Cellular interactions regulate stem cell differentiation in tri-culture.

Abstract:

:Currently, the mechanism governing the regeneration of the soft tissue-to-bone interface, such as the transition between the anterior cruciate ligament (ACL) and bone, is not known. Focusing on the ACL-to-bone insertion, this study tests the novel hypothesis that interactions between cells from the ligament (fibroblasts) and bone (osteoblasts) initiate interface regeneration. Specifically, these heterotypic cell interactions direct the fibrochondrogenic differentiation of interface-relevant cell populations, defined here as ligament fibroblasts and bone marrow stromal cells (BMSC). The objective of this study is to examine the effects of heterotypic cellular interactions on BMSC or fibroblast growth and biosynthesis, as well as expression of fibrocartilage-relevant markers in tri-culture. The effects of cell-cell physical contact and paracrine interactions between fibroblasts and osteoblasts were also determined. It was found that, in tri-culture with fibroblasts and osteoblasts, BMSC exhibited greater fibrochondrogenic potential than ligament fibroblasts. The growth of BMSC decreased while proteoglycan production and TGF-β3 expression increased. Moreover, tri-culture regulated BMSC response via paracrine factors, and interestingly, fibroblast-osteoblast contact further promoted proteoglycan and TGF-β1 synthesis as well as induced SOX9 expression in BMSC. Collectively, the findings of this study suggest that fibroblast-osteoblast interactions play an important role in regulating the stem cell niche for fibrocartilage regeneration, and the mechanisms of these interactions are directed by paracrine factors and augmented with direct cell-cell contact.

journal_name

Connect Tissue Res

authors

Wang IE,Bogdanowicz DR,Mitroo S,Shan J,Kala S,Lu HH

doi

10.1080/03008207.2016.1230106

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

476-487

issue

6

eissn

0300-8207

issn

1607-8438

journal_volume

57

pub_type

杂志文章
  • Initial characterization of small proteoglycans synthesized by embryonic chick leg muscle-associated connective tissues.

    abstract::Muscle development in the embryonic chick involves the interfacing of two different cell types: myogenic cells forming the contractile network and connective tissue cells which wrap this network into discrete morphologies. Previous reports from this laboratory detailed the characterization of large extracellular matri...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809015023

    authors: Young HE,Carrino DA,Caplan AI

    更新日期:1988-01-01 00:00:00

  • Structural analysis of glycosaminoglycans and proteoglycans by means of Raman microspectrometry.

    abstract::Raman spectra have been determined for hyaluronan, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan monomers and aggregates. The nature of the saccharides and the pattern of sulfation can be discerned. There were only small spectral changes with pH and ionic composition. Differences between hydroxyl vibrations, ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200802398422

    authors: Ellis R,Green E,Winlove CP

    更新日期:2009-01-01 00:00:00

  • TGF beta-1 downregulates DMP-1 and DSPP in odontoblasts.

    abstract::Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290000565

    authors: Unterbrink A,O'Sullivan M,Chen S,MacDougall M

    更新日期:2002-01-01 00:00:00

  • The effects of aging on the eruptive behavior of the mandibular incisor in male rats.

    abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290000754

    authors: Moxham BJ

    更新日期:2002-01-01 00:00:00

  • Modulation of growth factor action by the extracellular matrix.

    abstract::Growth factors were historically defined as molecules produced by the body to regulate cell growth and proliferation. After the identification of their receptors and the intracellular signaling machinery they activate, it is now clear that they are involved in the regulation of multiple processes essential for develop...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.1080/03008200802148629

    authors: Oehrl W,Panayotou G

    更新日期:2008-01-01 00:00:00

  • Temperospatial expression of matrix metalloproteinases 1, 2, 3, and 9 during early tooth development.

    abstract::Odontogenesis involves a complex series of processes including epithelial-mesenchymal interactions, morphogenesis, differentiation, fibrillogenesis, and mineralization. Extracellular (ECM) remodeling plays a critical role in the rapid morphological changes that accompany these events. It is proposed that matrix metall...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290000538

    authors: Randall LE,Hall RC

    更新日期:2002-01-01 00:00:00

  • Progenitor cells from dental follicle are able to form cementum matrix in vivo.

    abstract::To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290001023

    authors: Handa K,Saito M,Tsunoda A,Yamauchi M,Hattori S,Sato S,Toyoda M,Teranaka T,Narayanan AS

