Insulin and thyroid hormones stimulate matrix metabolism in primary cultures of articular chondrocytes from young rabbits independently and in combination.

abstract：:Recently our laboratory has reported, in a lacerated flexor tendon model, that the "early turnover" phase of the repair process extends for a longer period of time in vivo than previously documented. The extensive turnover of the collagenous matrix was consistent with the presence of collagenolytic activity in repairi...

journal_title：Connective tissue research

authors： Russell JE,Manske PR

更新日期：1989-01-01 00:00:00

abstract：:Although bone is a nanocomposite of mineral and collagen, mineral has been the more elusive component of study. A standard for bone mineral is clearly needed. We hypothesized that the most natural, least-processed bone mineral could be retrieved from the most highly mineralized bone. We therefore studied the rostrum o...

journal_title：Connective tissue research

authors： Li Z,Pasteris JD,Novack D

更新日期：2013-01-01 00:00:00

abstract：:Positive effects on the tensile characteristics of swine digital extensors were found following twelve months of exercise training. Compared to sedentary controls, the tendons from the exercised animals became stronger as a material and exhibited hypertrophy. These biomechanical results were supported by biochemical a...

journal_title：Connective tissue research

authors： Woo SL,Ritter MA,Amiel D,Sanders TM,Gomez MA,Kuei SC,Garfin SR,Akeson WH

更新日期：1980-01-01 00:00:00

abstract：AIMS:The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate th...

journal_title：Connective tissue research

authors： Bertassoni LE,Swain MV

更新日期：2017-09-01 00:00:00

abstract：:The present study details structural aspects of the cells of the rat intra-articular disc (IAD) of the temporomandibular joint. At the light and electron microscope level, the outline of the cells varied, although a number appeared oval/rounded. Ultrastructurally, the cells contained moderate amounts of the intracellu...

journal_title：Connective tissue research

authors： Berkovitz BK,Becker D

更新日期：2003-01-01 00:00:00

abstract：:We determined the feasibility of using scaffolds of adenoviral human BMP2 gene (AdBMP2)-modified human bone marrow mesenchymal stem cells (hBMSCs) and antigen-free bovine cancellous bone (BCB) to construct bone tissue. hMSCs were infected with AdBMP-2. Expression of BMP-2 and alkaline phosphatase confirmed successful ...

journal_title：Connective tissue research

authors： Han D,Li J,Guan X

更新日期：2010-08-01 00:00:00

abstract：:Osteoarthritis (OA), the most common form of degenerative joint disease, is linked to high morbidity. It is predicted to be the single greatest cause of disability in the general population by 2030. The development of disease-modifying therapy for OA currently face great obstacle mainly because the onset and developme...

journal_title：Connective tissue research

authors： Wei Y,Bai L

更新日期：2016-07-01 00:00:00

abstract：:Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...

journal_title：Connective tissue research

authors： Hosaka Y,Kirisawa R,Mafune N,Takehana K

更新日期：2005-01-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement ...

journal_title：Connective tissue research

authors： Kemeny E,Fillit HM,Damle S,Mahabir R,Kefalides NA,Gregory JD,Antonovych T,Sabnis S,Zabriskie JB

更新日期：1988-01-01 00:00:00

abstract：:Cumulative damage to long-lived connective tissue proteins play a key role in the development of age-related human diseases such as cardiovascular stiffening and age-related macular degeneration. The processes that result in the accumulation of increasingly insoluble, undigestible damaged collagen are only partially k...

journal_title：Connective tissue research

authors： Paik DC,Saito LY,Sugirtharaj DD,Holmes JW

更新日期：2006-01-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenovirus...

journal_title：Connective tissue research

authors： Jin QM,Zhao M,Economides AN,Somerman MJ,Giannobile WV

更新日期：2004-01-01 00:00:00

abstract：:Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...

journal_title：Connective tissue research

authors： Giraud-Guille MM,Besseau L

更新日期：1998-01-01 00:00:00

abstract：:The novel method of high voltage electron microscopic tomography (3D) has been applied for the first time to examine ultrastructural features and spatial relations between collagen fibrils and mineral crystals in a mouse mutant (oim/oim) which replicates a moderate to severe form of osteogenesis imperfecta. The animal...

journal_title：Connective tissue research

authors： Landis WJ

更新日期：1995-01-01 00:00:00

abstract：:Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...

journal_title：Connective tissue research

authors： Gopalakrishnan R,Thomas PE,Benson MD,Wang D,Franceschi RT

更新日期：2003-01-01 00:00:00

abstract：:A high frequency restriction fragment length polymorphism (RFLP) at the 3'-end of the pigeon pro alpha 2(1) collagen gene was detected using the restriction endonuclease EcoR1. The distribution of this allelic variant was analyzed in DNA isolated from White Carneau pigeons genetically susceptible to the development of...

journal_title：Connective tissue research

authors： Boyd CD,Song JY,Kniep AC,Park HS,Fastnacht C,Smith EC,Smith SC

更新日期：1991-01-01 00:00:00

abstract：PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...

journal_title：Connective tissue research

authors： Kanaya S,Komatsu H,Shimauchi H,Nemoto E

更新日期：2016-09-01 00:00:00

abstract：:Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...

