Detection of tubulointerstitial nephritis antigen (TIN-ag) in Lewis rat.

Abstract:

:Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TIN-ag, with the site of most prominent expression the kidney tubular basement membrane. However, Lewis rat does not demonstrate any immunoreactivity for TIN-ag and does not develop tubuloinsterstitial nephritis after injection of tubular basement membrane material. As TIN-ag would appear to be a molecule of biological significance, experiments were designed to explore the presence or absence of this macromolecule in the Lewis rat model. Southern blotting of Lewis rat genomic DNA revealed the presence of gene sequences corresponding to TIN-ag. RTPCR analysis of Lewis rat kidney cortex total RNA illustrated the expression of a TIN-ag gene product. Western blotting demonstrated the presence of TIN-ag protein forms in kidney cortical homogenates of Lewis rat. The data suggest either extensive epitope masking or expression polymorphism of TIN-ag in the Lewis rat.

journal_name

Connect Tissue Res

authors

Nelson TR,Butkowski RJ,Michael AF,Charonis AS

doi

10.3109/03008209709160222

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

223-9

issue

3

eissn

0300-8207

issn

1607-8438

journal_volume

36

pub_type

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