cross-reactivity with D-methamphetamine and only an 8% cross-reactivity with L-methamphetamine. Whole bloo...

journal_title：Journal of analytical toxicology

authors： Simonick TF,Watts VW

更新日期：1992-03-01 00:00:00

abstract：:A rapid and sensitive solid-phase extraction method for the lysergic acid diethylamide (LSD) metabolite 2-oxo-3-hydroxy-LSD (O-H-LSD) was developed for use in a forensic laboratory. The method uses a positive-pressure manifold anion-exchange polymer-based solid-phase extraction with subsequent analysis by liquid chrom...

journal_title：Journal of analytical toxicology

authors： Horn CK,Klette KL,Stout PR

更新日期：2003-10-01 00:00:00

abstract：:Amoxapine, a tricyclic antidepressant, is metabolized to 8-hydroxyamoxapine and 7-hydroxyamoxapine. There are few reports on the metabolism of this drug and correlation of clinical symptoms in overdose patients. Five such patients admitted to the Emergency Unit of the University of Cincinnati Hospital were studied. Cl...

journal_title：Journal of analytical toxicology

authors： Tasset JJ,Pesce AJ

更新日期：1984-05-01 00:00:00

abstract：:The aim of this study was to investigate the early-phase postmortem redistribution of the enantiomers of citalopram (CIT) and its metabolites demethylcitalopram (DCIT) and didemethylcitalopram (DDCIT) in a rat model. Furthermore, we wanted to examine the role of the lungs as a reservoir of postmortem drug release and ...

journal_title：Journal of analytical toxicology

authors： Kugelberg FC,Kingbäck M,Carlsson B,Druid H

更新日期：2005-05-01 00:00:00

abstract：:A variety of chemical agents were evaluated to determine their effects on fluorescence polarization immunoassays for drugs of abuse. Sixteen different agents, at concentrations up to 10%, were tested against urine assays for cannabinoids, cocaine (metabolite), amphetamines, opiates, phencyclidine (PCP), and barbiturat...

journal_title：Journal of analytical toxicology

authors： Schwarzhoff R,Cody JT

更新日期：1993-01-01 00:00:00

abstract：:A novel mass spectrometry detection technique based on a multi-period and multi- experiment (MRM-EPI-MRM3) with library matching in a single run for fast and rapid screening and identification of amphetamine type stimulants (ATS) related drugs in whole blood, urine and dried blood stain was developed and validated. Th...

journal_title：Journal of analytical toxicology

authors： Zubaidi FA,Choo YM,Tan GH,Hamid HA,Choy YK

更新日期：2019-08-23 00:00:00

abstract：:Storage of drug-based evidence inside sealed safes may allow chemical vapors to accumulate, creating concerns of drug exposure by inhalation, or the possibility of cross-contamination of drug evidence. Air samples were taken from inside eight drug safes and one small storage room at nine city and country police statio...

journal_title：Journal of analytical toxicology

authors： Doran GS,Deans R,De Filippis C,Kostakis C,Howitt JA

更新日期：2018-06-01 00:00:00

abstract：:We described a simple procedure for the flash heater methylation of valproic acid and cyclohexylacetic acid (internal standard) by gas-liquid chromatography (GLC). A small volume (100 microL) of sample is acidified with 10% perchloric acid and extracted into hexane. An aliquot is back extracted into 5% tetramethylammo...

journal_title：Journal of analytical toxicology

authors： Calendrillo BA,Reynoso G

更新日期：1980-11-01 00:00:00

abstract：:A simple, rapid ethanol screen based on a microdiffusion technique (Analytical Systems, Marion Laboratories) has been evaluated and compared to an established enzymatic procedure (Abbott TDx). They showed agreement in 484 of 488 biological specimens and all control specimens. They disagreed on 4 specimens--3 positive ...

journal_title：Journal of analytical toxicology

authors： Serrano L,Capshaw B,Quattrocchi F

更新日期：1988-11-01 00:00:00

abstract：:Bleach, nitrite, chromate, and hydrogen peroxide-peroxidase are effective urine adulterants used by the illicit drug users to conceal marijuana-positive results. Methods for detecting nitrite and chromate are available. Effects of other oxidizing agents that could possibly be used as adulterants and are difficult to d...

