Geometry of artificial ECM: sizes of pores controlling phenotype expression in BMP-induced osteogenesis and chondrogenesis.

Abstract:

:This study aims to elucidate the feasible geometry of the scaffolds in bone and periodontal tissue engineering. Several biomaterials with different geometries are compared in terms of their patterns of ectopic BMP-induced chondrogenesis and osteogenesis. The materials include a honeycomb-shaped hydroxyapatite (HCHAP) with different tunnel sizes, a laser-perforated collagen membrane (LPM), and CPSA bioglass fibers. Implanted pellets were removed at 1-4 weeks and analyzed for bone and cartilage formation histologically and biochemically. Porous particles of hydroxyapatite (PPHAP), porous blocks of hydroxyapatite (PBHAP), and LPM did not induce detectable cartilage formation. In straight tunnel structures with various diameters in honeycomb-shaped hydroxyapatite (HCHAP), tunnels with smaller diameters (approximately 0.1 mm) induced cartilage followed by bone formation, while one with a larger diameter (0.35 mm) directly induced bone formation within the tunnels. It is concluded that the "vasculature-inducing geometry" of the carrier as an ECM is crucially important for osteogenesis.

journal_name

Connect Tissue Res

authors

Kuboki Y,Jin Q,Kikuchi M,Mamood J,Takita H

doi

10.1080/03008200290001104

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

529-34

issue

2-3

eissn

0300-8207

issn

1607-8438

journal_volume

43

pub_type

杂志文章
  • Serum stimulation of lysyl hydroxylase activity in cultured human skin fibroblasts.

    abstract::In the absence of ascorbic acid, confluent human skin fibroblasts incubated in 0.5% serum-supplemented medium had one-third of the level of lysyl hydroxylase activity of cells incubated in media containing high serum concentrations (5-20%). This difference appeared to be due to a decline in the enzyme activity followi...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208509152396

    authors: Murad S,Sivarajah A,Pinnell SR

    更新日期:1985-01-01 00:00:00

  • Collagenase production at the border of granulation tissue in a healing wound: macrophage and mesenchymal collagenase production in vivo.

    abstract::We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109006995

    authors: Porras-Reyes BH,Blair HC,Jeffrey JJ,Mustoe TA

    更新日期:1991-01-01 00:00:00

  • Explant models for meniscus metabolism, injury, repair, and healing.

    abstract::Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2019.1702031

    authors: Tarafder S,Park G,Lee CH

    更新日期:2020-05-01 00:00:00

  • Ovalbumin is a component of the chicken eggshell matrix.

    abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509010814

    authors: Hincke MT

    更新日期:1995-01-01 00:00:00

  • Effect of oxygen tension on chondrocyte extracellular matrix accumulation.

    abstract::Since cartilage is mainly an avascular tissue, chondrocytes exist in a low-level oxygen environment in vivo. In the present study, we investigated the effect of oxygen tension (20%, 5% and 1% gas phase oxygen concentrations) over a 20-day period on the extracellular matrix accumulation of bovine articular chondrocytes...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200109014251

    authors: Murphy CL,Sambanis A

    更新日期:2001-10-01 00:00:00

  • Aortic smooth muscle cells migration and the role of metalloproteinases and hyaluronan.

    abstract::Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200802143141

    authors: Vigetti D,Moretto P,Viola M,Genasetti A,Rizzi M,Karousou E,Clerici M,Bartolini B,Pallotti F,De Luca G,Passi A

    更新日期:2008-01-01 00:00:00

  • Coordinate regulation of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expression in human vascular smooth muscle cells.

    abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200009067666

    authors: Kato S,Yasukawa H,Fujii T,Yamaguchi M,Miyagi N,Okamoto K,Wada Y,Miyamoto T,Morimatsu M,Fox JC

    更新日期:2000-01-01 00:00:00

  • Adenovirus-mediated gene transfer of growth and differentiation factor-5 into tenocytes and the healing rat Achilles tendon.

    abstract::Growth and differentiation factor-5 (GDF-5) is known to induce tendon tissue and stimulate tendon healing. The hypothesis was that adenoviral GDF-5 transfer leads to transitory transgene expression and improves Achilles tendon healing. In vitro experiments were first performed with rat tenocytes. Transgene expression ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200500237120

    authors: Rickert M,Wang H,Wieloch P,Lorenz H,Steck E,Sabo D,Richter W

    更新日期:2005-01-01 00:00:00

  • Initial characterization of small proteoglycans synthesized by embryonic chick leg muscle-associated connective tissues.

    abstract::Muscle development in the embryonic chick involves the interfacing of two different cell types: myogenic cells forming the contractile network and connective tissue cells which wrap this network into discrete morphologies. Previous reports from this laboratory detailed the characterization of large extracellular matri...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809015023

    authors: Young HE,Carrino DA,Caplan AI

    更新日期:1988-01-01 00:00:00

  • Depolymerization and de-N-acetylation of glycosaminoglycuronans by the action of alkali in the presence of sodium borohydride.

    abstract::Depolymerization of Ch-4-S, Ch-6-S, HA and HP was observed by gel filtration after treatment with 2-10 N NaOH or Ba(OH)2 in the presence of NaBH4 at 80-83 degrees for 1-5 hr. Depolymerized products were isolated after these treatments. 4-Deoxy-alpha-L-threo-4-enohexopyranosyluronic acid residue as the nonreducing end ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207509152343

    authors: Hirano S,Ishigami M,Koga Y

    更新日期:1975-01-01 00:00:00

  • Characterization of a porcine amelogenin preparation, EMDOGAIN, a biological treatment for periodontal disease.

