Histochemical properties of the "crystal ghosts" of calcifying epiphyseal cartilage.

Abstract:

:The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These results suggest that crystal ghosts are, or derive from, proteoglycans of calcifying matrix.

journal_name

Connect Tissue Res

authors

Bonucci E,Silvestrini G,di Grezia R

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

43-50; discussion 53-61

issue

1-4

eissn

0300-8207

issn

1607-8438

journal_volume

22

pub_type

杂志文章
  • Generation of Hall voltage in bone.

    abstract::Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152629

    authors: Behari J,Andrabi WH

    更新日期:1978-01-01 00:00:00

  • Biochemical evaluation of organ cultures from primate flexor tendons.

    abstract::Recently our laboratory has reported, in a lacerated flexor tendon model, that the "early turnover" phase of the repair process extends for a longer period of time in vivo than previously documented. The extensive turnover of the collagenous matrix was consistent with the presence of collagenolytic activity in repairi...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208909103903

    authors: Russell JE,Manske PR

    更新日期:1989-01-01 00:00:00

  • Tendon cells derived from the long head of the biceps and the supraspinatus tendons of patients affected by rotator cuff tears show different expressions of inflammatory markers.

    abstract:AIM OF THE STUDY:Tendons are exposed to mechanical stress constantly during movements and thus they are frequently subjected to injuries. Rotator cuff tears are common musculoskeletal disorders, mainly involving the supraspinatus tendon. The characterization of the tenocytes derived from this tendon and the comparison ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2020.1816993

    authors: Viganò M,Lugano G,Orfei CP,Menon A,Ragni E,Colombini A,de Luca P,Talò G,Randelli PS,de Girolamo L

    更新日期:2020-09-14 00:00:00

  • The effects of aging upon the connective tissues of the periodontal ligament.

    abstract::The ultrastructural features of the cells and of the oxytalan fibers within the periodontal ligaments of aged rats (2 year old) were quantified and compared with data for tissues obtained from younger animals (8 week old). Sections of the mid-root regions of the mandibular first molars were prepared for examination by...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509016978

    authors: Moxham BJ,Evans IL

    更新日期:1995-01-01 00:00:00

  • Synergistic effect of combined growth factors in porcine intervertebral disc degeneration.

    abstract::Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2013.775258

    authors: Cho H,Lee S,Park SH,Huang J,Hasty KA,Kim SJ

    更新日期:2013-01-01 00:00:00

  • Arthrofibrosis and large joint scarring.

    abstract::Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are bei...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.1080/03008207.2018.1517759

    authors: Chen AF,Lee YS,Seidl AJ,Abboud JA

    更新日期:2019-01-01 00:00:00

  • Comparison of non-collagenous type IV collagen subunits in human glomerular basement membrane, alveolar basement membrane, and placenta.

    abstract::This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209009152156

    authors: Shen GQ,Butkowski R,Cheng T,Wieslander J,Katz A,Cass J,Fish AJ

    更新日期:1990-01-01 00:00:00

  • Collagenolytic enzymes assayed by spectrophotometry with suspensions of reconstituted collagen fibrils.

    abstract::Collagenolytic enzymes were quantitated by a method based on spectrophotometry of suspended reconstituted collagen fibrils. To obtain optically stable suspensions it was necessary to perform a short sonication of the aggregated fibrils at 10 degrees C. When fibrils were cleaved with mammalian fibroblast collagenase at...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109152442

    authors: Bleeg HS

    更新日期:1991-01-01 00:00:00

  • Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs.

    abstract::Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progen...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2018.1553960

    authors: Mouser VHM,Levato R,Mensinga A,Dhert WJA,Gawlitta D,Malda J

    更新日期:2020-03-01 00:00:00

  • The visualization of individual collagen molecules and aggregates in collagen solution: the effect of preparation and sampling techniques.

    abstract::The use of electron microscopic observations to characterize the state of aggregation of collagen molecules in solution has been examined from the point of view that the observed structures must be compatible with the physical chemical properties of the solution. The common procedure of addition of droplets of a colla...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152630

    authors: Schwartz D,Veis A

    更新日期:1978-01-01 00:00:00

  • Proteoglycan-4 and hyaluronan composition in synovial fluid and serum from clinical equine subjects: relationship to cartilage boundary lubrication and viscosity of synovial fluid.