journal_title：Journal of analytical toxicology

authors： Paul BD,Jacobs A

更新日期：2002-10-01 00:00:00

abstract：:Improvements have been made in recent years in on-site or point-of-care (POC) immunoassay testing devices. We evaluated the DrugCheck 9 cup, a new qualitative visually read, competitive binding, immunoassay cup that measures nine analytes. The study was performed according to the recent National Laboratory Certificati...

journal_title：Journal of analytical toxicology

authors： Taylor EH,Pizzo P

更新日期：2004-04-01 00:00:00

abstract：:Positive benzoylecgonine (BZE) urinalysis results are sometimes challenged in legal and administrative proceedings on the grounds that the presence of BZE is due to the addition of cocaine to the urine sample with subsequent in vitro hydrolysis to BZE. Consequently, counsel for the respondent or defendant may move tha...

journal_title：Journal of analytical toxicology

authors： Klette KL,Poch GK,Czarny R,Lau CO

更新日期：2000-10-01 00:00:00

abstract：:The analysis of biological specimens collected at autopsy for the presence of exogenous insulin(s) is of special interest in select death investigations as they may be suspected in the cause of a death. Technical challenges include the limited stability of insulin, and the forensic requirement of differentiating endog...

journal_title：Journal of analytical toxicology

authors： Legg KM,Labay LM,Aiken SS,Logan BK

更新日期：2019-08-23 00:00:00

abstract：:A colchicine blood concentration 250 microgram/L was determined in a 39-year-old white female 2 hours following the ingestion of an oral overdose of the drug. At death, 40 hours following ingestion, no drug was detected. Colchicine was extracted from buffered blood using dichloromethane. The evaporated extract was red...

journal_title：Journal of analytical toxicology

authors： Caplan YH,Orloff KG,Thompson BC

更新日期：1980-05-01 00:00:00

abstract：:This study shows a simple, rapid, and specific method for the quantitative determination of cyanide ion in body fluids, especially blood, by fluorimetry. It is based upon the transformation of cyanide ion into hydrocyanic acid, which then reacts with 2,3-naphthalenedialdehyde and taurine in a self-contained system. Th...

journal_title：Journal of analytical toxicology

authors： Felscher D,Wulfmeyer M

更新日期：1998-09-01 00:00:00

abstract：:Radioactive methadone, phenytoin, phenobarbital, and diazepam were easily and efficiently removed from human urine samples by adsorption on C18 bonded silica phases. These techniques were simpler than conventional extractions at pH 9.3 or 5.2 with dichloromethane, XAD-2 and methanol, or Celite 560 and dichloromethane....

journal_title：Journal of analytical toxicology

authors： Hyde PM

更新日期：1985-11-01 00:00:00

abstract：:A blood concentration of tetrahydrocannabinol (THC) in the low nanograms-per-milliliter range is often claimed to result from drug use more than 24-48 h previously. The present investigation determined concentrations of cannabinoids in blood collected at least 24 h from smoking in an in-patient setting. During samplin...

journal_title：Journal of analytical toxicology

authors： Skopp G,Pötsch L

更新日期：2008-03-01 00:00:00

abstract：:The synthetic chemical 4-aminobiphenyl (4-ABP) has been shown to be a bladder carcinogen in both man and animals. A valid radioimmunoassay for a metabolite of 4-ABP has been developed as a means to monitor potential human exposure to 4-ABP. The antibody was produced by the immunization of two female New Zealand White ...

journal_title：Journal of analytical toxicology

authors： Johnson HJ Jr,Cernosek SF Jr,Gutierrez-Cernosek RM,Brown LL

更新日期：1980-03-01 00:00:00

abstract：:An assay for neurotoxic esterase (neuropathy target esterase, NTE) was developed by Johnson (1,2) to assess the delayed neurotoxic potential of organophosphorus compounds. NTE activity is calculated from the rate of phenyl valerate hydrolysis resistant to paraoxon and sensitive to mipafox inhibition under specified co...

journal_title：Journal of analytical toxicology

authors： Kayyali US,Moore TB,Randall JC,Richardson RJ

更新日期：1991-03-01 00:00:00