Abstract:
:??Although injuries to the medial collateral ligament (MCL) can heal functionally without surgical intervention, the collagen fibers in the healing tissue remain compromised. The molecular basis for this poor healing potential was investigated by examining extracellular matrix-modifying molecules such as bone morphogenetic protein 1 (BMP-1), procollagen C proteinase enhancer (PCOLCE), lysyl oxidase (LOX), and transforming growth factor beta 1 (TGF-β1) involved in collagen fibrillogenesis during normal early postnatal ligament maturation and at comparable intervals after MCL injury. Samples of midsections of rabbit MCLs were collected from 3-, 6-, 14-, and 52-week-old normal animals and at 3, 6, and 14 weeks postinjury. Harvested midsubstance tissues were analyzed for collagen fibril diameter by transmission electron microscopy (TEM), and mRNA levels were assessed by reverse transcription-polymerase chain reaction (RT-PCR). Results showed different patterns of expression between normal MCL maturation and during scar maturation. BMP-1 and PCOLCE mRNA levels were upregulated in the 3?14-week period during maturation of normal ligaments but decreased at skeletal maturity. The scar tissue exhibited a 3.5-fold increase in PCOLCE mRNA levels during the early healing phase, but these decreased with time. After injury, BMP-1 mRNA levels in scars were low and did not change during healing. Both LOX and TGF-β1 mRNA levels were low during normal MCL development compared with levels at maturity and exhibited elevated mRNA levels during early healing that decreased with time postinjury. These results suggest that gene expression in scars during MCL healing does not recapitulate expression in normal ligament fibroblasts during maturation.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Achari Y,Chin JW,Heard BJ,Rattner JB,Shrive NG,Frank CB,Hart DAdoi
10.3109/03008207.2010.524719subject
Has Abstractpub_date
2011-01-01 00:00:00pages
301-12issue
4eissn
0300-8207issn
1607-8438journal_volume
52pub_type
杂志文章abstract::Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152629
更新日期:1978-01-01 00:00:00
abstract::The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609017468
更新日期:1986-01-01 00:00:00
abstract:AIMS:The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate th...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1235566
更新日期:2017-09-01 00:00:00
abstract::The paper reviews the changes in ameloblast ultrastructure, concomitant with the changes in its functions across the major stages of amelogenesis. It describes the mechanisms associated with the major events in biosynthesis and degradation of the major enamel proteins (amelogenins and tuftelin/enamelins) and with the ...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509013710
更新日期:1995-01-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::Bovine articular chondrocytes cultured in agarose gel comprise a heterogeneous population when judged by morphological and histochemical criteria. The purpose of the present experiments was to compare, under the same conditions of culture, sub-populations of chondrocytes derived from different depths of articular cart...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809016808
更新日期:1988-01-01 00:00:00
abstract::Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1759562
更新日期:2020-05-05 00:00:00
abstract::Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809002438
更新日期:1998-01-01 00:00:00
abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409013694
更新日期:1984-01-01 00:00:00
abstract::The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109016840
更新日期:2001-01-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.882331
更新日期:2014-06-01 00:00:00
abstract::Size measurements of dispersed rat bone crystals show that with age a greater proportion exceeds 400 A in length. The surface fractures of more heavily mineralized bones tend to be smooth and stepped, whereas those of less mineralized bones are fibrous. These observations combined with information reported elsewhere o...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909050015
更新日期:1989-01-01 00:00:00
abstract::To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of la...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709001994
更新日期:1987-01-01 00:00:00
abstract::The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023927
更新日期:1998-01-01 00:00:00
abstract::Many organisms from a wide variety of taxa produce amorphous calcium carbonate (ACC), despite the fact that it is inherently unstable and relatively soluble in its pure state. These properties also make it difficult to detect and characterize ACC. Raman spectroscopy is a particularly useful method for investigating AC...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809008059
更新日期:1988-01-01 00:00:00
abstract::DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152225
更新日期:1976-01-01 00:00:00
abstract::An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pe...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152162
更新日期:1991-01-01 00:00:00
abstract::The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152378
更新日期:1981-01-01 00:00:00
abstract::A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109029158
更新日期:1991-01-01 00:00:00
abstract::Experimental lathyrism was induced by feeding newborn chicks a diet containing 0.2 and 0.4% DL-Penicillamine, with or without CuSO4 (10 mg/Kg diet) and Vitamin B6 (100 mg/Kg diet), or 0.015 and 0.1% beta-aminopropionitrile fumarate (beta-APN). After 7, 15, 25 and 55 days of treatment the animals were killed, the aorta...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509015021
更新日期:1985-01-01 00:00:00
abstract::X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109160244
更新日期:1981-01-01 00:00:00
abstract::The menisci are intricately organized structures that perform many tasks in the knee. We review their structure and function and introduce new data about their tibial and femoral surfaces. As the femur and tibia approach each other when the knee is bearing load, circumferential tension develops in the menisci, enablin...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2017.1303489
更新日期:2017-01-01 00:00:00
abstract::Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017041
更新日期:1998-01-01 00:00:00
abstract:PURPOSE:Orthodontic tooth movement occurs during the bone remodeling induced by therapeutic mechanical strain. It is important to investigate the relation between the strength of mechanical stress and bone formation activity. The aim of this study was to determine the effect of high-magnitude mechanical strain on bone ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2015.1040878
更新日期:2015-01-01 00:00:00
abstract::Laminin promotes adhesion of various cell types via multiple interactions with cell surface components. We have used a laminin domain involved in adhesion of melanoma cells, peptide F9 (Charonis et al., J. Cell Biol. 107:1253 [( 1988]), to examine its specific interaction with cell surface components. Cells were surfa...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152435
更新日期:1991-01-01 00:00:00
abstract::Glycosaminoglycan synthesis by cells cultured from nodule or band tissues of patients with Dupuytren's disease was compared with that of skin fibroblasts cultured from uninvolved areas of the palm. No differences were observed in culture which could account either for the abnormal glycosaminoglycan content or the incr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160272
更新日期:1982-01-01 00:00:00
abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1485666
更新日期:2018-09-01 00:00:00
abstract::Proteoglycans and hyaluronic acid synthesized by human skin fibroblasts in culture were characterized, and the effect of thyroid hormone deficiency was examined. The fibroblasts in culture synthesize hyaluronic acid, dermatan sulfate (DS) proteoglycans and heparan sulfate (HS) proteoglycans. Hyaluronic acid is almost ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809015024
更新日期:1988-01-01 00:00:00