Abstract:
:Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture system (Pedrozo et al., J. Comp. Physiol. (A) 177:415-425). Inhibition of carbonic anhydrase by acetazolamide decreased statoconia production and volume, while inhibition of urease by acetohydroxamic acid reduced total statoconia number, but had no affect on statoconia volume. Inhibition of carbonic anhydrase initially increased and then decreased the statocyst pH, whereas inhibition of urease decreased statocyst pH at all times examined; simultaneous addition of both inhibitors also decreased pH. These effects were dose and time dependent. The results show that carbonic anhydrase and urease are required for statoconia formation and homeostasis, and for regulation of statocyst pH. This suggests that these two enzymes regulate mineralization at least partially through regulation of statocyst pH.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Pedrozo HA,Schwartz Z,Dean DD,Wiederhold ML,Boyan BDdoi
10.3109/03008209609029206subject
Has Abstractpub_date
1996-01-01 00:00:00pages
317-23issue
1-4eissn
0300-8207issn
1607-8438journal_volume
35pub_type
杂志文章abstract::The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152131
更新日期:1981-01-01 00:00:00
abstract::Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.546536
更新日期:2011-10-01 00:00:00
abstract::Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract:UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923875
更新日期:2014-08-01 00:00:00
abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1195826
更新日期:2016-09-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207709152610
更新日期:1977-01-01 00:00:00
abstract::Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009811
更新日期:1990-01-01 00:00:00
abstract::Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013716
更新日期:1995-01-01 00:00:00
abstract::Oral mandibular epithelium determines the position and number of tooth organs. Cranial neural crest-derived ectomesenchyme provides inductive signals for (i) tooth shape, and (ii) sequential determination and expression of several different epithelial-derived enamel proteins. Inductive signalling follows a precise seq...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008208909023878
更新日期:1989-01-01 00:00:00
abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023914
更新日期:1998-01-01 00:00:00
abstract::The small leucine-rich repeat proteoglycans (SLRPs) constitute a group of structurally and functionally related molecules that participate in the organization of the extracellular matrix ECM and have important effects on cell behavior. Osteosarcomas are heterogeneous bone tumors whose common characteristic is the prod...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200802147589
更新日期:2008-01-01 00:00:00
abstract::These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209409005633
更新日期:1994-01-01 00:00:00
abstract::The relationship between structural changes and hyaluronic acid (HA) content in muscular atrophy is unknown. The objective of our study was to conduct multifaceted evaluations of the change in muscle extensibility in muscular atrophy, in terms of morphological aspects as well as from a biochemical viewpoint, including...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590954131
更新日期:2005-01-01 00:00:00
abstract::Raman spectra have been determined for hyaluronan, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan monomers and aggregates. The nature of the saccharides and the pattern of sulfation can be discerned. There were only small spectral changes with pH and ionic composition. Differences between hydroxyl vibrations, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802398422
更新日期:2009-01-01 00:00:00
abstract::Purpose: In our previous study, we demonstrated that hyaluronan induces odontoblastic differentiation of dental pulp stem cells via interactions with CD44. However, it remains unclear whether CD44 expression by dental pulp stem cells is required for odontoblastic differentiation. Methods: We searched for a compound ot...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1865937
更新日期:2021-01-12 00:00:00
abstract::Hyaluronate lyase from streptococci group A was purified by chromatography on DEAE-Sephadex A-50 and on Biogel P-150, thereby enriching it about 1,000-fold and separating it into two enzyme fractions with the same amino acid composition. The photooxidation of hyaluronate lyase in the presence of methylene blue results...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207509152171
更新日期:1975-01-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenovirus...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490278142
更新日期:2004-01-01 00:00:00
abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000754
更新日期:2002-01-01 00:00:00
abstract::Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1751140
更新日期:2020-04-20 00:00:00
abstract::The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209309016835
更新日期:1993-01-01 00:00:00
abstract::Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase A...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709005621
更新日期:1987-01-01 00:00:00
abstract::The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609017468
更新日期:1986-01-01 00:00:00
abstract::Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809002438
更新日期:1998-01-01 00:00:00
abstract:PURPOSE:Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, whi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1276177
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1485666
更新日期:2018-09-01 00:00:00
abstract::DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152225
更新日期:1976-01-01 00:00:00
abstract::Tobacco smoking is considered a major risk factor for the development and progression of periodontal diseases (Haber, J. and Wattles, J. (1994). J. Periodontol., 64, 16-23). The purpose of this study was to determine the effects of nicotine on rat gingival fibroblasts (RGF) cultured in vitro. After ether anesthesia, r...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009005643
更新日期:2000-01-01 00:00:00