Abstract:
AIMS:The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate the nanostructural organization of demineralized dentin collagen following the digestion of non-collagenous components with a trypsin enzyme. MATERIALS AND METHODS:Dentin specimens were obtained from healthy third-molars, cut into small cubes, and polished down to 1 µm roughness. Samples were then demineralized with 10% citric acid for 2 min. Selected specimens were further treated with a solution containing 1 mg/ml trypsin for 48 hours at 37 °C (pH 7.9-9.0). Both untreated and trypsin digested samples were analyzed using SDS-PAGE, Field Emission Scanning Electron Microscopy (FE-SEM), and nanoindentation, where surface hardness and creep properties were compared before and after treatments. RESULTS:FE-SEM images of demineralized dentin showed the banded morphology of D-periodical collagen type I, which upon enzymatic digestion with trypsin appeared to dissociate longitudinally, consistently unraveling ~20 nm structures (microfibril bundles). Such nanoscale structures, to the best of our knowledge, have not been characterized in dentin previously. Mechanical characterization via nanoindentation showed that the unraveling of such microfibril bundles affected the creep displacement and creep rate of demineralized dentin. CONCLUSION:In summary, our results provide novel evidence of the organization of collagen type I from dentin, which may have important implications for the interaction of dental materials with the organic dentin matrix and the mechanical properties of mineralized tissues.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Bertassoni LE,Swain MVdoi
10.1080/03008207.2016.1235566subject
Has Abstractpub_date
2017-09-01 00:00:00pages
414-423issue
5eissn
0300-8207issn
1607-8438journal_volume
58pub_type
杂志文章abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409013694
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abstract::Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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更新日期:2002-01-01 00:00:00
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journal_title:Connective tissue research
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doi:
更新日期:2009-01-01 00:00:00
abstract::Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular pro...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
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更新日期:1990-01-01 00:00:00
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更新日期:2008-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
abstract::The pharmacokinetics of desmosine following a 5 mg intravenous dose was determined in five sheep. The biological half-life (t 1/2 beta) of desmosine varied between 81-489 min. Urinary recovery of unchanged desmosine indicated that the compound is not extensively metabolized and that the primary route of elimination is...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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更新日期:1985-01-01 00:00:00