Abstract:
:Bone and dentin contain many phosphoproteins in their extracellular matrix. It has been postulated that the anionic character of these proteins, and the presence of phosphate groups in particular, is important for various functions related to biomineralization. Phosphophoryns (PP), are the most highly phosphorylated dentin matrix components. However, the tissue form of PP can be further phosphorylated in vitro by cytosolic and membrane associated-endogenous messenger-independent kinases from osteoblast-like cells. To examine the kinases, a 2-dimensional zymogram technique has been developed for the detection of casein kinase II (CKII) activity of purified kinases using intact PP as the substrate. After isolation by subcellular fractionation and ion-exchange chromatographic techniques, the enzymes are electrophoresed on an isoelectric focusing gel in the absence of SDS and disulfide bond breaking reagents. This first dimension gel is then layered on the zymogram gel containing PP + SDS. After electrophoresis, the gels are incubated in 250-500 microCi [gamma-32P] ATP. Autoradiography then detects kinase activity. Comparison of the UMR 106 CKII cytosolic and membrane-bound fractions showed that they were different in M(r) and focusing pH, suggesting the presence of CKII isoforms. CKI, which could phosphorylate the native PP in vitro, could not phosphorylate a dephosphorylated preparation (dPP). However, if the dPP was first exposed to CKII and unlabeled ATP then reacted with CKI and [gamma-32P]ATP, the PP was phosphorylated. This prerequisite phosphorylation by CKII indicates that in vivo PP phosphorylation probably occurs in a series of regulated steps as a co- or post-translational process.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Sfeir C,Veis Adoi
10.3109/03008209609029194subject
Has Abstractpub_date
1996-01-01 00:00:00pages
215-22issue
1-4eissn
0300-8207issn
1607-8438journal_volume
35pub_type
杂志文章abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029217
更新日期:1996-01-01 00:00:00
abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017041
更新日期:1998-01-01 00:00:00
abstract::Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collage...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200601143508
更新日期:2007-01-01 00:00:00
abstract::The paper reviews the changes in ameloblast ultrastructure, concomitant with the changes in its functions across the major stages of amelogenesis. It describes the mechanisms associated with the major events in biosynthesis and degradation of the major enamel proteins (amelogenins and tuftelin/enamelins) and with the ...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509013710
更新日期:1995-01-01 00:00:00
abstract::The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152627
更新日期:1978-01-01 00:00:00
abstract::Purpose: Complications including arthrofibrosis have been reported after anterior cruciate ligament reconstruction (ACLR) even under accelerated rehabilitation. To overcome this, we developed an animal model of ACLR-induced arthrofibrosis without immobilization.Materials and Methods: Thirteen male Wistar rats were div...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1693548
更新日期:2019-11-26 00:00:00
abstract::Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.996702
更新日期:2015-01-01 00:00:00
abstract::Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152629
更新日期:1978-01-01 00:00:00
abstract::Osteogenesis imperfecta is a congenital disease commonly characterized by brittle bones and caused by mutations in the genes encoding Type I collagen, the single most abundant protein produced by the body. The oim model has a natural collagen mutation, converting its heterotrimeric structure (two α1 and one α2 chains)...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923860
更新日期:2014-08-01 00:00:00
abstract::The thermal stability and solubility profiles of human hypertrophic scar collagen have been measured and compared with those of the collagens present in human normal scar, human normal skin and a lathyritic dermal tissue. The thermal stability profiles of the hypertrophic scar collagens were found to be very similar t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409152138
更新日期:1984-01-01 00:00:00
abstract::Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are bei...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2018.1517759
更新日期:2019-01-01 00:00:00
abstract::The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802684615
更新日期:2009-01-01 00:00:00
abstract::Ligaments have limited regenerative potential and as a consequence, repair is protracted and results in a mechanically inferior tissue more scar-like than native ligament. We previously reported that a single injection of interleukin-1 receptor antagonist (IL-1Ra) delivered at the time of injury, decreased the number ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.906408
更新日期:2014-06-01 00:00:00
