Abstract:
:During cloning of the rat incisor phosphophoryn gene, several clones were identified with a PP antibody. One of the clones (2PP) was shown to encode a PP denoted as dentin matrix protein 2, DMP2. We have now sequenced another clone, which appears to be bifunctional, or at minimum, has two distinct domains. The 5' region encodes for dentin sialoprotein (DSP), while the 3' region encodes a small or "mini" PP. There is no stop codon between these domains. The whole gene has been named Dmp3, in keeping with the current nomenclature adopted in our laboratory. The previously reported Dmp2 gene does not encode a DSP domain but has a 114 amino acid carboxy terminal sequence identical except for a single residue with that of the DMP3. Thus, Dmp2, Dmp3 and their corresponding proteins, probably represent related members of a multigene family. There is no evidence for differential splicing. Since the DSP isolated from dentin does not carry the mini-PP domain, it must be cleaved postranslationally from DMP3.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
George A,Srinivasan RThotakura SR,Liu K,Veis Adoi
10.3109/03008209909005277keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
49-57issue
1eissn
0300-8207issn
1607-8438journal_volume
40pub_type
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journal_title:Connective tissue research
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abstract::Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
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abstract::Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum fr...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title:Connective tissue research
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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更新日期:2005-01-01 00:00:00
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更新日期:2018-01-01 00:00:00
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更新日期:2021-03-01 00:00:00
abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...
journal_title:Connective tissue research
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更新日期:2002-01-01 00:00:00
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更新日期:2014-08-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010825
更新日期:1995-01-01 00:00:00
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doi:10.1080/03008207.2018.1483356
更新日期:2019-03-01 00:00:00
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journal_title:Connective tissue research
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更新日期:1988-01-01 00:00:00
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journal_title:Connective tissue research
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journal_title:Connective tissue research
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
abstract::Ultrasound is a therapeutic agent commonly used to treat sports-related tendinopathy. Tendon healing requires tendon cells migration to the repair site, followed by the proliferation and synthesis of extracellular matrix. This study was designed to determine the effect of ultrasound on migration of tendon cells intrin...
journal_title:Connective tissue research
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更新日期:2008-01-01 00:00:00
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journal_title:Connective tissue research
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更新日期:1992-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章,评审
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更新日期:1989-01-01 00:00:00
abstract::Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...
journal_title:Connective tissue research
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doi:10.3109/03008209809002438
更新日期:1998-01-01 00:00:00
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更新日期:2020-05-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00