Tissue engineered cartilage integration to live and devitalized cartilage: a study by reflectance mode confocal microscopy and standard histology.

Abstract:

:This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups were prepared: in groups 1 and 2, the cell-hydrogel composite was placed between two live or between two devitalized cartilage discs, respectively; in groups 3 and 4, acellular fibrin glue was placed between two live or between two devitalized cartilage discs, respectively. Samples were implanted in the back of nude mice and analyzed after 2, 5, and 8 weeks. Results showed that engineered cartilage seems to grow more homogenously when the cell-seeded gel was placed between devitalized cartilages than when it was placed between live cartilage matrices. Confocal microscopy provides valuable information on the integration of tissue-engineered cartilage with native tissue and could be useful for nondestructive imaging in vivo.

journal_name

Connect Tissue Res

authors

Peretti GM,Campo-Ruiz V,Gonzalez S,Randolph MA,Wei Xu J,Morse KR,Roses RE,Yaremchuk MJ

doi

10.1080/03008200600809935

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

190-9

issue

4

eissn

0300-8207

issn

1607-8438

pii

V8738742R0768V42

journal_volume

47

pub_type

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