Increased elastin mRNA levels associated with surgically induced intimal injury.


:Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative recovery, tissue samples were obtained for histopathology, electron microscopy, RNA extraction and mRNA quantitation. Using a series of mammalian cDNA clones and the procedure of slot blot hybridization, we have shown that elastin and types I and III procollagen mRNA levels rose significantly during the postoperative period following vascular distention. The increase correlated with an increase in the cell mass present in both the venous and arterial intimal layers. Changes in gamma-actin mRNA levels were also associated with this rapid proliferative response but in arterial tissue only.


Connect Tissue Res


Boyd CD,Kniep AC,Pierce RA,Deak SB,Karboski C,Miller DC,Parker MI,Mackenzie JW,Rosenbloom J,Scott GE




Has Abstract


1988-01-01 00:00:00












  • Effect of compressive force on the production of prostaglandin E(2) and its receptors in osteoblastic Saos-2 cells.

    abstract::In orthodontic tooth movement, prostaglandin E(2) (PGE(2)) released from osteoblasts can alter the normal process of bone remodeling. We examined the effect of compressive force (CF) on PGE(2) production, PGE receptors (Ep1-4) expression, phosphorylation of protein kinase A (p-PKA), and calcium content in Saos-2 cells...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Sanuki R,Mitsui N,Suzuki N,Koyama Y,Yamaguchi A,Isokawa K,Shimizu N,Maeno M

    更新日期:2007-01-01 00:00:00

  • Histochemical properties of the "crystal ghosts" of calcifying epiphyseal cartilage.

    abstract::The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Bonucci E,Silvestrini G,di Grezia R

    更新日期:1989-01-01 00:00:00

  • Electron imaging and diffraction study of individual crystals of bone, mineralized tendon and synthetic carbonate apatite.

    abstract::A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Moradian-Oldak J,Weiner S,Addadi L,Landis WJ,Traub W

    更新日期:1991-01-01 00:00:00

  • TGF beta-1 downregulates DMP-1 and DSPP in odontoblasts.

    abstract::Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Unterbrink A,O'Sullivan M,Chen S,MacDougall M

    更新日期:2002-01-01 00:00:00

  • Bone material elasticity in a murine model of osteogenesis imperfecta.

    abstract::To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Mehta SS,Antich PP,Landis WJ

    更新日期:1999-01-01 00:00:00

  • Arthrofibrosis and large joint scarring.

    abstract::Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are bei...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审


    authors: Chen AF,Lee YS,Seidl AJ,Abboud JA

    更新日期:2019-01-01 00:00:00

  • The effects of aging on the eruptive behavior of the mandibular incisor in male rats.

    abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Moxham BJ

    更新日期:2002-01-01 00:00:00

  • Evolutionary and functional significance of hominoid tooth enamel.

    abstract::The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Gantt DG,Rafter JA

    更新日期:1998-01-01 00:00:00

  • Monoclonal antibodies to heparan sulfate proteoglycan: development and application to the study of normal tissue and pathologic human kidney biopsies.

    abstract::Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Kemeny E,Fillit HM,Damle S,Mahabir R,Kefalides NA,Gregory JD,Antonovych T,Sabnis S,Zabriskie JB

    更新日期:1988-01-01 00:00:00

  • Synergistic effect of combined growth factors in porcine intervertebral disc degeneration.

    abstract::Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Cho H,Lee S,Park SH,Huang J,Hasty KA,Kim SJ

    更新日期:2013-01-01 00:00:00

  • Possible involvement of altered RGD sequence in reduced adhesive and spreading activities of advanced glycation end product-modified fibronectin to vascular smooth muscle cells.

    abstract::Although fibronectin (FN) modified by advanced glycation end products (AGEs) has been shown to contribute to the development of diabetic vascular complications through its reduced adhesive activity to vascular cells, little is known about changes in the cell binding domain of AGE-modified FN. Here we examined the mech...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Sakata N,Sasatomi Y,Meng J,Ando S,Uesugi N,Takebayashi S,Nagai R,Horiuchi S

    更新日期:2000-01-01 00:00:00

  • Detection of tubulointerstitial nephritis antigen (TIN-ag) in Lewis rat.

    abstract::Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TI...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Nelson TR,Butkowski RJ,Michael AF,Charonis AS

    更新日期:1997-01-01 00:00:00

  • Biosynthesis of sulfated proteoglycan in vitro by cells derived from human osteochondrophytic spurs of the femoral head.

    abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Malemud CJ,Moskowitz RW,Goldberg VM

    更新日期:1984-01-01 00:00:00

  • Adenovirus-mediated gene transfer of growth and differentiation factor-5 into tenocytes and the healing rat Achilles tendon.

    abstract::Growth and differentiation factor-5 (GDF-5) is known to induce tendon tissue and stimulate tendon healing. The hypothesis was that adenoviral GDF-5 transfer leads to transitory transgene expression and improves Achilles tendon healing. In vitro experiments were first performed with rat tenocytes. Transgene expression ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Rickert M,Wang H,Wieloch P,Lorenz H,Steck E,Sabo D,Richter W

    更新日期:2005-01-01 00:00:00

  • Estimation of the elastic modulus of child cortical bone specimens via microindentation.

    abstract::Purpose: Non-pathological child cortical bone (NPCCB) studies can provide clinicians with vital information and insights. However, assessing the anisotropic elastic properties of NPCCB remains a challenge for the biomechanical engineering community. For the first time, this paper provides elastic moduli values for NPC...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Semaan M,Karam E,Baron C,Pithioux M

    更新日期:2019-07-01 00:00:00

  • Studies on the mechanism of hyaluronate lyase action.

