Abstract:
:Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has been mainly realized in these systems. In order to set up methods for mRNA investigation, in situ hybridization of calbindin-D28k mRNAs was performed in the continuously erupting incisor of Sprague-Dawley rats (15-, 30-, and 56-day-old). 35S UTP labelled antisense and sense riboprobes specific for brain calbindin-D 28k were used for in situ hybridization. Specific and non-specific signals could not be discerned when studying decalcified samples. In contrast, on sections not pretreated with EDTA, calbindin-D 28k transcripts (in tooth and kidney) appeared strongly labelled with antisense probes, while sense probes provided a negligible background. In ameloblasts, the signal (i.e., calbindin-D 28k mRNA levels) increased during the presecretory stage. Different mRNA gradients and subcellular distribution patterns characterized the secretory and maturation stages. A nuclear labelling was observed, associated with the highest levels of transcripts. These data suggest a developmental control of calbindin-D28k mRNA transcription. Calbindin-D28k gene expression appears to be up-regulated during the initiation of both secretory and maturation stages of enamel mineralization.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Hotton D,Davideau JL,Bernaudin JF,Berdal Adoi
10.3109/03008209509013716subject
Has Abstractpub_date
1995-01-01 00:00:00pages
137-43issue
1-4eissn
0300-8207issn
1607-8438journal_volume
32pub_type
杂志文章abstract::Although hyaluronate is not a boundary lubricant in cartilaginous and latex:glass bearings, a distinct interaction with purified synovial lubricating factor (PSLF) was demonstrated by three means: 1) enhancement of lubricating ability in an artificial test system; 2) viscometry; 3) electron microscopy. The interaction...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209209016818
更新日期:1992-01-01 00:00:00
abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017041
更新日期:1998-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809008059
更新日期:1988-01-01 00:00:00
abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909029111
更新日期:1999-01-01 00:00:00
abstract::Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular pro...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209009152421
更新日期:1990-01-01 00:00:00
abstract::This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovarie...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200902855917
更新日期:2010-01-01 00:00:00
abstract::Proteoglycans and hyaluronic acid synthesized by human skin fibroblasts in culture were characterized, and the effect of thyroid hormone deficiency was examined. The fibroblasts in culture synthesize hyaluronic acid, dermatan sulfate (DS) proteoglycans and heparan sulfate (HS) proteoglycans. Hyaluronic acid is almost ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809015024
更新日期:1988-01-01 00:00:00
abstract::Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802684615
更新日期:2009-01-01 00:00:00
abstract::Cell cultures propagated from fetal bovine ligamentum nuchae were found to accumulate an extensive extracellular matrix containing collagen and elastic fibers when maintained for periods up to 8 weeks. The synthesis by these cells of two glycoproteins, designated MFP I and MFP II, which are immunologically related to ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152368
更新日期:1981-01-01 00:00:00
abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209309016835
更新日期:1993-01-01 00:00:00
abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010814
更新日期:1995-01-01 00:00:00
abstract::To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of la...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709001994
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951440
更新日期:2014-10-01 00:00:00
abstract::The effect of intra-bone injection of differentiated rat bone marrow mesenchymal stem cells (BMMSCs) into the femur of osteoporotic female rats was studied. Osteoporosis was induced in Wistar female rats by bilateral ovariectomy. Then, 0.75 million BMMSCs isolated from healthy rats were injected into the femurs of ost...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008201003597049
更新日期:2010-12-01 00:00:00
abstract::Cumulative damage to long-lived connective tissue proteins play a key role in the development of age-related human diseases such as cardiovascular stiffening and age-related macular degeneration. The processes that result in the accumulation of increasingly insoluble, undigestible damaged collagen are only partially k...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600721569
更新日期:2006-01-01 00:00:00
abstract::These studies examined the effects of heat-inactivated horse serum, insulin, triiodothyronine (T3), and thyroxine (T4), individually and in combination, on collagen and proteoglycan synthesis by primary cell cultures of articular chondrocytes from immature male rabbits. Insulin concentrations of 25 to 100 ng/ml (4.4 t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209409005633
更新日期:1994-01-01 00:00:00
abstract::Fras1 and the structurally related proteins Frem1, Frem2, and Frem3, comprise a novel family of extracellular matrix proteins, which localize in a similar fashion underneath the lamina densa of epithelial basement membranes. They are involved in the structural adhesion of the skin epithelium to its underlying mesenchy...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802148025
更新日期:2008-01-01 00:00:00
abstract::Since cartilage is mainly an avascular tissue, chondrocytes exist in a low-level oxygen environment in vivo. In the present study, we investigated the effect of oxygen tension (20%, 5% and 1% gas phase oxygen concentrations) over a 20-day period on the extracellular matrix accumulation of bovine articular chondrocytes...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109014251
更新日期:2001-10-01 00:00:00
abstract::Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152287
更新日期:1978-01-01 00:00:00
abstract::Bovine articular chondrocytes cultured in agarose gel comprise a heterogeneous population when judged by morphological and histochemical criteria. The purpose of the present experiments was to compare, under the same conditions of culture, sub-populations of chondrocytes derived from different depths of articular cart...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809016808
更新日期:1988-01-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009811
更新日期:1990-01-01 00:00:00
abstract::The resorption of devitalized bone by isolated osteoclasts in culture was studied by scanning electron microscopy. Osteoclasts attached to the bone and resorbed mineral but left most of matrix collagen undigested in the resorption pits. Postculture-treatment of bone substrate with collagenase completely removed the ma...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023885
更新日期:1989-01-01 00:00:00
abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1195826
更新日期:2016-09-01 00:00:00
abstract::The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1839436
更新日期:2020-12-11 00:00:00
abstract::The total and individual glycosaminoglycan (GAG) content at various stages of the Dupuytren disease process and in samples of normal palmar connective tissue (palmar dermis, palmar fascia and digital flexor tendon) from the hands of uninvolved age-matched controls have been assayed and compared. Morphological comparis...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160258
更新日期:1982-01-01 00:00:00
abstract::Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1439482
更新日期:2018-11-01 00:00:00
abstract::Objective: Aseptic loosening is a major problem in total joint replacement. Implant wear debris provokes a foreign body host response and activates cells to produce a variety of mediators and ROS, leading to periprosthetic osteolysis. Elevated ROS levels can harm proteasome function. Proteasome inhibitors have been re...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601186
更新日期:2019-11-01 00:00:00