Abstract：
:Vascular smooth muscle cells (VSMC)s are characterized by their acute growth inhibition by heparin and heparan sulfates; however, recently the isolation of VSMCs which display greatly diminished sensitivity to the antiproliferative action of heparin have been reported. These heparin resistant (HR) VSMCs have been derived through multiple passage of normal rat VSMCs in culture media containing high heparin doses, by transformation of VSMCs with oncogene-containing vectors, or have been isolated from vascular tissues of spontaneously hypertensive rats, healthy humans, or humans with restenosis where their presence is not limited to sites of injury. Initial characterizations of HR VSMCs are reviewed, and here we propose a definition of HR VSMCs. To date the mechanisms underlying heparin insensitivity remain elusive. Further study of HR VSMCs may expand our understanding of cell growth regulation by heparin, establish whether HR VSMCs contribute to the reported failure of heparin to combat restenosis in humans, and identify cellular mechanisms driving certain vascular proliferative diseases.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
San Antonio JD,Verrecchio A,Pukac LAdoi
10.3109/03008209809028903subject
Has Abstractpub_date
1998-01-01 00:00:00pages
87-103issue
1-2eissn
0300-8207issn
1607-8438journal_volume
37pub_type
杂志文章,评审abstract：:Currently, the mechanism governing the regeneration of the soft tissue-to-bone interface, such as the transition between the anterior cruciate ligament (ACL) and bone, is not known. Focusing on the ACL-to-bone insertion, this study tests the novel hypothesis that interactions between cells from the ligament (fibroblas...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2016.1230106
更新日期：2016-11-01 00:00:00
abstract：:The menisci are intricately organized structures that perform many tasks in the knee. We review their structure and function and introduce new data about their tibial and femoral surfaces. As the femur and tibia approach each other when the knee is bearing load, circumferential tension develops in the menisci, enablin...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：10.1080/03008207.2017.1303489
更新日期：2017-01-01 00:00:00
abstract：:Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum fr...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209009009812
更新日期：1990-01-01 00:00:00
abstract：:Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progen...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1553960
更新日期：2020-03-01 00:00:00
abstract：:DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207609152225
更新日期：1976-01-01 00:00:00
abstract：:Many organisms from a wide variety of taxa produce amorphous calcium carbonate (ACC), despite the fact that it is inherently unstable and relatively soluble in its pure state. These properties also make it difficult to detect and characterize ACC. Raman spectroscopy is a particularly useful method for investigating AC...
journal_title：Connective tissue research
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2016.1276177
更新日期：2017-01-01 00:00:00
abstract：:These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209409005633
更新日期：1994-01-01 00:00:00
abstract：:Two populations of primary osteoblasts and the cloned murine osteoblast cell line MC3T3 were activated with osteotropic agents and the conditioned media tested for the presence of macrophage colony stimulating factor (M-CSF). Differences in ability of the three populations to secrete the cytokine as well as in the eff...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208909023884
更新日期：1989-01-01 00:00:00
abstract：:Collagen type I fiber-based scaffolds for anterior cruciate ligament (ACL) replacement were evaluated for their mechanical properties and their ability to promote cellular proliferation. Prior to scaffold formation, two crosslinking methods were investigated on individual reconstituted collagen type I fibers, ultravio...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2011.634532
更新日期：2012-01-01 00:00:00
abstract：:Eruption requires synchrony of the tooth with the surrounding tissues, particularly the bone. One important step during eruption is remodelling of the alveolar bone at the base of the tooth and along the roots. Expression of BMP6 was reported to be increased in the basal half of the dental follicle prior to eruption a...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2014.951441
更新日期：2014-10-01 00:00:00
abstract：:Fras1 and the structurally related proteins Frem1, Frem2, and Frem3, comprise a novel family of extracellular matrix proteins, which localize in a similar fashion underneath the lamina densa of epithelial basement membranes. They are involved in the structural adhesion of the skin epithelium to its underlying mesenchy...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200802148025
更新日期：2008-01-01 00:00:00
abstract：:A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208109152380
更新日期：1981-01-01 00:00:00
abstract：PURPOSE:Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mine...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1424149
更新日期：2018-12-01 00:00:00
abstract：:Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxypro...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208009152117
更新日期：1980-01-01 00:00:00
abstract：:This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups wer...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200600809935
更新日期：2006-01-01 00:00:00
abstract：:Osteogenesis imperfecta is a congenital disease commonly characterized by brittle bones and caused by mutations in the genes encoding Type I collagen, the single most abundant protein produced by the body. The oim model has a natural collagen mutation, converting its heterotrimeric structure (two α1 and one α2 chains)...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2014.923860
更新日期：2014-08-01 00:00:00
abstract：:The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209309016835
更新日期：1993-01-01 00:00:00
abstract：:The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：AIM:miR-155 is a pro-inflammatory or anti-inflammatory factor depending on the cell type in which it is expressed. miR-155 controls apoptosis and matrix degradation in nucleus pulposus (NP) cells in vitro. The aim of this study is to explore the effect of miR-155 in vivo and further investigate the mechanism of miR-155...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2018.1483356
更新日期：2019-03-01 00:00:00
abstract：:This study evaluated the effect of short bouts of stretching on the soleus muscle after immobilization, by measuring the birefringence of the intramuscular connective tissue (ICT) and the muscle fiber area. Thirty rats were divided into five groups: the left soleus was immobilized in the shortened position; after immo...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200600995940
更新日期：2006-01-01 00:00:00
abstract：:Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008200390229912
更新日期：2003-01-01 00:00:00
abstract：:In our previous developmental study on skin we reported that correlations existed between the collagen fibril diameter distribution, glycosaminoglycan content and composition, and mechanical role. In this work we present new but related electron microscope data on the ultrastructure of skins from flounder, marlin, cod...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208709006977
更新日期：1987-01-01 00:00:00
abstract：PURPOSE:Orthodontic tooth movement occurs during the bone remodeling induced by therapeutic mechanical strain. It is important to investigate the relation between the strength of mechanical stress and bone formation activity. The aim of this study was to determine the effect of high-magnitude mechanical strain on bone ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2015.1040878
更新日期：2015-01-01 00:00:00
abstract：:Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.1080/03008207.2020.1751140
更新日期：2020-04-20 00:00:00
abstract：:Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008207.2012.756871
更新日期：2013-01-01 00:00:00
abstract：:The experiments described herein tested the effects of CaCl2 and ZnCl2, added at various concentrations in the culture medium, upon the synthesis of collagen and proteoglycan by adult and fetal (articular, epiphyseal and hypertrophic) bovine chondrocytes maintained in high density multilayer cultures. CaCl2 concentrat...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209609000700
更新日期：1996-01-01 00:00:00
abstract：:The effect of intra-bone injection of differentiated rat bone marrow mesenchymal stem cells (BMMSCs) into the femur of osteoporotic female rats was studied. Osteoporosis was induced in Wistar female rats by bilateral ovariectomy. Then, 0.75 million BMMSCs isolated from healthy rats were injected into the femurs of ost...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008201003597049
更新日期：2010-12-01 00:00:00
abstract：:Molecular distributions of the intermolecular cross-links in fetal bovine bone type I collagen fibrils were quantitatively determined and compared with those of periodontal ligament. Results indicated that Hyl and Lys residues in the COOH-terminal nonhelical peptide portions (residues 16C) of both alpha 1 chains were ...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008208909050006
更新日期：1989-01-01 00:00:00
abstract：:Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...
journal_title：Connective tissue research
pub_type： 杂志文章
doi：10.3109/03008209809002438
更新日期：1998-01-01 00:00:00
