Increased elastin mRNA levels associated with surgically induced intimal injury.


:Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative recovery, tissue samples were obtained for histopathology, electron microscopy, RNA extraction and mRNA quantitation. Using a series of mammalian cDNA clones and the procedure of slot blot hybridization, we have shown that elastin and types I and III procollagen mRNA levels rose significantly during the postoperative period following vascular distention. The increase correlated with an increase in the cell mass present in both the venous and arterial intimal layers. Changes in gamma-actin mRNA levels were also associated with this rapid proliferative response but in arterial tissue only.


Connect Tissue Res


Boyd CD,Kniep AC,Pierce RA,Deak SB,Karboski C,Miller DC,Parker MI,Mackenzie JW,Rosenbloom J,Scott GE




Has Abstract


1988-01-01 00:00:00












  • In-vitro culture of human chondrocytes from adult subjects.

    abstract::An agarose gel matrix was utilized to grow chondrocytes from human donors of various ages in cell culture. The chondrocytes produced the pericellular matrix characteristic for such cells and synthesized collagen type II as well as glyco-saminoglycans. The latter exhibit the typical distribution pattern of the respecti...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Delbrück A,Dresow B,Gurr E,Reale E,Schröder H

    更新日期:1986-01-01 00:00:00

  • Normalization of the tumor microenvironment: evidence for tissue inhibitor of metalloproteinase-2 as a cancer therapeutic.

    abstract::Matrix metalloproteinases (MMPs) are members of the Metzincin family of proteases responsible for degrading the extracellular matrix (ECM). In early studies, MMP degradation of the sub-epithelial basement membrane was thought to be tumor cell autonomous and contribute to the invasive behavior of malignant cells. It is...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审


    authors: Stetler-Stevenson WG,Gavil NV

    更新日期:2014-01-01 00:00:00

  • Characterization of a porcine amelogenin preparation, EMDOGAIN, a biological treatment for periodontal disease.

    abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Maycock J,Wood SR,Brookes SJ,Shore RC,Robinson C,Kirkham J

    更新日期:2002-01-01 00:00:00

  • Design and analysis of braid-twist collagen scaffolds.

    abstract::Collagen type I fiber-based scaffolds for anterior cruciate ligament (ACL) replacement were evaluated for their mechanical properties and their ability to promote cellular proliferation. Prior to scaffold formation, two crosslinking methods were investigated on individual reconstituted collagen type I fibers, ultravio...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Walters VI,Kwansa AL,Freeman JW

    更新日期:2012-01-01 00:00:00

  • Arthrofibrosis and large joint scarring.

    abstract::Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are bei...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审


    authors: Chen AF,Lee YS,Seidl AJ,Abboud JA

    更新日期:2019-01-01 00:00:00

  • Geometry of artificial ECM: sizes of pores controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis.

    abstract::This study aims to elucidate the feasible geometry of the scaffolds in bone and periodontal tissue engineering. Several biomaterials with different geometries are compared in terms of their patterns of ectopic BMP-induced chondrogenesis and osteogenesis. The materials include a honeycomb-shaped hydroxyapatite (HCHAP) ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Kuboki Y,Jin Q,Kikuchi M,Mamood J,Takita H

    更新日期:2002-01-01 00:00:00

  • Tissue engineered cartilage integration to live and devitalized cartilage: a study by reflectance mode confocal microscopy and standard histology.

    abstract::This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups wer...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Peretti GM,Campo-Ruiz V,Gonzalez S,Randolph MA,Wei Xu J,Morse KR,Roses RE,Yaremchuk MJ

    更新日期:2006-01-01 00:00:00

  • Association of the C-propeptide of type II collagen with mineralization of embryonic chick long bone and sternal development.

    abstract::Several proteins may play a role in bone formation. The C-propeptide of type II collagen is intimately associated with endochondral bone formation in bovine growth plate. We have used an antibody against this peptide to determine its immunofluorescent distribution in early stages of embryonic chick limb development wi...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Kujawa MJ,Weitzhandler M,Poole AR,Rosenberg L,Caplan AI

    更新日期:1989-01-01 00:00:00

  • JNK MAPK is involved in BMP-2-induced odontoblastic differentiation of human dental pulp cells.