abstract::Bovine articular chondrocytes cultured in agarose gel comprise a heterogeneous population when judged by morphological and histochemical criteria. The purpose of the present experiments was to compare, under the same conditions of culture, sub-populations of chondrocytes derived from different depths of articular cart...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809016808
更新日期:1988-01-01 00:00:00
abstract::Tobacco smoking is considered a major risk factor for the development and progression of periodontal diseases (Haber, J. and Wattles, J. (1994). J. Periodontol., 64, 16-23). The purpose of this study was to determine the effects of nicotine on rat gingival fibroblasts (RGF) cultured in vitro. After ether anesthesia, r...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009005643
更新日期:2000-01-01 00:00:00
abstract::Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.546536
更新日期:2011-10-01 00:00:00
abstract::This study aims to elucidate the feasible geometry of the scaffolds in bone and periodontal tissue engineering. Several biomaterials with different geometries are compared in terms of their patterns of ectopic BMP-induced chondrogenesis and osteogenesis. The materials include a honeycomb-shaped hydroxyapatite (HCHAP) ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290001104
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:Treatment of meniscal injury is important for osteoarthritis (OA) prevention. Meniscus cells are divided between inner and outer cells, which have different characteristics and vascularity. We evaluated the effects of hyaluronic acid (HA) on the proliferation and migration of human inner and outer meniscus cell...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1465053
更新日期:2019-03-01 00:00:00
abstract::Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth pl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1394295
更新日期:2018-01-01 00:00:00
abstract::Short term cultures were carried out with chondrocytes and tissue fragments from fetal guinea pig epiphyseal cartilage. Proteoglycans were isolated from these cultures and their properties were compared with those of proteoglycans from adult hyaline cartilage. It was concluded that the proteoglycans synthesized in cul...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152274
更新日期:1978-01-01 00:00:00
abstract::Volume and morphology of chondrocytes in osteoarthritic human hip joint articular cartilage were characterized, and their relationship to tissue structure and function was determined. Human osteochondral articular cartilage samples (n=16) were obtained from the femoral heads of nine patients undergoing total hip arthr...
journal_title:Connective tissue research
pub_type: 临床试验,杂志文章
doi:10.3109/03008207.2014.912645
更新日期:2014-08-01 00:00:00
abstract::A transmission electron microscope study of carbonate apatite crystals isolated from bone and mineralizing tendon, as well as those produced synthetically under approximated-physiological conditions, shows that they are thin irregular shaped plates. Electron diffraction patterns of individual crystals confirm that the...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109029158
更新日期:1991-01-01 00:00:00
abstract::Size measurements of dispersed rat bone crystals show that with age a greater proportion exceeds 400 A in length. The surface fractures of more heavily mineralized bones tend to be smooth and stepped, whereas those of less mineralized bones are fibrous. These observations combined with information reported elsewhere o...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909050015
更新日期:1989-01-01 00:00:00
abstract::To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290001023
更新日期:2002-01-01 00:00:00
abstract::Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium and mesenchyme, while Shh transcripts are present in dental epithelium only, suggesting that SHH functions as an a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::In the absence of ascorbic acid, confluent human skin fibroblasts incubated in 0.5% serum-supplemented medium had one-third of the level of lysyl hydroxylase activity of cells incubated in media containing high serum concentrations (5-20%). This difference appeared to be due to a decline in the enzyme activity followi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509152396
更新日期:1985-01-01 00:00:00
abstract::Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TI...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160222
更新日期:1997-01-01 00:00:00
abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152166
更新日期:1991-01-01 00:00:00
abstract::The effect of intra-bone injection of differentiated rat bone marrow mesenchymal stem cells (BMMSCs) into the femur of osteoporotic female rats was studied. Osteoporosis was induced in Wistar female rats by bilateral ovariectomy. Then, 0.75 million BMMSCs isolated from healthy rats were injected into the femurs of ost...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008201003597049
更新日期:2010-12-01 00:00:00