Abstract:
:Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progenitor cells (ACPCs), and multipotent mesenchymal stromal cells (MSCs) embedded in these bio-inks.The incorporating of HAMA in gelMA/gellan bio-ink increased filament stability, as measured using a filament collapse assay, but did not influence (zone-specific) chondrogenesis of any of the cell types. Highest chondrogenic potential was observed for MSCs, followed by ACPCs, which displayed relatively high proteoglycan IV mRNA levels. Therefore, two-zone constructs were printed with gelMA/gellan/HAMA containing ACPCs in the superficial region and MSCs in the middle/deep region. Chondrogenic differentiation was confirmed, however, printing influence cellular differentiation.ACPC- and MSC-laden gelMA/gellan/HAMA hydrogels are of interest for the fabrication of cartilage constructs. Nevertheless, this study underscores the need for careful evaluation of the effects of printing on cellular differentiation.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Mouser VHM,Levato R,Mensinga A,Dhert WJA,Gawlitta D,Malda Jdoi
10.1080/03008207.2018.1553960subject
Has Abstractpub_date
2020-03-01 00:00:00pages
137-151issue
2eissn
0300-8207issn
1607-8438journal_volume
61pub_type
杂志文章abstract::Hyaluronate lyase from streptococci group A was purified by chromatography on DEAE-Sephadex A-50 and on Biogel P-150, thereby enriching it about 1,000-fold and separating it into two enzyme fractions with the same amino acid composition. The photooxidation of hyaluronate lyase in the presence of methylene blue results...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207509152171
更新日期:1975-01-01 00:00:00
abstract:OBJECTIVE:This study proposes to evaluate, by means of microtomographic analysis, the topical potential of raloxifene combined with BioGran® through the sonochemical method in the repair of critical bone defects in the calvaria of rats. The hypothesis was that the homogenization of Raloxifene to Biogran at the 20% conc...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1430143
更新日期:2018-12-01 00:00:00
abstract::The matrix metalloproteinases collagenase (MMP-1) and stromelysin (MMP-3) are often coordinately expressed, and their promoters contain similar regulatory elements, including an AP-1 site at about -70. There are, however, additional sequences including an adjacent ETS site at about -90 in both promoters, and a NIP (nu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160231
更新日期:1997-01-01 00:00:00
abstract::This investigation is devoted to the study of the viscoelastic behavior of human abdominal fascia from the umbilical region. Seventeen samples 10 mm wide and up to 70 mm long were cut along the primary fiber direction (group FL) or perpendicular to it (group FT) and subjected to relaxation tests. The viscoelastic resp...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2011.604452
更新日期:2012-01-01 00:00:00
abstract::Molecular distributions of the intermolecular cross-links in fetal bovine bone type I collagen fibrils were quantitatively determined and compared with those of periodontal ligament. Results indicated that Hyl and Lys residues in the COOH-terminal nonhelical peptide portions (residues 16C) of both alpha 1 chains were ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909050006
更新日期:1989-01-01 00:00:00
abstract::A method is described for accurate and reproducible determination of wet and dry weights of small articular cartilage samples. The water content of the articular cartilage samples before and after treatment with selective degradative enzymes and "artificial fibrillation" was determined. It was found that the water con...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160266
更新日期:1982-01-01 00:00:00
abstract:AIMS:The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate th...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1235566
更新日期:2017-09-01 00:00:00
abstract::The present study details structural aspects of the cells of the rat intra-articular disc (IAD) of the temporomandibular joint. At the light and electron microscope level, the outline of the cells varied, although a number appeared oval/rounded. Ultrastructurally, the cells contained moderate amounts of the intracellu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Although bone is a nanocomposite of mineral and collagen, mineral has been the more elusive component of study. A standard for bone mineral is clearly needed. We hypothesized that the most natural, least-processed bone mineral could be retrieved from the most highly mineralized bone. We therefore studied the rostrum o...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.769973
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mine...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1424149
更新日期:2018-12-01 00:00:00
abstract::The mineralized matrices of enamel, cementum, dentin, calcified cartilage and bone are similar in their ability to form a microenvironment that facilitates deposition of hydroxyapatite. However, they are not identical, as witnessed by the nature of apatite crystals that are formed. Enamel is devoid of collagen; and is...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209609029183
