Abstract:
:Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in the bottom fraction (dA1) of a CsCl gradient after ultracentrifugation in associative buffer (0.5 M guanidine X HCl). The incorporated [35S]-sulfate in fraction dA1 from the culture medium eluted in two peaks with average partition coefficients (Kav) of 0.14 and 0.45 respectively, on Sepharose CL-2B eluted with dissociative buffer (4 M guanidine X HCl). A significant percentage of incorporated [35S]-sulfate was found in the medium dA4 fraction (44%). The Kav of this fraction on Sepharose CL-2B was 0.66 with a shoulder of incorporated [35S]-sulfate at Kav, 0.22. In contrast to the culture medium, cellular CsCl gradient fractions dA1-dA3 showed Kav's on Sepharose CL-2B ranging from 0.63-0.75. Cellular fraction dA4 was even more polydisperse. A dD1 fraction (proteoglycan monomer) prepared by CsCl ultracentrifugation in dissociative buffer of [35S]-sulfate labelled culture medium eluted with a Kav of 0.25 on Sepharose CL-2B identical to the Kav of bovine nasal cartilage A1D1 and human tissue osteophyte A1D1 chromatographed under identical conditions. Glycosaminoglycan analysis demonstrated significant amounts of chondroitin 6- and 4-sulfate in unfractionated culture medium and in those proteoglycan fractions generated from culture medium (dA1, dA2 and dD1). In contrast, cellular fractions dA1-dA3 and medium fraction dA4 were enriched in dermatan sulfate. The size of the [35S]-sulfated glycosaminoglycan chains analyzed by Sepharose CL-6B chromatography showed considerable polydispersity (Kav range, 0.29-0.52). The results of this study indicated that cells derived from the cartilaginous component of human osteophyte synthesized several distinct populations of sulfated proteoglycans. These results may reflect the heterogeneity of cells which grow out from osteophyte organ explants and become established in monolayer culture.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Malemud CJ,Moskowitz RW,Goldberg VMdoi
10.3109/03008208409013694subject
Has Abstractpub_date
1984-01-01 00:00:00pages
319-35issue
3-4eissn
0300-8207issn
1607-8438journal_volume
12pub_type
杂志文章abstract::STR/ort mice develop a naturally occurring osteoarthritis (OA) of the knee joints. However, the evaluation of early OA changes has been difficult due to variability caused by gender, individual differences, and differences between the right and left lower limbs. The objective of this study was to analyze the variabili...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200902836065
更新日期:2009-01-01 00:00:00
abstract::The fibrous collagens form the structural basis of all mammalian connective tissues, including the vasculature, dermis, bones, tendons, cartilage, and those tissues that support organs such as the heart, kidneys, liver, and lungs. The helical structure of collagen has been extensively studied but in addition to its he...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2010.511353
更新日期:2011-02-01 00:00:00
abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923865
更新日期:2014-08-01 00:00:00
abstract::Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1702031
更新日期:2020-05-01 00:00:00
abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000754
更新日期:2002-01-01 00:00:00
abstract::Hyperhomocysteinemia causes connective tissue pathology. Several theories on the mechanism of homocysteine toxicity in connective tissue are reviewed briefly. A possible new mechanism was revealed recently in the discovery of a reaction in which homocysteine thiolactone is converted to mercaptopropionaldehyde. The rea...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152217
更新日期:1976-01-01 00:00:00
abstract::Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029206
更新日期:1996-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1276177
更新日期:2017-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1195826
更新日期:2016-09-01 00:00:00
abstract::These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209409005633
更新日期:1994-01-01 00:00:00
abstract::The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109016840
更新日期:2001-01-01 00:00:00
abstract::Tobacco smoking is considered a major risk factor for the development and progression of periodontal diseases (Haber, J. and Wattles, J. (1994). J. Periodontol., 64, 16-23). The purpose of this study was to determine the effects of nicotine on rat gingival fibroblasts (RGF) cultured in vitro. After ether anesthesia, r...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009005643
更新日期:2000-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590954131
更新日期:2005-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509116832
更新日期:1995-01-01 00:00:00
abstract::We have investigated the changes in some of the biochemical and biophysical properties of human femoral head cartilage on the one hand during ageing and on the other hand in osteoarthritis. Topographical variations were also investigated. The parameters studied were those relevant to cartilage function, viz., proteogl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909043895
更新日期:1989-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200903318295
更新日期:2010-08-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152131
更新日期:1981-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601186
更新日期:2019-11-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909002417
更新日期:1989-01-01 00:00:00
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journal_title:Connective tissue research
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更新日期:2010-12-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.524719
更新日期:2011-01-01 00:00:00
abstract::The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309004860
更新日期:1983-01-01 00:00:00
abstract::The molecular substructure of human articular cartilage has been difficult to study because of its complex composition and high degree of hydration. Using newly available small-angle X-ray diffraction (SAX) instrumentation that allows very short exposure times (0.1 to 10 sec), we have obtained spatially resolved infor...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802143141
更新日期:2008-01-01 00:00:00
abstract::Glycosaminoglycan synthesis by cells cultured from nodule or band tissues of patients with Dupuytren's disease was compared with that of skin fibroblasts cultured from uninvolved areas of the palm. No differences were observed in culture which could account either for the abnormal glycosaminoglycan content or the incr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160272
更新日期:1982-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209209016818
更新日期:1992-01-01 00:00:00
abstract::The effect of I- and C1- on the enzymatic degradation of elastin pancreatic elastase has been studied. At low halide concentrations both ions show a marked stimulation of elastolysis, I- being significantly more effective than Cl-. Kinetic plots of enzyme activity indicate that anions enhance the susceptibility of the...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152616
更新日期:1978-01-01 00:00:00
abstract::Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular pro...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209009152421
更新日期:1990-01-01 00:00:00