Abstract:
:Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,000 (185K), 175,000 (175K) and 150,000 (150K). The 185K and 175K components appeared to be similar to the pro-alpha 1 (IV) and pro-alpha 2(IV) chains, respectively, which have been isolated from other sources. These components were completely susceptible to bacterial collagenase, but were only partially susceptible to alpha-chymotrypsin digestion. The 350K and 220K components appeared to be similar to subunits of laminin (or PYS A and PYS B, respectively) which have been characterized by others, while the 150K component may be similar to entactin (or PYS C). These components were completely resistant to bacterial collagenase and completely susceptible to alpha-chymotrypsin digestion. In addition, the basement membrane of the parietal yolk sac (Reichert's membrane) stained intensely with antibodies directed against either rat laminin or mouse basement membrane procollagen. The results of these experiments suggest that the 14.5-day rat embryo parietal yolk sac is a useful system for studying the structure, biosynthesis and deposition of basement membrane components.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Clark CC,Kefalides NAdoi
10.3109/03008208209008056subject
Has Abstractpub_date
1982-01-01 00:00:00pages
303-18issue
3-4eissn
0300-8207issn
1607-8438journal_volume
10pub_type
杂志文章abstract::We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309015011
更新日期:1983-01-01 00:00:00
abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923865
更新日期:2014-08-01 00:00:00
abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029217
更新日期:1996-01-01 00:00:00
abstract:UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923875
更新日期:2014-08-01 00:00:00
abstract::Volume and morphology of chondrocytes in osteoarthritic human hip joint articular cartilage were characterized, and their relationship to tissue structure and function was determined. Human osteochondral articular cartilage samples (n=16) were obtained from the femoral heads of nine patients undergoing total hip arthr...
journal_title:Connective tissue research
pub_type: 临床试验,杂志文章
doi:10.3109/03008207.2014.912645
更新日期:2014-08-01 00:00:00
abstract::Positive effects on the tensile characteristics of swine digital extensors were found following twelve months of exercise training. Compared to sedentary controls, the tendons from the exercised animals became stronger as a material and exhibited hypertrophy. These biomechanical results were supported by biochemical a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208009152109
更新日期:1980-01-01 00:00:00
abstract::Purpose/Aim: Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from vascular smooth muscle cells significantly contribute to the initiation and progression of this pathology. Recently, we have demonstrated that elevated p...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1444759
更新日期:2018-12-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1865937
更新日期:2021-01-12 00:00:00
abstract::Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029206
更新日期:1996-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152630
更新日期:1978-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809002438
更新日期:1998-01-01 00:00:00
abstract::To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109005649
更新日期:2001-01-01 00:00:00
abstract::This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009152156
更新日期:1990-01-01 00:00:00
abstract::Purpose/Aim: Cementogenesis is a critical step in periodontal tissue regeneration given the essential role of cementum in anchoring teeth to the alveolar bone. This study is designed to achieve integrated cementum formation on the root surfaces of human teeth using growth factor-releasing scaffolds with periodontal li...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1191478
更新日期:2016-11-01 00:00:00
abstract::Cell cultures propagated from fetal bovine ligamentum nuchae were found to accumulate an extensive extracellular matrix containing collagen and elastic fibers when maintained for periods up to 8 weeks. The synthesis by these cells of two glycoproteins, designated MFP I and MFP II, which are immunologically related to ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152368
更新日期:1981-01-01 00:00:00
abstract::The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152378
更新日期:1981-01-01 00:00:00
abstract::Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923851
更新日期:2014-08-01 00:00:00
abstract::Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Genetic diseases affecting tooth structure have been classified by the tissue affected enamel versus dentin, and their pattern of inheritance autosomal dominant, autosomal recessive, or X-linked. Advances in molecular genetics and the Human Genome Project have provided substantial progress regarding the identification...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809019069
更新日期:1988-01-01 00:00:00
abstract::Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590935510
更新日期:2005-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590954131
更新日期:2005-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209409005633
更新日期:1994-01-01 00:00:00
abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909029111
更新日期:1999-01-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200390229912
更新日期:2003-01-01 00:00:00
abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490882628
更新日期:2004-01-01 00:00:00
abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000880
更新日期:2002-01-01 00:00:00
abstract::A high frequency restriction fragment length polymorphism (RFLP) at the 3'-end of the pigeon pro alpha 2(1) collagen gene was detected using the restriction endonuclease EcoR1. The distribution of this allelic variant was analyzed in DNA isolated from White Carneau pigeons genetically susceptible to the development of...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152437
更新日期:1991-01-01 00:00:00
abstract::Short term cultures were carried out with chondrocytes and tissue fragments from fetal guinea pig epiphyseal cartilage. Proteoglycans were isolated from these cultures and their properties were compared with those of proteoglycans from adult hyaline cartilage. It was concluded that the proteoglycans synthesized in cul...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152274
更新日期:1978-01-01 00:00:00