    abstract::Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Qin W,Liu P,Zhang R,Huang S,Gao X,Song Z,Wang R,Chen L,Guo B,Lin Z

    更新日期:2014-06-01 00:00:00

  • Induced hypothyroidism alters articular cartilage in skeletally immature miniature swine.

    abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Bundy JA,Yang JT,Morscher MA,Steiner RP,Adamczyk MJ,Weiner DS,Jacquet RD,Landis WJ

    更新日期:2020-12-11 00:00:00

  • Enamel specific protein kinases and state of phosphorylation of purified amelogenins.

    abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Salih E,Huang JC,Strawich E,Gouverneur M,Glimcher MJ

    更新日期:1998-01-01 00:00:00

  • RANKL expression of primary osteoblasts is enhanced by an IL-17-mediated JAK2/STAT3 pathway through autophagy suppression.

    abstract::Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Wang Z,Wei Y,Lei L,Zhong J,Shen Y,Tan J,Xia M,Wu Y,Sun W,Chen L

    更新日期:2020-05-05 00:00:00

  • The physical properties of elastic fibers and elastolysis.

    abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Hall DA

    更新日期:1976-01-01 00:00:00

  • Distributions of types I, II and III collagen by region in the human supraspinatus tendon.

    abstract::The mechanical properties of the human supraspinatus tendon (SST) are highly heterogeneous and may reflect an important adaptive response to its complex, multiaxial loading environment. However, these functional properties are associated with a location-dependent structure and composition that have not been fully eluc...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Buckley MR,Evans EB,Matuszewski PE,Chen YL,Satchel LN,Elliott DM,Soslowsky LJ,Dodge GR

    更新日期:2013-01-01 00:00:00

  • Insulin and thyroid hormones stimulate matrix metabolism in primary cultures of articular chondrocytes from young rabbits independently and in combination.

    abstract::These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Glade MJ,Kanwar YS,Stern PH

    更新日期:1994-01-01 00:00:00

  • Comparison of the thermal stabilities and solubilities of human hypertrophic scar, normal scar and normal skin collagens.

    abstract::The thermal stability and solubility profiles of human hypertrophic scar collagen have been measured and compared with those of the collagens present in human normal scar, human normal skin and a lathyritic dermal tissue. The thermal stability profiles of the hypertrophic scar collagens were found to be very similar t...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Snowden JM,Swann DA

    更新日期:1984-01-01 00:00:00

  • Biosynthesis of sulfated proteoglycan in vitro by cells derived from human osteochondrophytic spurs of the femoral head.

    abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Malemud CJ,Moskowitz RW,Goldberg VM

    更新日期:1984-01-01 00:00:00

  • An address to young research workers: Inconsequences and blindness to the facts and unpublished observations.

    abstract::The critical remembrance of my research activity displays aspects of inconsequence and mental rigidity. Particular probative examples are called to mind. Unpublished observations concerning immortalized dental cells are summarized. As a consequence, if I could start again, I would improve several aspects of my behavio...

    journal_title:Connective tissue research



    authors: Ruch JV

    更新日期:2002-01-01 00:00:00

  • Stimulation of small proteoglycan synthesis by the hyaluronan synthesis inhibitor 4-methylumbelliferone in human skin fibroblasts.

    abstract::Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Funahashi M,Nakamura T,Kakizaki I,Mizunuma H,Endo M

    更新日期:2009-01-01 00:00:00

  • Hypermineralized whale rostrum as the exemplar for bone mineral.

    abstract::Although bone is a nanocomposite of mineral and collagen, mineral has been the more elusive component of study. A standard for bone mineral is clearly needed. We hypothesized that the most natural, least-processed bone mineral could be retrieved from the most highly mineralized bone. We therefore studied the rostrum o...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Li Z,Pasteris JD,Novack D

    更新日期:2013-01-01 00:00:00

  • Depolymerization and de-N-acetylation of glycosaminoglycuronans by the action of alkali in the presence of sodium borohydride.

