Abstract:
:Osteoarthritis (OA) is typically managed in late stages by replacement of the articular cartilage surface with a prosthesis as an effective, though undesirable outcome. As an alternative, hydrogel implants or growth factor treatments are currently of great interest in the tissue engineering community, and scaffold materials are often designed to emulate the mechanical and chemical composition of mature extracellular matrix (ECM) tissue. However, scaffolds frequently fail to capture the structure and organization of cartilage. Additionally, many current scaffold designs do not mimic processes by which structurally sound cartilage is formed during musculoskeletal development. The objective of this review is to highlight methods that investigate cartilage ontogenesis with native and model systems in the context of regenerative medicine. Specific emphasis is placed on the use of cartilage explant cultures that provide a physiologically relevant microenvironment to study tissue assembly and development. Ex vivo cartilage has proven to be a cost-effective and accessible model system that allows researchers to control the culture conditions and stimuli and perform proteomics and imaging studies that are not easily possible using in vivo experiments, while preserving native cell-matrix interactions. We anticipate our review will promote a developmental biology approach using explanted tissues to guide cartilage tissue engineering and inform new treatment methods for OA and joint damage.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
McCreery KP,Calve S,Neu CPdoi
10.1080/03008207.2019.1698556subject
Has Abstractpub_date
2020-05-01 00:00:00pages
278-291issue
3-4eissn
0300-8207issn
1607-8438journal_volume
61pub_type
杂志文章abstract::The critical remembrance of my research activity displays aspects of inconsequence and mental rigidity. Particular probative examples are called to mind. Unpublished observations concerning immortalized dental cells are summarized. As a consequence, if I could start again, I would improve several aspects of my behavio...
journal_title:Connective tissue research
pub_type:
doi:10.1080/03008200290001195
更新日期:2002-01-01 00:00:00
abstract::This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009152156
更新日期:1990-01-01 00:00:00
abstract::Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum fr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009812
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1276177
更新日期:2017-01-01 00:00:00
abstract::Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923851
更新日期:2014-08-01 00:00:00
abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010814
更新日期:1995-01-01 00:00:00
abstract::The ultrastructural features of the cells and of the oxytalan fibers within the periodontal ligaments of aged rats (2 year old) were quantified and compared with data for tissues obtained from younger animals (8 week old). Sections of the mid-root regions of the mandibular first molars were prepared for examination by...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509016978
更新日期:1995-01-01 00:00:00
abstract::Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802143141
更新日期:2008-01-01 00:00:00
abstract::Part of the hyaluronic acid (HA) synthesized in peripheral tissues enters the blood circulation through the lymph. It is rapidly taken up by the endothelial cells in the liver (half-life in blood is 2.5-5.5 minutes) and degraded. Pure primary cultures of liver endothelial cells were obtained by a newly developed techn...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609001971
更新日期:1986-01-01 00:00:00
abstract::Eruption requires synchrony of the tooth with the surrounding tissues, particularly the bone. One important step during eruption is remodelling of the alveolar bone at the base of the tooth and along the roots. Expression of BMP6 was reported to be increased in the basal half of the dental follicle prior to eruption a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951441
更新日期:2014-10-01 00:00:00
abstract::A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013726
更新日期:1995-01-01 00:00:00
abstract::To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109005649
更新日期:2001-01-01 00:00:00
abstract:OVERVIEW:Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance; however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of PO...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1306060
更新日期:2018-03-01 00:00:00
abstract::The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023927
更新日期:1998-01-01 00:00:00
abstract::This investigation is devoted to the study of the viscoelastic behavior of human abdominal fascia from the umbilical region. Seventeen samples 10 mm wide and up to 70 mm long were cut along the primary fiber direction (group FL) or perpendicular to it (group FT) and subjected to relaxation tests. The viscoelastic resp...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2011.604452
更新日期:2012-01-01 00:00:00
abstract::The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152627
更新日期:1978-01-01 00:00:00
abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1195826
更新日期:2016-09-01 00:00:00
abstract::Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000565
更新日期:2002-01-01 00:00:00
abstract::Positive effects on the tensile characteristics of swine digital extensors were found following twelve months of exercise training. Compared to sedentary controls, the tendons from the exercised animals became stronger as a material and exhibited hypertrophy. These biomechanical results were supported by biochemical a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208009152109
更新日期:1980-01-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::STR/ort mice develop a naturally occurring osteoarthritis (OA) of the knee joints. However, the evaluation of early OA changes has been difficult due to variability caused by gender, individual differences, and differences between the right and left lower limbs. The objective of this study was to analyze the variabili...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200902836065
更新日期:2009-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::Two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either HaeIII/AluI or Sau3A and contained in lambda Charon 4A or EMBL3, respectively. The 6 kb of DNA comprising the most 3' portion of the gene were sequenced demonstrati...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709006976
更新日期:1987-01-01 00:00:00
abstract::Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.775258
更新日期:2013-01-01 00:00:00
abstract::The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152378
更新日期:1981-01-01 00:00:00
abstract::To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of la...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709001994
更新日期:1987-01-01 00:00:00
abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490882628
更新日期:2004-01-01 00:00:00
abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2012.756871
更新日期:2013-01-01 00:00:00
abstract::The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802171159
更新日期:2008-01-01 00:00:00
abstract::Although fibronectin (FN) modified by advanced glycation end products (AGEs) has been shown to contribute to the development of diabetic vascular complications through its reduced adhesive activity to vascular cells, little is known about changes in the cell binding domain of AGE-modified FN. Here we examined the mech...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009005291
更新日期:2000-01-01 00:00:00