journal_title：Connective tissue research

authors： Landis WJ,Jacquet R,Hillyer J,Zhang J

更新日期：2003-01-01 00:00:00

abstract：:Although hyaluronate is not a boundary lubricant in cartilaginous and latex:glass bearings, a distinct interaction with purified synovial lubricating factor (PSLF) was demonstrated by three means: 1) enhancement of lubricating ability in an artificial test system; 2) viscometry; 3) electron microscopy. The interaction...

journal_title：Connective tissue research

authors： Jay GD,Lane BP,Sokoloff L

更新日期：1992-01-01 00:00:00

abstract：:To study the glycosylation of glomerular basement membrane collagen (GBMC) in diabetes, kidneys were obtained at autopsy from 5 patients with insulin-requiring diabetes of long duration and diabetic complications, and from 5 control subjects. Glomeruli were prepared by sieving and collagen was isolated by limited peps...

journal_title：Connective tissue research

authors： Uitto J,Perejda AJ,Grant GA,Rowold EA,Kilo C,Williamson JR

更新日期：1982-01-01 00:00:00

abstract：:The diameter of collagen fibrils has been measured in electron micrographs of the tunica media and adventitia of the common carotid artery of 14 rats (19,262 counts). The fibrils of the media were measured in each of its three interlamellar spaces. The mean fibril diameters in the innermost space and the middle space ...

journal_title：Connective tissue research

authors： Buck RC

更新日期：1987-01-01 00:00:00

abstract：:This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...

journal_title：Connective tissue research

authors： Shen GQ,Butkowski R,Cheng T,Wieslander J,Katz A,Cass J,Fish AJ

更新日期：1990-01-01 00:00:00

abstract：:The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...

journal_title：Connective tissue research

authors： Keiser HD,Malemud CJ

更新日期：1983-01-01 00:00:00

abstract：:The experiments described herein tested the effects of CaCl2 and ZnCl2, added at various concentrations in the culture medium, upon the synthesis of collagen and proteoglycan by adult and fetal (articular, epiphyseal and hypertrophic) bovine chondrocytes maintained in high density multilayer cultures. CaCl2 concentrat...

journal_title：Connective tissue research

authors： Koyano Y,Hejna M,Flechtenmacher J,Schmid TM,Thonar EJ,Mollenhauer J

更新日期：1996-01-01 00:00:00

abstract：:The small leucine-rich repeat proteoglycans (SLRPs) constitute a group of structurally and functionally related molecules that participate in the organization of the extracellular matrix ECM and have important effects on cell behavior. Osteosarcomas are heterogeneous bone tumors whose common characteristic is the prod...

journal_title：Connective tissue research

authors： Nikitovic D,Berdiaki K,Chalkiadaki G,Karamanos N,Tzanakakis G

更新日期：2008-01-01 00:00:00

abstract：:Collagen type I fiber-based scaffolds for anterior cruciate ligament (ACL) replacement were evaluated for their mechanical properties and their ability to promote cellular proliferation. Prior to scaffold formation, two crosslinking methods were investigated on individual reconstituted collagen type I fibers, ultravio...

journal_title：Connective tissue research

authors： Walters VI,Kwansa AL,Freeman JW

更新日期：2012-01-01 00:00:00

abstract：:Short term cultures were carried out with chondrocytes and tissue fragments from fetal guinea pig epiphyseal cartilage. Proteoglycans were isolated from these cultures and their properties were compared with those of proteoglycans from adult hyaline cartilage. It was concluded that the proteoglycans synthesized in cul...

journal_title：Connective tissue research

authors： Madsen K,Lohmander S,Moskalewski S,Hinek A

更新日期：1978-01-01 00:00:00

abstract：:We previously reported that circulating peripheral blood-borne cells (PBCs) contribute to early-phase meniscal reparative change. Because macrophages and myofibroblasts are important contributors of tissue regeneration, we examined their origin and distribution in the reparative meniscus. Reparative menisci were evalu...

journal_title：Connective tissue research

authors： Terai S,Hashimoto Y,Orita K,Yamasaki S,Takigami J,Shinkuma T,Teraoka T,Nishida Y,Takahashi M,Nakamura H

更新日期：2017-11-01 00:00:00

abstract：:The efficacy of connective tissue explants is difficult to determine, particularly where autopsy material is required for research or clinical applications. We report here an optimised protocol using 5-chloromethylfluorescein diacetate (CMFDA) and ethidium homodimer-1 to distinguish viable and non-viable cells in a ra...

journal_title：Connective tissue research

authors： Poole CA,Brookes NH,Gilbert RT,Beaumont BW,Crowther A,Scott L,Merrilees MJ

更新日期：1996-01-01 00:00:00

abstract：:Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared...

journal_title：Connective tissue research

authors： Kemppainen J,Yu Q,Alexander J,Jacquet R,Scharschmidt T,Landis W

更新日期：2014-08-01 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endo...

journal_title：Connective tissue research

authors： Antoniou KM,Soufla G,Lymbouridou R,Economidou F,Lasithiotaki I,Manousakis M,Drositis I,Spandidos DA,Siafakas NM

更新日期：2010-01-01 00:00:00