Abstract:
:We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appropriate methods. Type I procollagen was the major form of collagen secreted into the medium by normal cells cultured from one mid-term fetus, infants (n = 3), children (n = 3), adolescents (n = 2), and adults (n = 3). Interstrain differences in collagen production under standardized conditions were significantly greater than intrastrain variation (anova, p = 0.0051). There was no significant alteration in the type I:type III collagen ratio due to variation in: phase of cell growth, doublings (between 13th and 22nd), rate of isotope incorporation, labelling time (24-72 hrs) in the presence of ascorbic acid (50 micrograms/ml), age of donor (with the possible exception of adolescence), and site of biopsy (genital and non-genital sites). Variable conversion of type I procollagen to collagen did not perturb the type I:type III collagen ratio. Cell strains from OI patients (Sillence classification): type I (one strain); type II, III and IV (3 strains each) had greater interstrain than intrastrain variation in the collagen ratio (p = 0.0149). Interstrain differences were greater in OI cell strains relative to normal cell strains (p less than 0.01). In the aggregate, OI cells had significantly lower type I collagen production relative to type III (I/III ratio = 1.18) when compared with normal cells (I/III ratio = 2.90; t test, p less than 0.0001). These findings imply abnormal synthesis, secretion or stability of type I procollagen and greater phenotypic heterogeneity in OI skin fibroblasts relative to normal cells.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Fraser J,Lancaster GA,Scriver CRdoi
10.3109/03008208309015011subject
Has Abstractpub_date
1983-01-01 00:00:00pages
57-67issue
1eissn
0300-8207issn
1607-8438journal_volume
11pub_type
杂志文章abstract::The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152627
更新日期:1978-01-01 00:00:00
abstract::The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152378
更新日期:1981-01-01 00:00:00
abstract::The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023927
更新日期:1998-01-01 00:00:00
abstract::Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.996702
更新日期:2015-01-01 00:00:00
abstract::The total and individual glycosaminoglycan (GAG) content at various stages of the Dupuytren disease process and in samples of normal palmar connective tissue (palmar dermis, palmar fascia and digital flexor tendon) from the hands of uninvolved age-matched controls have been assayed and compared. Morphological comparis...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160258
更新日期:1982-01-01 00:00:00
abstract::To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model. Specifically, immunohis...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809021505
更新日期:1998-01-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.882331
更新日期:2014-06-01 00:00:00
abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010814
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:This study proposes to evaluate, by means of microtomographic analysis, the topical potential of raloxifene combined with BioGran® through the sonochemical method in the repair of critical bone defects in the calvaria of rats. The hypothesis was that the homogenization of Raloxifene to Biogran at the 20% conc...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1430143
更新日期:2018-12-01 00:00:00
abstract::X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109160244
更新日期:1981-01-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1485666
更新日期:2018-09-01 00:00:00
abstract::Purpose: The osteocyte is considered the major mechanosensor in bone, capable of detecting forces at a cellular level to coordinate bone formation and resorption. The pathology of age-related bone loss, a hallmark of osteoporosis, is attributed in part to impaired osteocyte mechanosensing. However, real-time evidence ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1712377
更新日期:2020-05-01 00:00:00
abstract::Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1702031
更新日期:2020-05-01 00:00:00
abstract::Volume and morphology of chondrocytes in osteoarthritic human hip joint articular cartilage were characterized, and their relationship to tissue structure and function was determined. Human osteochondral articular cartilage samples (n=16) were obtained from the femoral heads of nine patients undergoing total hip arthr...
journal_title:Connective tissue research
pub_type: 临床试验,杂志文章
doi:10.3109/03008207.2014.912645
更新日期:2014-08-01 00:00:00
abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152166
更新日期:1991-01-01 00:00:00
abstract::Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase A...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709005621
更新日期:1987-01-01 00:00:00
abstract::The small leucine-rich repeat proteoglycans (SLRPs) constitute a group of structurally and functionally related molecules that participate in the organization of the extracellular matrix ECM and have important effects on cell behavior. Osteosarcomas are heterogeneous bone tumors whose common characteristic is the prod...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200802147589
更新日期:2008-01-01 00:00:00
abstract::??Although injuries to the medial collateral ligament (MCL) can heal functionally without surgical intervention, the collagen fibers in the healing tissue remain compromised. The molecular basis for this poor healing potential was investigated by examining extracellular matrix-modifying molecules such as bone morphoge...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.524719
更新日期:2011-01-01 00:00:00
abstract::Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.546536
更新日期:2011-10-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000565
更新日期:2002-01-01 00:00:00
abstract::The biological effects of isolated soluble dentin extracellular matrix components on the induction of reparative dentinogenesis in exposed cavities in ferret canine teeth have been shown to be blocked by immobilizing the extracellular matrix components on nitrocellulose or Millipore membranes during implantation. This...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013736
更新日期:1995-01-01 00:00:00
abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209309016835
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1276177
更新日期:2017-01-01 00:00:00
abstract::This study evaluated the effect of short bouts of stretching on the soleus muscle after immobilization, by measuring the birefringence of the intramuscular connective tissue (ICT) and the muscle fiber area. Thirty rats were divided into five groups: the left soleus was immobilized in the shortened position; after immo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600995940
更新日期:2006-01-01 00:00:00
abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809008059
更新日期:1988-01-01 00:00:00
abstract::Eruption requires synchrony of the tooth with the surrounding tissues, particularly the bone. One important step during eruption is remodelling of the alveolar bone at the base of the tooth and along the roots. Expression of BMP6 was reported to be increased in the basal half of the dental follicle prior to eruption a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951441
更新日期:2014-10-01 00:00:00
abstract::In the early mineralized enamel crystals, ribbon-like crystals appear near the ameloblasts. Some ribbon-like crystals showed helical or spiral structure within restricted environment during the preparation of embryonic bovine specimens for electron microscope. These specimens did not suffer from the cutting damages no...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017027
更新日期:1998-01-01 00:00:00
abstract::Glycosaminoglycan synthesis by cells cultured from nodule or band tissues of patients with Dupuytren's disease was compared with that of skin fibroblasts cultured from uninvolved areas of the palm. No differences were observed in culture which could account either for the abnormal glycosaminoglycan content or the incr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160272
更新日期:1982-01-01 00:00:00