Abstract:
:A theoretical expression has been derived for the mean collagen fibril length in tendon based on the assumption that collagen fibrils originate in cell surface invaginations and terminate either at some remote cell surface or another collagen fibril bundle. The expression thus determined requires knowledge of the effective lengths of the fibrocytes (or fibrocyte assemblies) and the cellular content of the tendon. Both of these parameters have been measured experimentally as a function of age for rat-tail tendon using a combined light microscope and electron microscope approach. The results obtained for immature tendon suggest that the mean collagen fibril length is at least equal to the critical length required to maintain the appropriate tensile properties. In the most mature tissue studied, however, the mean-collagen fibril length is in excess of 100 times the critical length.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Craig AS,Birtles MJ,Conway JF,Parry DAdoi
10.3109/03008208909016814subject
Has Abstractpub_date
1989-01-01 00:00:00pages
51-62issue
1eissn
0300-8207issn
1607-8438journal_volume
19pub_type
杂志文章abstract::To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909005282
更新日期:1999-01-01 00:00:00
abstract::Oral mandibular epithelium determines the position and number of tooth organs. Cranial neural crest-derived ectomesenchyme provides inductive signals for (i) tooth shape, and (ii) sequential determination and expression of several different epithelial-derived enamel proteins. Inductive signalling follows a precise seq...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008208909023878
更新日期:1989-01-01 00:00:00
abstract::Purpose: Non-pathological child cortical bone (NPCCB) studies can provide clinicians with vital information and insights. However, assessing the anisotropic elastic properties of NPCCB remains a challenge for the biomechanical engineering community. For the first time, this paper provides elastic moduli values for NPC...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1570170
更新日期:2019-07-01 00:00:00
abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029217
更新日期:1996-01-01 00:00:00
abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152217
更新日期:1976-01-01 00:00:00
abstract::The molecular substructure of human articular cartilage has been difficult to study because of its complex composition and high degree of hydration. Using newly available small-angle X-ray diffraction (SAX) instrumentation that allows very short exposure times (0.1 to 10 sec), we have obtained spatially resolved infor...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The novel method of high voltage electron microscopic tomography (3D) has been applied for the first time to examine ultrastructural features and spatial relations between collagen fibrils and mineral crystals in a mouse mutant (oim/oim) which replicates a moderate to severe form of osteogenesis imperfecta. The animal...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010825
更新日期:1995-01-01 00:00:00
abstract::DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152225
更新日期:1976-01-01 00:00:00
abstract::The meniscal cartilages in the knee function to improve congruity of the medial and lateral femoro-tibial joints and play critical roles in load distribution and joint stability. Meniscal tears of various configurations are one of the most common conditions of the knee and are associated with an increased risk of deve...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2017.1284824
更新日期:2017-01-01 00:00:00
abstract::Bone sialoprotein is a 34 kDa phosphorylated and sulphated glycoprotein that is essentially unique to mineralizing connective tissues. Recent studies on the developmental expression of BSP mRNA and the temporo-spatial appearance of the protein during bone formation in vivo and in vitro have demonstrated that BSP is ex...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029171
更新日期:1996-01-01 00:00:00
abstract::Sonic hedgehog (Shh) expression is highly localized to the epithelium at the future sites of tooth development. This restricted expression suggests that inhibition of Shh in areas where teeth do not form may be an important mechanism in tooth germ localization. Recently, Hip, a putative vertebrate antagonist of Shh, h...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000907
更新日期:2002-01-01 00:00:00
abstract::To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290001023
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951440
更新日期:2014-10-01 00:00:00
abstract::The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309004860
更新日期:1983-01-01 00:00:00
abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009067666
更新日期:2000-01-01 00:00:00
abstract::The efficacy of connective tissue explants is difficult to determine, particularly where autopsy material is required for research or clinical applications. We report here an optimised protocol using 5-chloromethylfluorescein diacetate (CMFDA) and ethidium homodimer-1 to distinguish viable and non-viable cells in a ra...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609028880
更新日期:1996-01-01 00:00:00
abstract::Cartilage matrix degradation generates collagen type II fragments. The objective of this study is to explore the possibility that these collagen fragments may be part of an endogenous metabolic feedback. Initially, collagen fragments were extracted from normal or osteoarthritic cartilage, as part of a matrix fragment ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109014250
更新日期:2001-01-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are members of the Metzincin family of proteases responsible for degrading the extracellular matrix (ECM). In early studies, MMP degradation of the sub-epithelial basement membrane was thought to be tumor cell autonomous and contribute to the invasive behavior of malignant cells. It is...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2013.867339
更新日期:2014-01-01 00:00:00
abstract::Molecular distributions of the intermolecular cross-links in fetal bovine bone type I collagen fibrils were quantitatively determined and compared with those of periodontal ligament. Results indicated that Hyl and Lys residues in the COOH-terminal nonhelical peptide portions (residues 16C) of both alpha 1 chains were ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909050006
更新日期:1989-01-01 00:00:00
abstract::Objective: Aseptic loosening is a major problem in total joint replacement. Implant wear debris provokes a foreign body host response and activates cells to produce a variety of mediators and ROS, leading to periprosthetic osteolysis. Elevated ROS levels can harm proteasome function. Proteasome inhibitors have been re...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601186
更新日期:2019-11-01 00:00:00
abstract::We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309015011
更新日期:1983-01-01 00:00:00
abstract::Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207709152610
更新日期:1977-01-01 00:00:00
abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023914
更新日期:1998-01-01 00:00:00
abstract::Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152287
更新日期:1978-01-01 00:00:00
abstract::Primary chondrocytes cultured in agarose can escape the gel, accumulate at the interface between agarose and the culture medium, and form an outgrowing tissue. These outgrowths can appear as voluminous cartilage-like nodules that have never been previously investigated. In the present study, bovine articular chondrocy...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200903281683
更新日期:2010-06-01 00:00:00
abstract::Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1759562
更新日期:2020-05-05 00:00:00
abstract::Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609028895
更新日期:1996-01-01 00:00:00
abstract::Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium and mesenchyme, while Shh transcripts are present in dental epithelium only, suggesting that SHH functions as an a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:Treatment of meniscal injury is important for osteoarthritis (OA) prevention. Meniscus cells are divided between inner and outer cells, which have different characteristics and vascularity. We evaluated the effects of hyaluronic acid (HA) on the proliferation and migration of human inner and outer meniscus cell...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1465053
更新日期:2019-03-01 00:00:00