    abstract::Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2020.1751140

    authors: Matheson A,Regmi SC,Martin-Alarcon L,Jay GD,Scott WM,Schmidt TA

    更新日期:2020-04-20 00:00:00

  • The Fras1/Frem family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype.

    abstract::Fras1 and the structurally related proteins Frem1, Frem2, and Frem3, comprise a novel family of extracellular matrix proteins, which localize in a similar fashion underneath the lamina densa of epithelial basement membranes. They are involved in the structural adhesion of the skin epithelium to its underlying mesenchy...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200802148025

    authors: Petrou P,Makrygiannis AK,Chalepakis G

    更新日期:2008-01-01 00:00:00

  • Collagen and elastin synthesis in human stroma and breast carcinoma cell lines: modulation by the extracellular matrix.

    abstract::The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208609017468

    authors: Kao RT,Hall J,Stern R

    更新日期:1986-01-01 00:00:00

  • Calcite orientations and composition ranges within teeth across Echinoidea.

    abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2014.923865

    authors: Stock SR,Ignatiev K,Lee PL,Almer JD

    更新日期:2014-08-01 00:00:00

  • Two distinctive BMP-carriers induce zonal chondrogenesis and membranous ossification, respectively; geometrical factors of matrices for cell-differentiation.

    abstract::A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509013726

    authors: Kuboki Y,Saito T,Murata M,Takita H,Mizuno M,Inoue M,Nagai N,Poole AR

    更新日期:1995-01-01 00:00:00

  • An address to young research workers: Inconsequences and blindness to the facts and unpublished observations.

    abstract::The critical remembrance of my research activity displays aspects of inconsequence and mental rigidity. Particular probative examples are called to mind. Unpublished observations concerning immortalized dental cells are summarized. As a consequence, if I could start again, I would improve several aspects of my behavio...

    journal_title:Connective tissue research

    pub_type:

    doi:10.1080/03008200290001195

    authors: Ruch JV

    更新日期:2002-01-01 00:00:00

  • In situ hybridization of calbindin-D 28 k transcripts in undecalcified sections of the rat continuously erupting incisor.

    abstract::Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509013716

    authors: Hotton D,Davideau JL,Bernaudin JF,Berdal A

    更新日期:1995-01-01 00:00:00

  • Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis.

    abstract::Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endo...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200903056150

    authors: Antoniou KM,Soufla G,Lymbouridou R,Economidou F,Lasithiotaki I,Manousakis M,Drositis I,Spandidos DA,Siafakas NM

    更新日期:2010-01-01 00:00:00

  • Proinflammatory cytokines regulate the gene expression of hyaluronic acid synthetase in cultured rabbit synovial membrane cells.

    abstract::To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200109005649

    authors: Tanimoto K,Ohno S,Fujimoto K,Honda K,Ijuin C,Tanaka N,Doi T,Nakahara M,Tanne K

    更新日期:2001-01-01 00:00:00

  • Initial characterization of small proteoglycans synthesized by embryonic chick leg muscle-associated connective tissues.

    abstract::Muscle development in the embryonic chick involves the interfacing of two different cell types: myogenic cells forming the contractile network and connective tissue cells which wrap this network into discrete morphologies. Previous reports from this laboratory detailed the characterization of large extracellular matri...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809015023

    authors: Young HE,Carrino DA,Caplan AI

    更新日期:1988-01-01 00:00:00

  • Effect of thyroid hormone deficiency on proteoglycan synthesis by human skin fibroblast cultures.

    abstract::Proteoglycans and hyaluronic acid synthesized by human skin fibroblasts in culture were characterized, and the effect of thyroid hormone deficiency was examined. The fibroblasts in culture synthesize hyaluronic acid, dermatan sulfate (DS) proteoglycans and heparan sulfate (HS) proteoglycans. Hyaluronic acid is almost ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809015024

    authors: Shishiba Y,Yanagishita M,Hascall VC

    更新日期:1988-01-01 00:00:00

  • Characterization of the bone sialoprotein (BSP) gene promoter.

