Abstract：
:Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum free conditions. Using a combination of rapid column chromatography steps, approximately 10 milligrams of prostromelysin and 5 milligrams of procollagenase were purified from 1 liter of conditioned media. Prostromelysin electrophoresed as a doublet with molecular weights of 55,57 kD, whereas, procollagenase migrated with slightly lower molecular weights of 52, 54 kD. Both proenzymes were treated with trypsin or aminophenylmercuric acetate to generate active species. The molecular weights of the active enzymes were approximately 10 kD smaller than the proenzymes. Active enzymes were inhibited by metal chelators and the natural metalloproteinase inhibitor, tissue inhibitor of metalloproteinase (TIMP), but not by the serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF). Activated stromelysin degraded a number of substrates including transferrin, proteoglycan monomer, proteoglycan aggregated with hyaluronic acid, and substance P. By contrast, collagenase degraded interstitial type I collagen and the peptide thioester, Ac-Pro-Leu-Gly-SCH(iBu)Co-Leu-GlyOEt. Identity of both enzymes were confirmed by amino-terminal protein sequence analysis as well as by immunoblot analysis using monoclonal antibodies.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Lark MW,Walakovits LA,Shah TK,Vanmiddlesworth J,Cameron PM,Lin TYdoi
10.3109/03008209009009812subject
Has Abstractpub_date
1990-01-01 00:00:00pages
49-65issue
1eissn
0300-8207issn
1607-8438journal_volume
25pub_type
杂志文章abstract：:Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209309016835
更新日期：1993-01-01 00:00:00
abstract：:Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collage...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200601143508
更新日期：2007-01-01 00:00:00
abstract：:To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model. Specifically, immunohis...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209809021505
更新日期：1998-01-01 00:00:00
abstract：:Objective: Aseptic loosening is a major problem in total joint replacement. Implant wear debris provokes a foreign body host response and activates cells to produce a variety of mediators and ROS, leading to periprosthetic osteolysis. Elevated ROS levels can harm proteasome function. Proteasome inhibitors have been re...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2019.1601186
更新日期：2019-11-01 00:00:00
abstract：:The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipi...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208109152131
更新日期：1981-01-01 00:00:00
abstract：:Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2019.1651848
更新日期：2021-03-01 00:00:00
abstract：UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2014.923875
更新日期：2014-08-01 00:00:00
abstract：:Genetic diseases affecting tooth structure have been classified by the tissue affected enamel versus dentin, and their pattern of inheritance autosomal dominant, autosomal recessive, or X-linked. Advances in molecular genetics and the Human Genome Project have provided substantial progress regarding the identification...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to the failure. Substance P (SP) immunoreactive nerve fibers have been detected in the periprosthetic membrane of aseptic loose hip prostheses. We isolat...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2013.823953
更新日期：2013-01-01 00:00:00
abstract：:Vascular smooth muscle cells (VSMC)s are characterized by their acute growth inhibition by heparin and heparan sulfates; however, recently the isolation of VSMCs which display greatly diminished sensitivity to the antiproliferative action of heparin have been reported. These heparin resistant (HR) VSMCs have been deri...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：10.3109/03008209809028903
更新日期：1998-01-01 00:00:00
abstract：:The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208309004860
更新日期：1983-01-01 00:00:00
abstract：:Purpose: Complications including arthrofibrosis have been reported after anterior cruciate ligament reconstruction (ACLR) even under accelerated rehabilitation. To overcome this, we developed an animal model of ACLR-induced arthrofibrosis without immobilization.Materials and Methods: Thirteen male Wistar rats were div...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2019.1693548
更新日期：2019-11-26 00:00:00
abstract：:Effects of applied hydrostatic pressure on transmembrane potentials were investigated in sheep articular chondrocytes and human skin fibroblasts in non-confluent monolayer cultures. Resting potentials in chondrocytes (about -12 mv) and in fibroblasts (about -15 mV) were increased and decreased respectively by over 40%...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209209014227
更新日期：1992-01-01 00:00:00
abstract：:The in-vitro fluoride treatment technique has been introduced to investigate the composite behavior of bone tissue. Bone tissue with different mechanical properties can be obtained by varying the concentration, pH and immersion time in fluoride ion solutions. The chemical and physical changes in intact pieces of bone ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2014.951440
更新日期：2014-10-01 00:00:00
abstract：:A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208109152380
更新日期：1981-01-01 00:00:00
abstract：PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1485666
更新日期：2018-09-01 00:00:00
abstract：:Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208809019069
更新日期：1988-01-01 00:00:00
abstract：:Since cartilage is mainly an avascular tissue, chondrocytes exist in a low-level oxygen environment in vivo. In the present study, we investigated the effect of oxygen tension (20%, 5% and 1% gas phase oxygen concentrations) over a 20-day period on the extracellular matrix accumulation of bovine articular chondrocytes...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008200109014251
更新日期：2001-10-01 00:00:00
abstract：:Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207809152287
更新日期：1978-01-01 00:00:00
abstract：:Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2010.546536
更新日期：2011-10-01 00:00:00
abstract：:Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209609028895
更新日期：1996-01-01 00:00:00
abstract：:The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209809023927
更新日期：1998-01-01 00:00:00
abstract：:Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due t...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1439482
更新日期：2018-11-01 00:00:00
abstract：:Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxypro...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208009152117
更新日期：1980-01-01 00:00:00
abstract：:The pharmacokinetics of desmosine following a 5 mg intravenous dose was determined in five sheep. The biological half-life (t 1/2 beta) of desmosine varied between 81-489 min. Urinary recovery of unchanged desmosine indicated that the compound is not extensively metabolized and that the primary route of elimination is...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208409013681
更新日期：1984-01-01 00:00:00
abstract：:Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209809017041
更新日期：1998-01-01 00:00:00
abstract：:By using ultramorphological and biochemical methods, we analyzed the regional differences between the three parts of the equine superficial digital flexor tendon (SDFT), namely, the myotendinous junction (MTJ), middle metacarpal (mM), and osteotendinous junction (OTJ). Cross-sectional images showed unique distribution...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200701692800
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:This study proposes to evaluate, by means of microtomographic analysis, the topical potential of raloxifene combined with BioGran® through the sonochemical method in the repair of critical bone defects in the calvaria of rats. The hypothesis was that the homogenization of Raloxifene to Biogran at the 20% conc...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1430143
更新日期：2018-12-01 00:00:00
