Abstract:
:The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipitating antibodies. Recent studies have focused on the possible role of elastolytic enzymes, particularly leukocyte elastase, in the pathogenesis of chronic obstructive lung disease, but there are no reports in the literature concerning the antigenic properties of peptides released by digestion of insoluble elastin with leukocyte elastase. In the present study, we have obtained antibodies in rabbits to peptides prepared by digestion of dog and human lung and aortic elastin with either oxalic acid or leukocyte elastase. By hemagglutination assays, we have shown generally that: (1) within a given species the peptides from aorta and lung cross-react strongly with one another, (2) there was rather poor cross-reactivity between peptides from different species (3) the peptides obtained by oxalic acid digestion cross-react poorly with those obtained by elastase digestion, (4) the antibodies to the peptides cross-reacted with the insoluble elastin from which the peptides were derived. These elastin-specific antibodies may be useful for ultrastructural localization of elastin and identification of fragments derived from elastin in the sera of humans and experimental animals.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Kucich U,Christner P,Rosenbloom J,Weinbaum Gdoi
10.3109/03008208109152131subject
Has Abstractpub_date
1981-01-01 00:00:00pages
121-6issue
2eissn
0300-8207issn
1607-8438journal_volume
8pub_type
杂志文章abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023914
更新日期:1998-01-01 00:00:00
abstract::Part of the hyaluronic acid (HA) synthesized in peripheral tissues enters the blood circulation through the lymph. It is rapidly taken up by the endothelial cells in the liver (half-life in blood is 2.5-5.5 minutes) and degraded. Pure primary cultures of liver endothelial cells were obtained by a newly developed techn...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609001971
更新日期:1986-01-01 00:00:00
abstract::Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium and mesenchyme, while Shh transcripts are present in dental epithelium only, suggesting that SHH functions as an a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013716
更新日期:1995-01-01 00:00:00
abstract::Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200390229912
更新日期:2003-01-01 00:00:00
abstract::Since cartilage is mainly an avascular tissue, chondrocytes exist in a low-level oxygen environment in vivo. In the present study, we investigated the effect of oxygen tension (20%, 5% and 1% gas phase oxygen concentrations) over a 20-day period on the extracellular matrix accumulation of bovine articular chondrocytes...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109014251
更新日期:2001-10-01 00:00:00
abstract::Periosteal explants obtained from rabbit calvariae are introduced as a model system to study contraction of soft connective tissue. Culturing of these explants resulted in a substantial time-dependent decrease of the surface area which coincided with a similar decrease in volume. A 75% reduction of the initial explant...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509028402
更新日期:1995-01-01 00:00:00
abstract::The efficacy of connective tissue explants is difficult to determine, particularly where autopsy material is required for research or clinical applications. We report here an optimised protocol using 5-chloromethylfluorescein diacetate (CMFDA) and ethidium homodimer-1 to distinguish viable and non-viable cells in a ra...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609028880
更新日期:1996-01-01 00:00:00
abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909029111
更新日期:1999-01-01 00:00:00
abstract::Hyperhomocysteinemia causes connective tissue pathology. Several theories on the mechanism of homocysteine toxicity in connective tissue are reviewed briefly. A possible new mechanism was revealed recently in the discovery of a reaction in which homocysteine thiolactone is converted to mercaptopropionaldehyde. The rea...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200801913742
更新日期:2008-01-01 00:00:00
abstract::Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth pl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1394295
更新日期:2018-01-01 00:00:00
abstract:AIM:To investigate the effect of extracellular matrix (ECM) proteins on characteristics of mesenchymal stem cells (MSCs) and tendon-derived cells (TDCs). MATERIALS AND METHODS:MSCs and TDCs, cultured in a monolayer (2D) or hydrogels (3D), with or without ECM protein supplementation, and on a non-viable native tendon (...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1215442
更新日期:2016-11-01 00:00:00
abstract::A theoretical expression has been derived for the mean collagen fibril length in tendon based on the assumption that collagen fibrils originate in cell surface invaginations and terminate either at some remote cell surface or another collagen fibril bundle. The expression thus determined requires knowledge of the effe...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909016814
更新日期:1989-01-01 00:00:00
abstract::An agarose gel matrix was utilized to grow chondrocytes from human donors of various ages in cell culture. The chondrocytes produced the pericellular matrix characteristic for such cells and synthesized collagen type II as well as glyco-saminoglycans. The latter exhibit the typical distribution pattern of the respecti...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609167140