    abstract::Bone sialoprotein is a 34 kDa phosphorylated and sulphated glycoprotein that is essentially unique to mineralizing connective tissues. Recent studies on the developmental expression of BSP mRNA and the temporo-spatial appearance of the protein during bone formation in vivo and in vitro have demonstrated that BSP is ex...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209609029171

    authors: Sodek J,Li JJ,Kim RH,Ogata Y,Yamauchi M

    更新日期:1996-01-01 00:00:00

  • Substance P enhanced titanium particles-induced RANKL expression in fibroblasts from periprosthetic membrane.

    abstract::Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to the failure. Substance P (SP) immunoreactive nerve fibers have been detected in the periprosthetic membrane of aseptic loose hip prostheses. We isolat...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2013.823953

    authors: Qian Y,Zhang XL,Zeng BF,Jiang Y,Shen H,Wang Q

    更新日期:2013-01-01 00:00:00

  • Orthotic management for children with osteogenesis imperfecta.

    abstract::Fitting children and infants who have osteogenesis imperfecta (OI) with braces has posed substantial problems of implementation and patient management For the past twelve years bracing has been an important component of a patient research and management program conducted at the National Institutes of Health. By using ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509116832

    authors: Weintrob JC

    更新日期:1995-01-01 00:00:00

  • A restriction fragment length polymorphism in the pigeon pro alpha 2(1) collagen gene: lack of an allelic association with an atherogenic phenotype in pigeons genetically susceptible to the development of spontaneous atherosclerosis.

    abstract::A high frequency restriction fragment length polymorphism (RFLP) at the 3'-end of the pigeon pro alpha 2(1) collagen gene was detected using the restriction endonuclease EcoR1. The distribution of this allelic variant was analyzed in DNA isolated from White Carneau pigeons genetically susceptible to the development of...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109152437

    authors: Boyd CD,Song JY,Kniep AC,Park HS,Fastnacht C,Smith EC,Smith SC

    更新日期:1991-01-01 00:00:00

  • Matrix accumulation and retention in embryonic cartilage and in vitro chondrogenesis.

    abstract::Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209609028895

    authors: Maleski MP,Knudson CB

    更新日期:1996-01-01 00:00:00

  • Dental structural diseases mapping to human chromosome 4q21.

    abstract::Genetic diseases affecting tooth structure have been classified by the tissue affected enamel versus dentin, and their pattern of inheritance autosomal dominant, autosomal recessive, or X-linked. Advances in molecular genetics and the Human Genome Project have provided substantial progress regarding the identification...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:

    authors: MacDougall M

    更新日期:2003-01-01 00:00:00

  • Insulin and thyroid hormones stimulate matrix metabolism in primary cultures of articular chondrocytes from young rabbits independently and in combination.

    abstract::These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209409005633

    authors: Glade MJ,Kanwar YS,Stern PH

    更新日期:1994-01-01 00:00:00

  • A comparison of bovine nasal cartilage proteoglycan core protein produced by chondroitinase and hyaluronidase: the possible role of protease contaminants.

    abstract::The basic subunit of cartilage proteoglycan consists of multiple glycosaminoglycan chains covalently attached to a core protein. It is unclear as to whether there is a single core protein or multiple different core proteins, since previous studies using either chondroitinase or testicular hyaluronidase to enzymaticall...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207709152265

    authors: Keiser HD,Hatcher VB

    更新日期:1977-01-01 00:00:00

  • Pathology of experimental disuse muscular atrophy in rats.

    abstract::The relationship between structural changes and hyaluronic acid (HA) content in muscular atrophy is unknown. The objective of our study was to conduct multifaceted evaluations of the change in muscle extensibility in muscular atrophy, in terms of morphological aspects as well as from a biochemical viewpoint, including...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200590954131

    authors: Ishikawa T,Shimizu M,Mikawa Y,Zhu BL,Quan L,Li DR,Zhao D,Maeda H

    更新日期:2005-01-01 00:00:00