Abstract:
:This study aims to elucidate the feasible geometry of the scaffolds in bone and periodontal tissue engineering. Several biomaterials with different geometries are compared in terms of their patterns of ectopic BMP-induced chondrogenesis and osteogenesis. The materials include a honeycomb-shaped hydroxyapatite (HCHAP) with different tunnel sizes, a laser-perforated collagen membrane (LPM), and CPSA bioglass fibers. Implanted pellets were removed at 1-4 weeks and analyzed for bone and cartilage formation histologically and biochemically. Porous particles of hydroxyapatite (PPHAP), porous blocks of hydroxyapatite (PBHAP), and LPM did not induce detectable cartilage formation. In straight tunnel structures with various diameters in honeycomb-shaped hydroxyapatite (HCHAP), tunnels with smaller diameters (approximately 0.1 mm) induced cartilage followed by bone formation, while one with a larger diameter (0.35 mm) directly induced bone formation within the tunnels. It is concluded that the "vasculature-inducing geometry" of the carrier as an ECM is crucially important for osteogenesis.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Kuboki Y,Jin Q,Kikuchi M,Mamood J,Takita Hdoi
10.1080/03008200290001104keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
529-34issue
2-3eissn
0300-8207issn
1607-8438journal_volume
43pub_type
杂志文章abstract::In the absence of ascorbic acid, confluent human skin fibroblasts incubated in 0.5% serum-supplemented medium had one-third of the level of lysyl hydroxylase activity of cells incubated in media containing high serum concentrations (5-20%). This difference appeared to be due to a decline in the enzyme activity followi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509152396
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abstract::We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody...
journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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journal_title:Connective tissue research
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更新日期:1995-01-01 00:00:00
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journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00