Regulation of statoconia mineralization in Aplysia californica in vitro.

Abstract:

:Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture system (Pedrozo et al., J. Comp. Physiol. (A) 177:415-425). Inhibition of carbonic anhydrase by acetazolamide decreased statoconia production and volume, while inhibition of urease by acetohydroxamic acid reduced total statoconia number, but had no affect on statoconia volume. Inhibition of carbonic anhydrase initially increased and then decreased the statocyst pH, whereas inhibition of urease decreased statocyst pH at all times examined; simultaneous addition of both inhibitors also decreased pH. These effects were dose and time dependent. The results show that carbonic anhydrase and urease are required for statoconia formation and homeostasis, and for regulation of statocyst pH. This suggests that these two enzymes regulate mineralization at least partially through regulation of statocyst pH.

journal_name

Connect Tissue Res

authors

Pedrozo HA,Schwartz Z,Dean DD,Wiederhold ML,Boyan BD

doi

10.3109/03008209609029206

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

317-23

issue

1-4

eissn

0300-8207

issn

1607-8438

journal_volume

35

pub_type

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