Abstract:
OBJECTIVE:Excessive apoptosis plays an important role in the progression of intervertebral disc degeneration. However, the effect of autophagy, another type of programmed cell death, on the pathogenesis of disc degeneration is still unclear. Macroautophagy and chaperone-mediated autophagy (CMA) change and intervertebral disc degeneration aggravates with age. This study aims at examining the expression changes of light chain 3 (LC3), lysosome-associated membrane protein 2A (LAMP-2A), and Hsc70, the indicator substrates of macroautophagy and CMA, in rat nucleus pulposus (NP) to prove that macroautophagy and CMA are both related with age. METHODS:Female Sprague-Dawley rats of 3, 12, and 24 months (n = 8 per age) were used in this study. Autophagic vacuoles in NP cells were detected by transmission electron microscopy. In NP, the expressions of LC3-II and LAMP-2A protein and mRNA were examined by immunohistochemistry and reverse transcription polymerase chain reaction, respectively. LC3-II, LC3-I, and LAMP-2A protein were also measured by western blot. The mRNA and protein level of myocyte enhancer factor-2D regulated by LAMP-2A and Hsc70 were detected by reverse transcriptase polymerase chain reaction and western blot, respectively. RESULTS:Transmission electron microscopy showed more autophagic vacuoles in 12- and 24-month groups than in 3-month group. Expression of LC3-II and LC3-II/LC3-I in 24-month group was significantly higher than in 3-month group (p < 0.05). Meanwhile, LAMP-2A expression was significantly higher in 24-month group than in 3-month group (p < 0.05). However, lower expression of Hsc70 and myocyte enhancer factor-2D was found in the 24-month rats than in 3-month group (p < 0.05, p < 0.05, respectively). CONCLUSION:Macroautophagy and CMA were present and increased with age in rat NP.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Ye W,Xu K,Huang D,Liang A,Peng Y,Zhu W,Li Cdoi
10.3109/03008207.2011.564336subject
Has Abstractpub_date
2011-01-01 00:00:00pages
472-8issue
6eissn
0300-8207issn
1607-8438journal_volume
52pub_type
杂志文章abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152166
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abstract::The novel method of high voltage electron microscopic tomography (3D) has been applied for the first time to examine ultrastructural features and spatial relations between collagen fibrils and mineral crystals in a mouse mutant (oim/oim) which replicates a moderate to severe form of osteogenesis imperfecta. The animal...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010825
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abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title:Connective tissue research
pub_type: 杂志文章
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abstract::Transforming growth factor beta (TGF-beta) is a ubiquitous regulator of cellular growth and differentiation. The present study investigated the effects of TGF-beta on chick growth plate chondrocyte proliferation, matrix synthesis, and alkaline phosphatase activity in short term cultures. TGF-beta markedly stimulated D...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023900
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abstract::To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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abstract::Fras1 and the structurally related proteins Frem1, Frem2, and Frem3, comprise a novel family of extracellular matrix proteins, which localize in a similar fashion underneath the lamina densa of epithelial basement membranes. They are involved in the structural adhesion of the skin epithelium to its underlying mesenchy...
journal_title:Connective tissue research
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160210
更新日期:1997-01-01 00:00:00
abstract::A detailed analysis of the D-period and axial electron density distribution of cornea and tendon collagen was carried out by means of X-ray diffraction and electron microscopy. Ultrastructural observations were made on replicas of freeze fractured and deep-etched specimens. Synchrotron radiation was used to obtain hig...
journal_title:Connective tissue research
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Connective tissue research
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doi:10.3109/03008208609017468
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1276177
更新日期:2017-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109006995
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abstract::Depolymerization of Ch-4-S, Ch-6-S, HA and HP was observed by gel filtration after treatment with 2-10 N NaOH or Ba(OH)2 in the presence of NaBH4 at 80-83 degrees for 1-5 hr. Depolymerized products were isolated after these treatments. 4-Deoxy-alpha-L-threo-4-enohexopyranosyluronic acid residue as the nonreducing end ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207509152343
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journal_title:Connective tissue research
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doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract::Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...
journal_title:Connective tissue research
pub_type: 杂志文章
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journal_title:Connective tissue research
pub_type: 杂志文章,评审
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abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
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abstract:AIM OF THE STUDY:To further elucidate the pathogenesis of systemic sclerosis (SSc) an experimental avian model was used. The University of California at Davis line 200 (UCD-200) chickens spontaneously develop a SSc-like disease that has most features of human SSc with vascular effects, inflammation, autoimmunity, and f...
journal_title:Connective tissue research
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pub_type: 临床试验,杂志文章
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journal_title:Connective tissue research
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abstract::Purpose: The osteocyte is considered the major mechanosensor in bone, capable of detecting forces at a cellular level to coordinate bone formation and resorption. The pathology of age-related bone loss, a hallmark of osteoporosis, is attributed in part to impaired osteocyte mechanosensing. However, real-time evidence ...
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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doi:10.3109/03008208309005609
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abstract::By using ultramorphological and biochemical methods, we analyzed the regional differences between the three parts of the equine superficial digital flexor tendon (SDFT), namely, the myotendinous junction (MTJ), middle metacarpal (mM), and osteotendinous junction (OTJ). Cross-sectional images showed unique distribution...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title:Connective tissue research
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更新日期:1998-01-01 00:00:00