Components of increased labelling with putrescine and fucose during healing of skin wounds.

Abstract:

:To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of label which was greatest at 3 days. Incubation for various times suggests that the incorporation of fucose and proline is dependent on protein synthesis, whereas putrescine is incorporated into preformed proteins. Putrescine and fucose label polypeptides with an Mr of about 45,000 before and 14,000 after reduction. These correspond in size with the aminopropeptide of type III collagen. Other labelled material of higher molecular weight is partly degraded to similar polypeptides on collagenase digestion. Much of the [3H]putrescine in the polypeptides is in the form of gamma-glutamyl putrescine. It is hypothesized that isopeptide linkage of the aminopropeptide III occurs in wound healing.

journal_name

Connect Tissue Res

authors

Bowness JM,Henteleff H,Dolynchuk KN

doi

10.3109/03008208709001994

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

57-70

issue

1

eissn

0300-8207

issn

1607-8438

journal_volume

16

pub_type

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