    abstract::Hyaluronate lyase from streptococci group A was purified by chromatography on DEAE-Sephadex A-50 and on Biogel P-150, thereby enriching it about 1,000-fold and separating it into two enzyme fractions with the same amino acid composition. The photooxidation of hyaluronate lyase in the presence of methylene blue results...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Greiling H,Stuhlsatz HW,Eberhard T,Eberhard A

    更新日期:1975-01-01 00:00:00

  • Interactions of elastin and microfibrils in elastogenesis of human pulmonary fibroblasts in culture.

    abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Fukuda Y,Nakazawa N,Yamanaka N

    更新日期:1993-01-01 00:00:00

  • Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.

    abstract::Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Matheson A,Regmi SC,Martin-Alarcon L,Jay GD,Scott WM,Schmidt TA

    更新日期:2020-04-20 00:00:00

  • Incomplete elongation of the chondroitin sulfate linkage region on aggrecan and response to interleukin-1β.

    abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Frankenberger C,Borgia JA,Edirisinghe PD,Oegema TR

    更新日期:2013-01-01 00:00:00

  • Stimulation of small proteoglycan synthesis by the hyaluronan synthesis inhibitor 4-methylumbelliferone in human skin fibroblasts.

    abstract::Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Funahashi M,Nakamura T,Kakizaki I,Mizunuma H,Endo M

    更新日期:2009-01-01 00:00:00

  • Induced hypothyroidism alters articular cartilage in skeletally immature miniature swine.

    abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Bundy JA,Yang JT,Morscher MA,Steiner RP,Adamczyk MJ,Weiner DS,Jacquet RD,Landis WJ

    更新日期:2020-12-11 00:00:00

  • Degradative changes in whole enamel homogenates incubated in vitro in the presence of low calcium ion concentrations.

    abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Smith CE,Chen WY

    更新日期:1998-01-01 00:00:00

  • ETS sites in the promoters of the matrix metalloproteinases collagenase (MMP-1) and stromelysin (MMP-3) are auxiliary elements that regulate basal and phorbol-induced transcription.

    abstract::The matrix metalloproteinases collagenase (MMP-1) and stromelysin (MMP-3) are often coordinately expressed, and their promoters contain similar regulatory elements, including an AP-1 site at about -70. There are, however, additional sequences including an adjacent ETS site at about -90 in both promoters, and a NIP (nu...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: White LA,Maute C,Brinckerhoff CE

    更新日期:1997-01-01 00:00:00

  • Joint immobilization induced hypoxic and inflammatory conditions in rat knee joints.

    abstract::The purpose of this study was to examine the hypoxic and inflammatory conditions after immobilization in the joint capsule of rat knees. The unilateral knee joints of adult male rats were immobilized with an internal fixator (Im group) for 1 day, 3 days, and 1, 2, 4, 8, and 16 weeks. Sham-operated animals had holes dr...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Yabe Y,Hagiwara Y,Suda H,Ando A,Onoda Y,Tsuchiya M,Hatori K,Itoi E

    更新日期:2013-01-01 00:00:00

  • Orthotic management for children with osteogenesis imperfecta.

    abstract::Fitting children and infants who have osteogenesis imperfecta (OI) with braces has posed substantial problems of implementation and patient management For the past twelve years bracing has been an important component of a patient research and management program conducted at the National Institutes of Health. By using ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Weintrob JC

    更新日期:1995-01-01 00:00:00

  • Identification of genes required for the spontaneous repair of partial-thickness cartilage defects in immature rats.

    abstract:PURPOSE:Our previous study showed that partial-thickness articular cartilage defects (PTCDs) created in immature rats spontaneously healed to resemble normal hyaline cartilage, but that of mature rats did not. To identify molecules involved in the spontaneous cartilage repair observed in this model, gene expression was...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Ikegawa N,Sasho T,Yamaguchi S,Saito M,Akagi R,Muramatsu Y,Akatsu Y,Fukawa T,Nakagawa K,Nakajima A,Suzuki T,Takahashi K

    更新日期:2016-05-01 00:00:00

  • In vivo expression of classic PKC isoforms in the rat dental follicle as related to tooth eruption.

    abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Yao S,Wise G

    更新日期:2004-01-01 00:00:00

  • Glycosaminoglycans in localized scleroderma (morphoea).

    abstract::The composition of glycosaminoglycans (GAGs) was analyzed in skin samples of eight patients suffering from localized scleroderma, i.e., three having generalized morphoea and five localized morphoea plaques. From each patients, biopsies were obtained from sclerotic and perilesional areas, and from clinically uninvolved...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Møller R,Serup J,Ammitzbøll T

    更新日期:1985-01-01 00:00:00

  • Transforming growth factor beta: an autocrine regulator of chondrocytes.

    abstract::Transforming growth factor beta (TGF-beta) is a ubiquitous regulator of cellular growth and differentiation. The present study investigated the effects of TGF-beta on chick growth plate chondrocyte proliferation, matrix synthesis, and alkaline phosphatase activity in short term cultures. TGF-beta markedly stimulated D...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Rosier RN,O'Keefe RJ,Crabb ID,Puzas JE

    更新日期:1989-01-01 00:00:00

  • Interleukin-1 receptor antagonist modulates inflammation and scarring after ligament injury.

    abstract::Ligaments have limited regenerative potential and as a consequence, repair is protracted and results in a mechanically inferior tissue more scar-like than native ligament. We previously reported that a single injection of interleukin-1 receptor antagonist (IL-1Ra) delivered at the time of injury, decreased the number ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Chamberlain CS,Leiferman EM,Frisch KE,Duenwald-Kuehl SE,Brickson SL,Murphy WL,Baer GS,Vanderby R

    更新日期:2014-06-01 00:00:00