Abstract：
:We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Furthermore, in general, expression of the transgene showed developmental regulation similar to that of the endogenous gene. However, the precise location of cellular expression could not be determined in this model. To overcome this limitation, we have developed a similar model, but replaced CAT with the reporter enzyme beta-galactosidase. Enzyme activity was readily detected in the transgenic mouse embryos in expected regions of tissue forming elastic fibers, including the dermis and elastic cartilage. Of considerable interest, however, was the novel finding of expression in specific areas of neuroepithelium of the brain and in the perichondrium surrounding areas destined to form hyaline cartilage in endochondral bone formation. These latter areas included all the bones of the limbs, the spine and rib cage. It appeared that these segments of elastin expression demarcated the border between the developing cartilage and the surrounding mesenchymal tissue. Elastin promoter expression was also found in developing somites, in the mesenchymal layer of the forming cornea of the eye, in the genital tubercle and in the epithelium destined to form the olfactory epithelium. These findings indicate that the elastin promoter is activated during embryonic development in a variety of tissues, suggesting that elastin gene expression may play a role in organizing cutaneous, skeletal and neural structures.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Lakkakorpi J,Li K,Decker S,Korkeela E,Piddington R,Abrams W,Bashir M,Uitto J,Rosenbloom Jdoi
10.3109/03008209909029111keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
155-62issue
2eissn
0300-8207issn
1607-8438journal_volume
40pub_type
杂志文章abstract：:Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2020.1759562
更新日期：2020-05-05 00:00:00
abstract：:We have investigated the changes in some of the biochemical and biophysical properties of human femoral head cartilage on the one hand during ageing and on the other hand in osteoarthritis. Topographical variations were also investigated. The parameters studied were those relevant to cartilage function, viz., proteogl...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208909043895
更新日期：1989-01-01 00:00:00
abstract：:To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model. Specifically, immunohis...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209809021505
更新日期：1998-01-01 00:00:00
abstract：:The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2020.1751140
更新日期：2020-04-20 00:00:00
abstract：:Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium and mesenchyme, while Shh transcripts are present in dental epithelium only, suggesting that SHH functions as an a...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Effects of applied hydrostatic pressure on transmembrane potentials were investigated in sheep articular chondrocytes and human skin fibroblasts in non-confluent monolayer cultures. Resting potentials in chondrocytes (about -12 mv) and in fibroblasts (about -15 mV) were increased and decreased respectively by over 40%...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209209014227
更新日期：1992-01-01 00:00:00
abstract：:The in-vitro fluoride treatment technique has been introduced to investigate the composite behavior of bone tissue. Bone tissue with different mechanical properties can be obtained by varying the concentration, pH and immersion time in fluoride ion solutions. The chemical and physical changes in intact pieces of bone ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The paper reviews the changes in ameloblast ultrastructure, concomitant with the changes in its functions across the major stages of amelogenesis. It describes the mechanisms associated with the major events in biosynthesis and degradation of the major enamel proteins (amelogenins and tuftelin/enamelins) and with the ...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：10.3109/03008209509013710
更新日期：1995-01-01 00:00:00
abstract：:Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2019.1702031
更新日期：2020-05-01 00:00:00
abstract：:We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208309015011
更新日期：1983-01-01 00:00:00
abstract：:We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209109006995
更新日期：1991-01-01 00:00:00
abstract：:Short term cultures were carried out with chondrocytes and tissue fragments from fetal guinea pig epiphyseal cartilage. Proteoglycans were isolated from these cultures and their properties were compared with those of proteoglycans from adult hyaline cartilage. It was concluded that the proteoglycans synthesized in cul...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207809152274
更新日期：1978-01-01 00:00:00
abstract：:Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular pro...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：10.3109/03008209009152421
更新日期：1990-01-01 00:00:00
abstract：:The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209109152166
更新日期：1991-01-01 00:00:00
abstract：:A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209509013726
更新日期：1995-01-01 00:00:00
abstract：:Purpose/Aim: Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from vascular smooth muscle cells significantly contribute to the initiation and progression of this pathology. Recently, we have demonstrated that elevated p...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1444759
更新日期：2018-12-01 00:00:00
abstract：:Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200590935510
更新日期：2005-01-01 00:00:00
abstract：:A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209109029158
更新日期：1991-01-01 00:00:00
abstract：:Odontogenesis involves a complex series of processes including epithelial-mesenchymal interactions, morphogenesis, differentiation, fibrillogenesis, and mineralization. Extracellular (ECM) remodeling plays a critical role in the rapid morphological changes that accompany these events. It is proposed that matrix metall...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200290000538
更新日期：2002-01-01 00:00:00
abstract：:The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208309004860
更新日期：1983-01-01 00:00:00
abstract：:Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2013.775258
更新日期：2013-01-01 00:00:00
abstract：:Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorl...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2014.996702
更新日期：2015-01-01 00:00:00
abstract：:Currently, the mechanism governing the regeneration of the soft tissue-to-bone interface, such as the transition between the anterior cruciate ligament (ACL) and bone, is not known. Focusing on the ACL-to-bone insertion, this study tests the novel hypothesis that interactions between cells from the ligament (fibroblas...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2016.1230106
更新日期：2016-11-01 00:00:00
abstract：:EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200290000880
更新日期：2002-01-01 00:00:00
abstract：:The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207809152627
更新日期：1978-01-01 00:00:00
abstract：:The meniscal cartilages in the knee function to improve congruity of the medial and lateral femoro-tibial joints and play critical roles in load distribution and joint stability. Meniscal tears of various configurations are one of the most common conditions of the knee and are associated with an increased risk of deve...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：10.1080/03008207.2017.1284824
更新日期：2017-01-01 00:00:00
abstract：:The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008200009067666
更新日期：2000-01-01 00:00:00
abstract：:Growth and differentiation factor-5 (GDF-5) is known to induce tendon tissue and stimulate tendon healing. The hypothesis was that adenoviral GDF-5 transfer leads to transitory transgene expression and improves Achilles tendon healing. In vitro experiments were first performed with rat tenocytes. Transgene expression ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200500237120
更新日期：2005-01-01 00:00:00
abstract：:In our previous developmental study on skin we reported that correlations existed between the collagen fibril diameter distribution, glycosaminoglycan content and composition, and mechanical role. In this work we present new but related electron microscope data on the ultrastructure of skins from flounder, marlin, cod...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208709006977
更新日期：1987-01-01 00:00:00
