Abstract:
:The experiments described herein tested the effects of CaCl2 and ZnCl2, added at various concentrations in the culture medium, upon the synthesis of collagen and proteoglycan by adult and fetal (articular, epiphyseal and hypertrophic) bovine chondrocytes maintained in high density multilayer cultures. CaCl2 concentrations below 0.5 mM or the addition of 1-50 microM ZnCl2 to the medium selectively promoted the production of collagen by all four populations of chondrocytes but had no effect on fibroblasts. Further, these changes had no statistically significant effect on the incorporation of 35S-sulfate into macromolecules or on the synthesis of gelatinase A, measured by gelatin zymography. The addition of CaCl2 and ZnCl2 at these concentrations did not result in a change in the relative proportion of non-crosslinked 3H-collagen molecules (synthesized in the presence of beta-aminopropionitrile) partitioning in the cell layer and medium compartments, and did not appreciably alter the pattern of collagens synthesized by any of the cell populations. The hypertrophic cells synthesized high levels of collagen type X in the presence as well as absence of exogenously added cations. However, CaCl2 at 10 mM caused a marked upregulation of collagen type X synthesis by a preparation of chondrocytes derived from the entire growth plate, consistent with the view that calcium at that concentration stimulated the differentiation of some of the cells into hypertrophic chondrocytes.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Koyano Y,Hejna M,Flechtenmacher J,Schmid TM,Thonar EJ,Mollenhauer Jdoi
10.3109/03008209609000700subject
Has Abstractpub_date
1996-01-01 00:00:00pages
213-25issue
3eissn
0300-8207issn
1607-8438journal_volume
34pub_type
杂志文章abstract::Collagenolytic enzymes were quantitated by a method based on spectrophotometry of suspended reconstituted collagen fibrils. To obtain optically stable suspensions it was necessary to perform a short sonication of the aggregated fibrils at 10 degrees C. When fibrils were cleaved with mammalian fibroblast collagenase at...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152442
更新日期:1991-01-01 00:00:00
abstract::STR/ort mice develop a naturally occurring osteoarthritis (OA) of the knee joints. However, the evaluation of early OA changes has been difficult due to variability caused by gender, individual differences, and differences between the right and left lower limbs. The objective of this study was to analyze the variabili...
journal_title:Connective tissue research
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abstract::To study the glycosylation of glomerular basement membrane collagen (GBMC) in diabetes, kidneys were obtained at autopsy from 5 patients with insulin-requiring diabetes of long duration and diabetic complications, and from 5 control subjects. Glomeruli were prepared by sieving and collagen was isolated by limited peps...
journal_title:Connective tissue research
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doi:10.3109/03008208209008054
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abstract::To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...
journal_title:Connective tissue research
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更新日期:1999-01-01 00:00:00
abstract::Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase A...
journal_title:Connective tissue research
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abstract::Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due t...
journal_title:Connective tissue research
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abstract::A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...
journal_title:Connective tissue research
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更新日期:1991-01-01 00:00:00
abstract::In the absence of ascorbic acid, confluent human skin fibroblasts incubated in 0.5% serum-supplemented medium had one-third of the level of lysyl hydroxylase activity of cells incubated in media containing high serum concentrations (5-20%). This difference appeared to be due to a decline in the enzyme activity followi...
journal_title:Connective tissue research
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更新日期:1985-01-01 00:00:00
abstract::Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are bei...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
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abstract::The thermal stability and solubility profiles of human hypertrophic scar collagen have been measured and compared with those of the collagens present in human normal scar, human normal skin and a lathyritic dermal tissue. The thermal stability profiles of the hypertrophic scar collagens were found to be very similar t...
journal_title:Connective tissue research
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更新日期:1984-01-01 00:00:00
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journal_title:Connective tissue research
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更新日期:2011-01-01 00:00:00
abstract::Effects of applied hydrostatic pressure on transmembrane potentials were investigated in sheep articular chondrocytes and human skin fibroblasts in non-confluent monolayer cultures. Resting potentials in chondrocytes (about -12 mv) and in fibroblasts (about -15 mV) were increased and decreased respectively by over 40%...
journal_title:Connective tissue research
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doi:10.3109/03008209209014227
更新日期:1992-01-01 00:00:00
abstract::Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collage...
journal_title:Connective tissue research
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doi:10.1080/03008200601143508
更新日期:2007-01-01 00:00:00
abstract::We have investigated the changes in some of the biochemical and biophysical properties of human femoral head cartilage on the one hand during ageing and on the other hand in osteoarthritis. Topographical variations were also investigated. The parameters studied were those relevant to cartilage function, viz., proteogl...
journal_title:Connective tissue research
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doi:10.3109/03008208909043895
更新日期:1989-01-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...
journal_title:Connective tissue research
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doi:10.3109/03008207.2014.882331
更新日期:2014-06-01 00:00:00
abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...
journal_title:Connective tissue research
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更新日期:2000-01-01 00:00:00
abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
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更新日期:2016-09-01 00:00:00
abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...
journal_title:Connective tissue research
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更新日期:1988-01-01 00:00:00
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更新日期:2002-01-01 00:00:00
abstract::The in-vitro fluoride treatment technique has been introduced to investigate the composite behavior of bone tissue. Bone tissue with different mechanical properties can be obtained by varying the concentration, pH and immersion time in fluoride ion solutions. The chemical and physical changes in intact pieces of bone ...
journal_title:Connective tissue research
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doi:
更新日期:2002-01-01 00:00:00
abstract::This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups wer...
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journal_title:Connective tissue research
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更新日期:1987-01-01 00:00:00
abstract:PURPOSE:Orthodontic tooth movement occurs during the bone remodeling induced by therapeutic mechanical strain. It is important to investigate the relation between the strength of mechanical stress and bone formation activity. The aim of this study was to determine the effect of high-magnitude mechanical strain on bone ...
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更新日期:2015-01-01 00:00:00
abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...
journal_title:Connective tissue research
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更新日期:1998-01-01 00:00:00
abstract::Genetic diseases affecting tooth structure have been classified by the tissue affected enamel versus dentin, and their pattern of inheritance autosomal dominant, autosomal recessive, or X-linked. Advances in molecular genetics and the Human Genome Project have provided substantial progress regarding the identification...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
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更新日期:2003-01-01 00:00:00
abstract::Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to the failure. Substance P (SP) immunoreactive nerve fibers have been detected in the periprosthetic membrane of aseptic loose hip prostheses. We isolat...
journal_title:Connective tissue research
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更新日期:2013-01-01 00:00:00
abstract::Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...
journal_title:Connective tissue research
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更新日期:1990-01-01 00:00:00
abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...
journal_title:Connective tissue research
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更新日期:2020-12-11 00:00:00
abstract:BACKGROUND:The presence of genetic variants in uric acid (UA) transporters can be associated with hyperuricemia, and therefore with an increased risk of monosodium urate (MSU) crystal precipitation. The inflammatory process triggered by these crystals leads to cartilage damage, which, in turn, could promote knee osteoa...
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更新日期:2019-05-01 00:00:00
abstract::The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabo...
journal_title:Connective tissue research
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更新日期:2008-01-01 00:00:00