    abstract::Depolymerization of Ch-4-S, Ch-6-S, HA and HP was observed by gel filtration after treatment with 2-10 N NaOH or Ba(OH)2 in the presence of NaBH4 at 80-83 degrees for 1-5 hr. Depolymerized products were isolated after these treatments. 4-Deoxy-alpha-L-threo-4-enohexopyranosyluronic acid residue as the nonreducing end ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Hirano S,Ishigami M,Koga Y

    更新日期:1975-01-01 00:00:00

  • Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane.

    abstract::Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to the failure. Substance P (SP) immunoreactive nerve fibers have been detected in the periprosthetic membrane of aseptic loose hip prostheses. We isolat...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Qian Y,Zhang XL,Zeng BF,Jiang Y,Shen H,Wang Q

    更新日期:2013-01-01 00:00:00

  • Hyaluronic acid promotes proliferation and migration of human meniscus cells via a CD44-dependent mechanism.

    abstract:PURPOSE:Treatment of meniscal injury is important for osteoarthritis (OA) prevention. Meniscus cells are divided between inner and outer cells, which have different characteristics and vascularity. We evaluated the effects of hyaluronic acid (HA) on the proliferation and migration of human inner and outer meniscus cell...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Murakami T,Otsuki S,Okamoto Y,Nakagawa K,Wakama H,Okuno N,Neo M

    更新日期:2019-03-01 00:00:00

  • Downregulation of decorin and transforming growth factor-beta1 by decorin gene suppression in tendinocytes.

    abstract::Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Hosaka Y,Kirisawa R,Mafune N,Takehana K

    更新日期:2005-01-01 00:00:00

  • Therapeutic ionizing radiation induced bone loss: a review of in vivo and in vitro findings.

    abstract::Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due t...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Zhang J,Qiu X,Xi K,Hu W,Pei H,Nie J,Wang Z,Ding J,Shang P,Li B,Zhou G

    更新日期:2018-11-01 00:00:00

  • Adenovirus-mediated gene transfer of growth and differentiation factor-5 into tenocytes and the healing rat Achilles tendon.

    abstract::Growth and differentiation factor-5 (GDF-5) is known to induce tendon tissue and stimulate tendon healing. The hypothesis was that adenoviral GDF-5 transfer leads to transitory transgene expression and improves Achilles tendon healing. In vitro experiments were first performed with rat tenocytes. Transgene expression ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Rickert M,Wang H,Wieloch P,Lorenz H,Steck E,Sabo D,Richter W

    更新日期:2005-01-01 00:00:00

  • Healing of biodegradable vascular prosthesis. Incorporation of 3H-valine into proteins in the subendothelial scar and host intima-media of rat aorta.

    abstract::Heparin treated and aldehyde crosslinked rat aorta segments were implanted in infrarenal aorta of homologous rats. One year following aortic replacement, the subendothelial scar and the prosthetic remnants were excised. The scar and the host intima-media were incubated with 3H-valine for 4 h and extracted with 5 M gua...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Moczar M,Bessou JP,Loisance D

    更新日期:1983-01-01 00:00:00

  • Ovalbumin is a component of the chicken eggshell matrix.

    abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Hincke MT

    更新日期:1995-01-01 00:00:00

  • Expression of the elastin promoter in novel tissue sites in transgenic mouse embryos.

    abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Lakkakorpi J,Li K,Decker S,Korkeela E,Piddington R,Abrams W,Bashir M,Uitto J,Rosenbloom J

    更新日期:1999-01-01 00:00:00

  • Alterations of elastin fibrogenesis by inhibition of the formation of desmosine crosslinks. Comparison between the effect of beta-aminopropionitrile (beta-APN) and penicillamine.

    abstract::Experimental lathyrism was induced by feeding newborn chicks a diet containing 0.2 and 0.4% DL-Penicillamine, with or without CuSO4 (10 mg/Kg diet) and Vitamin B6 (100 mg/Kg diet), or 0.015 and 0.1% beta-aminopropionitrile fumarate (beta-APN). After 7, 15, 25 and 55 days of treatment the animals were killed, the aorta...

    journal_title:Connective tissue research

    pub_type: 杂志文章


    authors: Ronchetti IP,Contri MB,Fornieri C,Quaglino D Jr,Mori G

    更新日期:1985-01-01 00:00:00