更新日期:1986-01-01 00:00:00
abstract::Bone sialoprotein is a 34 kDa phosphorylated and sulphated glycoprotein that is essentially unique to mineralizing connective tissues. Recent studies on the developmental expression of BSP mRNA and the temporo-spatial appearance of the protein during bone formation in vivo and in vitro have demonstrated that BSP is ex...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029171
更新日期:1996-01-01 00:00:00
abstract::Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000880
更新日期:2002-01-01 00:00:00
abstract::The diameter of collagen fibrils has been measured in electron micrographs of the tunica media and adventitia of the common carotid artery of 14 rats (19,262 counts). The fibrils of the media were measured in each of its three interlamellar spaces. The mean fibril diameters in the innermost space and the middle space ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709002000
更新日期:1987-01-01 00:00:00
abstract::Objective: Aseptic loosening is a major problem in total joint replacement. Implant wear debris provokes a foreign body host response and activates cells to produce a variety of mediators and ROS, leading to periprosthetic osteolysis. Elevated ROS levels can harm proteasome function. Proteasome inhibitors have been re...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601186
更新日期:2019-11-01 00:00:00
abstract::Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Osteoarthritis (OA), the most common form of degenerative joint disease, is linked to high morbidity. It is predicted to be the single greatest cause of disability in the general population by 2030. The development of disease-modifying therapy for OA currently face great obstacle mainly because the onset and developme...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2016.1177036
更新日期:2016-07-01 00:00:00
abstract::The purpose of this study was to examine the hypoxic and inflammatory conditions after immobilization in the joint capsule of rat knees. The unilateral knee joints of adult male rats were immobilized with an internal fixator (Im group) for 1 day, 3 days, and 1, 2, 4, 8, and 16 weeks. Sham-operated animals had holes dr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.786056
更新日期:2013-01-01 00:00:00
abstract::Conditions were established to stimulate human gingival fibroblast explant cultures to synthesize milligram quantities of the metalloproteinase proenzymes, prostromelysin and procollagenase. To stimulate enzyme production, cells were treated with 1 nM recombinant human IL-1 beta for approximately 7 days under serum fr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009812
更新日期:1990-01-01 00:00:00
abstract::The ultrastructural features of the cells and of the oxytalan fibers within the periodontal ligaments of aged rats (2 year old) were quantified and compared with data for tissues obtained from younger animals (8 week old). Sections of the mid-root regions of the mandibular first molars were prepared for examination by...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509016978
更新日期:1995-01-01 00:00:00
abstract::This study evaluated the effect of short bouts of stretching on the soleus muscle after immobilization, by measuring the birefringence of the intramuscular connective tissue (ICT) and the muscle fiber area. Thirty rats were divided into five groups: the left soleus was immobilized in the shortened position; after immo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600995940
更新日期:2006-01-01 00:00:00
abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409013694
更新日期:1984-01-01 00:00:00
abstract::Purpose: Complications including arthrofibrosis have been reported after anterior cruciate ligament reconstruction (ACLR) even under accelerated rehabilitation. To overcome this, we developed an animal model of ACLR-induced arthrofibrosis without immobilization.Materials and Methods: Thirteen male Wistar rats were div...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1693548
更新日期:2019-11-26 00:00:00
abstract::Collagenolytic enzymes were quantitated by a method based on spectrophotometry of suspended reconstituted collagen fibrils. To obtain optically stable suspensions it was necessary to perform a short sonication of the aggregated fibrils at 10 degrees C. When fibrils were cleaved with mammalian fibroblast collagenase at...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152442
更新日期:1991-01-01 00:00:00
abstract::A detailed analysis of the D-period and axial electron density distribution of cornea and tendon collagen was carried out by means of X-ray diffraction and electron microscopy. Ultrastructural observations were made on replicas of freeze fractured and deep-etched specimens. Synchrotron radiation was used to obtain hig...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609001985
更新日期:1986-01-01 00:00:00
abstract::Two populations of primary osteoblasts and the cloned murine osteoblast cell line MC3T3 were activated with osteotropic agents and the conditioned media tested for the presence of macrophage colony stimulating factor (M-CSF). Differences in ability of the three populations to secrete the cytokine as well as in the eff...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023884
更新日期:1989-01-01 00:00:00