更新日期:1996-01-01 00:00:00
abstract::We previously reported that circulating peripheral blood-borne cells (PBCs) contribute to early-phase meniscal reparative change. Because macrophages and myofibroblasts are important contributors of tissue regeneration, we examined their origin and distribution in the reparative meniscus. Reparative menisci were evalu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1284825
更新日期:2017-11-01 00:00:00
abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809008059
更新日期:1988-01-01 00:00:00
abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2012.756871
更新日期:2013-01-01 00:00:00
abstract::Recently our laboratory has reported, in a lacerated flexor tendon model, that the "early turnover" phase of the repair process extends for a longer period of time in vivo than previously documented. The extensive turnover of the collagenous matrix was consistent with the presence of collagenolytic activity in repairi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909103903
更新日期:1989-01-01 00:00:00
abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000754
更新日期:2002-01-01 00:00:00
abstract::The fibrous collagens form the structural basis of all mammalian connective tissues, including the vasculature, dermis, bones, tendons, cartilage, and those tissues that support organs such as the heart, kidneys, liver, and lungs. The helical structure of collagen has been extensively studied but in addition to its he...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2010.511353
更新日期:2011-02-01 00:00:00
abstract::Laminin promotes adhesion of various cell types via multiple interactions with cell surface components. We have used a laminin domain involved in adhesion of melanoma cells, peptide F9 (Charonis et al., J. Cell Biol. 107:1253 [( 1988]), to examine its specific interaction with cell surface components. Cells were surfa...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152435
更新日期:1991-01-01 00:00:00
abstract::This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009152156
更新日期:1990-01-01 00:00:00
abstract::Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TI...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160222
更新日期:1997-01-01 00:00:00
abstract::Raman spectra have been determined for hyaluronan, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan monomers and aggregates. The nature of the saccharides and the pattern of sulfation can be discerned. There were only small spectral changes with pH and ionic composition. Differences between hydroxyl vibrations, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802398422
更新日期:2009-01-01 00:00:00
abstract::Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum fr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009812
更新日期:1990-01-01 00:00:00
abstract::Proteoglycans extracted from articular cartilage of large joints of humans aged 4, 11, 70 and 75, were fractionated on associative density gradients. The top fraction (A3) was purified by ion-exchange chromatography and subsequent gel filtration on Sepharose CL 4B in 4 M GuCl, 0.5% Triton x 100. Proteoglycans from you...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809017475
更新日期:1988-01-01 00:00:00
abstract::Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200390229912
更新日期:2003-01-01 00:00:00
abstract::Two populations of primary osteoblasts and the cloned murine osteoblast cell line MC3T3 were activated with osteotropic agents and the conditioned media tested for the presence of macrophage colony stimulating factor (M-CSF). Differences in ability of the three populations to secrete the cytokine as well as in the eff...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023884
更新日期:1989-01-01 00:00:00
abstract::Muscle development in the embryonic chick involves the interfacing of two different cell types: myogenic cells forming the contractile network and connective tissue cells which wrap this network into discrete morphologies. Previous reports from this laboratory detailed the characterization of large extracellular matri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809015023
更新日期:1988-01-01 00:00:00
abstract::Osteogenesis imperfecta is a congenital disease commonly characterized by brittle bones and caused by mutations in the genes encoding Type I collagen, the single most abundant protein produced by the body. The oim model has a natural collagen mutation, converting its heterotrimeric structure (two α1 and one α2 chains)...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923860
更新日期:2014-08-01 00:00:00
abstract:OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951440
更新日期:2014-10-01 00:00:00
abstract::Although fibronectin (FN) modified by advanced glycation end products (AGEs) has been shown to contribute to the development of diabetic vascular complications through its reduced adhesive activity to vascular cells, little is known about changes in the cell binding domain of AGE-modified FN. Here we examined the mech...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009005291
更新日期:2000-01-01 00:00:00
abstract::This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovarie...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200902855917
更新日期:2010-01-01 00:00:00