    更新日期:2002-01-01 00:00:00

  • Matrix metalloproteinase expression in keratocystic odontogenic tumors and primary cells.

    abstract:UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2014.923875

    authors: Amm HM,Casimir MD,Clark DB,Sohn P,MacDougall M

    更新日期:2014-08-01 00:00:00

  • Characterization of a bovine synovial fluid lubricating factor. III. The interaction with hyaluronic acid.

    abstract::Although hyaluronate is not a boundary lubricant in cartilaginous and latex:glass bearings, a distinct interaction with purified synovial lubricating factor (PSLF) was demonstrated by three means: 1) enhancement of lubricating ability in an artificial test system; 2) viscometry; 3) electron microscopy. The interaction...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209209016818

    authors: Jay GD,Lane BP,Sokoloff L

    更新日期:1992-01-01 00:00:00

  • Positional signalling and patterning for amelogenesis in mouse molar tooth development.

    abstract::Oral mandibular epithelium determines the position and number of tooth organs. Cranial neural crest-derived ectomesenchyme provides inductive signals for (i) tooth shape, and (ii) sequential determination and expression of several different epithelial-derived enamel proteins. Inductive signalling follows a precise seq...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.3109/03008208909023878

    authors: Slavkin HC

    更新日期:1989-01-01 00:00:00

  • Age-related changes in small proteoglycans of low buoyant density of human articular cartilage.

    abstract::Proteoglycans extracted from articular cartilage of large joints of humans aged 4, 11, 70 and 75, were fractionated on associative density gradients. The top fraction (A3) was purified by ion-exchange chromatography and subsequent gel filtration on Sepharose CL 4B in 4 M GuCl, 0.5% Triton x 100. Proteoglycans from you...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809017475

    authors: Stanescu V,Chaminade F,Muriel MP

    更新日期:1988-01-01 00:00:00

  • Incomplete elongation of the chondroitin sulfate linkage region on aggrecan and response to interleukin-1β.

    abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2012.756871

    authors: Frankenberger C,Borgia JA,Edirisinghe PD,Oegema TR

    更新日期:2013-01-01 00:00:00

  • Electron imaging and diffraction study of individual crystals of bone, mineralized tendon and synthetic carbonate apatite.

    abstract::A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109029158

    authors: Moradian-Oldak J,Weiner S,Addadi L,Landis WJ,Traub W

    更新日期:1991-01-01 00:00:00

  • In situ hybridization of calbindin-D 28 k transcripts in undecalcified sections of the rat continuously erupting incisor.

    abstract::Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509013716

    authors: Hotton D,Davideau JL,Bernaudin JF,Berdal A

    更新日期:1995-01-01 00:00:00

  • Effects of age and diabetes mellitus on cyanogen bromide digestion of human dura mater collagen.

    abstract::To extend previous studies on the apparent acceleration of collagen aging in diabetes mellitus, dura collagen from human adults of different ages, with and without diabetes, was processed to yield soluble and insoluble fractions. Insoluble fractions were cleaved by cyanogen bromide. Release of peptides from insoluble ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208309004852

    authors: Kohn RR

    更新日期:1983-01-01 00:00:00

  • Evidence for increased protease activity secreted from cultured fibroblasts from patients with pseudoxanthoma elasticum.

    abstract::Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152287

    authors: Gordon SG,Overland M,Foley J

    更新日期:1978-01-01 00:00:00

  • Collagen and elastin synthesis in human stroma and breast carcinoma cell lines: modulation by the extracellular matrix.

    abstract::The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208609017468

    authors: Kao RT,Hall J,Stern R

    更新日期:1986-01-01 00:00:00

  • Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs.

    abstract::Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progen...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2018.1553960

    authors: Mouser VHM,Levato R,Mensinga A,Dhert WJA,Gawlitta D,Malda J

    更新日期:2020-03-01 00:00:00

  • The visualization of individual collagen molecules and aggregates in collagen solution: the effect of preparation and sampling techniques.

    abstract::The use of electron microscopic observations to characterize the state of aggregation of collagen molecules in solution has been examined from the point of view that the observed structures must be compatible with the physical chemical properties of the solution. The common procedure of addition of droplets of a colla...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152630

    authors: Schwartz D,Veis A

    更新日期:1978-01-01 00:00:00

  • Expression of transcription factor Sox9 in rat L6 myoblastic cells.

    abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200490514130

    authors: Matsushita T,Matsui N,Fujioka H,Kubo S,Kuroda R,Kurosaka M,Yoshiya S

    更新日期:2004-01-01 00:00:00

  • Roles of large aggregating proteoglycans in human intervertebral disc degeneration.

    abstract::Degeneration of the intervertebral discs, a natural progression of the aging process, is strongly implicated as a cause of low back pain. Aggrecan is the major structural proteoglycan in the extracellular matrix of the intervertebral disc. It is large, possessing numerous glycosaminoglycan chains and the ability to fo...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.1080/03008207.2018.1499731

    authors: Wei Q,Zhang X,Zhou C,Ren Q,Zhang Y

    更新日期:2019-05-01 00:00:00

  • Heterogeneity in glycosylation of dermatan sulfate proteoglycan core proteins isolated from human aorta.

    abstract::Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209009009811

    authors: Register TC,Wagner WD

    更新日期:1990-01-01 00:00:00

  • Matrix accumulation and retention in embryonic cartilage and in vitro chondrogenesis.

    abstract::Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209609028895

    authors: Maleski MP,Knudson CB

    更新日期:1996-01-01 00:00:00

  • Characterization of a porcine amelogenin preparation, EMDOGAIN, a biological treatment for periodontal disease.

    abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290000880

    authors: Maycock J,Wood SR,Brookes SJ,Shore RC,Robinson C,Kirkham J

    更新日期:2002-01-01 00:00:00

  • Identification of genes required for the spontaneous repair of partial-thickness cartilage defects in immature rats.

    abstract:PURPOSE:Our previous study showed that partial-thickness articular cartilage defects (PTCDs) created in immature rats spontaneously healed to resemble normal hyaline cartilage, but that of mature rats did not. To identify molecules involved in the spontaneous cartilage repair observed in this model, gene expression was...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2015.1121250

    authors: Ikegawa N,Sasho T,Yamaguchi S,Saito M,Akagi R,Muramatsu Y,Akatsu Y,Fukawa T,Nakagawa K,Nakajima A,Suzuki T,Takahashi K

    更新日期:2016-05-01 00:00:00

  • Engineering 3D-Bioplotted scaffolds to induce aligned extracellular matrix deposition for musculoskeletal soft tissue replacement.

    abstract:PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2016.1276177

    authors: Warren PB,Huebner P,Spang JT,Shirwaiker RA,Fisher MB

    更新日期:2017-01-01 00:00:00

  • Abnormal dermal proteoglycan in aspartylglycosaminuria: a possible mechanism for ultrastructural changes of collagen fibrils in a glycoprotein storage disorder.

    abstract::Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase A...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208709005621

    authors: Näntö-Salonen K,Larjava H,Säämanen AM,Heino J,Penttinen R,Pelliniemi LJ,Tammi M

    更新日期:1987-01-01 00:00:00

  • Influence of substrate nature and immobilization of implanted dentin matrix components during induction of reparative dentinogenesis.

    abstract::The biological effects of isolated soluble dentin extracellular matrix components on the induction of reparative dentinogenesis in exposed cavities in ferret canine teeth have been shown to be blocked by immobilizing the extracellular matrix components on nitrocellulose or Millipore membranes during implantation. This...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509013736

    authors: Smith AJ,Tobias RS,Cassidy N,Bégue-Kirn C,Ruch JV,Lesot H

    更新日期:1995-01-01 00:00:00

  • Biochemical characterization of collagen in alveolar mucosa and attached gingiva of pig.

    abstract::Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collage...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200601143508

    authors: Katafuchi M,Matsuura T,Atsawasuwan P,Sato H,Yamauchi M

    更新日期:2007-01-01 00:00:00

  • Modulation of mesenchymal stem cell genotype and phenotype by extracellular matrix proteins.

    abstract:AIM:To investigate the effect of extracellular matrix (ECM) proteins on characteristics of mesenchymal stem cells (MSCs) and tendon-derived cells (TDCs). MATERIALS AND METHODS:MSCs and TDCs, cultured in a monolayer (2D) or hydrogels (3D), with or without ECM protein supplementation, and on a non-viable native tendon (...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2016.1215442

    authors: Clements LE,Garvican ER,Dudhia J,Smith RK

    更新日期:2016-11-01 00:00:00