    abstract::EMDOGAIN is derived from porcine developing enamel matrix and has been shown to facilitate regeneration of the periodontium, although its mechanism of action is unknown. The aim of the present study was to identify enamel matrix proteins and proteolytic enzymes present in EMDOGAIN and compare them with those extracted...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290000880

    authors: Maycock J,Wood SR,Brookes SJ,Shore RC,Robinson C,Kirkham J

    更新日期:2002-01-01 00:00:00

  • TNF-α and TGF-β1 regulate Syndecan-4 expression in nucleus pulposus cells: role of the mitogen-activated protein kinase and NF-κB pathways.

    abstract::Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorl...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2014.996702

    authors: Yang H,Liu H,Li X,Pan H,Li Z,Wang J,Zheng Z

    更新日期:2015-01-01 00:00:00

  • The physical properties of elastic fibers and elastolysis.

    abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207609152217

    authors: Hall DA

    更新日期:1976-01-01 00:00:00

  • The effects of collagen fragments on the extracellular matrix metabolism of bovine and human chondrocytes.

    abstract::Cartilage matrix degradation generates collagen type II fragments. The objective of this study is to explore the possibility that these collagen fragments may be part of an endogenous metabolic feedback. Initially, collagen fragments were extracted from normal or osteoarthritic cartilage, as part of a matrix fragment ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200109014250

    authors: Jennings L,Wu L,King KB,Hämmerle H,Cs-Szabo G,Mollenhauer J

    更新日期:2001-01-01 00:00:00

  • Progenitor cells from dental follicle are able to form cementum matrix in vivo.

    abstract::To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200290001023

    authors: Handa K,Saito M,Tsunoda A,Yamauchi M,Hattori S,Sato S,Toyoda M,Teranaka T,Narayanan AS

    更新日期:2002-01-01 00:00:00

  • Healing of biodegradable vascular prosthesis. Incorporation of 3H-valine into proteins in the subendothelial scar and host intima-media of rat aorta.

    abstract::Heparin treated and aldehyde crosslinked rat aorta segments were implanted in infrarenal aorta of homologous rats. One year following aortic replacement, the subendothelial scar and the prosthetic remnants were excised. The scar and the host intima-media were incubated with 3H-valine for 4 h and extracted with 5 M gua...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208309005609

    authors: Moczar M,Bessou JP,Loisance D

    更新日期:1983-01-01 00:00:00

  • Studies on the mechanism of hyaluronate lyase action.

    abstract::Hyaluronate lyase from streptococci group A was purified by chromatography on DEAE-Sephadex A-50 and on Biogel P-150, thereby enriching it about 1,000-fold and separating it into two enzyme fractions with the same amino acid composition. The photooxidation of hyaluronate lyase in the presence of methylene blue results...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207509152171

    authors: Greiling H,Stuhlsatz HW,Eberhard T,Eberhard A

    更新日期:1975-01-01 00:00:00

  • Ectopic osteogenesis of hBMP-2 gene-transduced human bone mesenchymal stem cells/BCB.

    abstract::We determined the feasibility of using scaffolds of adenoviral human BMP2 gene (AdBMP2)-modified human bone marrow mesenchymal stem cells (hBMSCs) and antigen-free bovine cancellous bone (BCB) to construct bone tissue. hMSCs were infected with AdBMP-2. Expression of BMP-2 and alkaline phosphatase confirmed successful ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200903318295

    authors: Han D,Li J,Guan X

    更新日期:2010-08-01 00:00:00

  • Interactions of elastin and microfibrils in elastogenesis of human pulmonary fibroblasts in culture.

    abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209309016835

    authors: Fukuda Y,Nakazawa N,Yamanaka N

    更新日期:1993-01-01 00:00:00

  • Bio-ink development for three-dimensional bioprinting of hetero-cellular cartilage constructs.

    abstract::Bioprinting is a promising tool to fabricate organized cartilage. This study aimed to investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), and to explore (zone-specific) chondrogenesis of chondrocytes, articular cartilage progen...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2018.1553960

    authors: Mouser VHM,Levato R,Mensinga A,Dhert WJA,Gawlitta D,Malda J

    更新日期:2020-03-01 00:00:00

  • Partial-width injuries of the rat rotator cuff heal with fibrosis.

    abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2018.1485666

    authors: Lemmon EA,Locke RC,Szostek AK,Ganji E,Killian ML

    更新日期:2018-09-01 00:00:00

  • Normalization of the tumor microenvironment: evidence for tissue inhibitor of metalloproteinase-2 as a cancer therapeutic.

    abstract::Matrix metalloproteinases (MMPs) are members of the Metzincin family of proteases responsible for degrading the extracellular matrix (ECM). In early studies, MMP degradation of the sub-epithelial basement membrane was thought to be tumor cell autonomous and contribute to the invasive behavior of malignant cells. It is...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.3109/03008207.2013.867339

    authors: Stetler-Stevenson WG,Gavil NV

    更新日期:2014-01-01 00:00:00

  • Immunoelectron microscopy of proteodermatan sulfate in human mid-dermis.

    abstract::Proteodermatan sulfate was isolated from the skin of human, female breast in 6-M urea and proteolytic inhibitors at 70 degrees C and purified on Sephacryl S-200. It was composed of 55% protein and 45% dermatan sulfate, displayed one protein and carbohydrate-stainable band on agarose-polyacrylamide gels, yielded dermat...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208509152390

    authors: Longas MO,Fleischmajer R

    更新日期:1985-01-01 00:00:00

  • Downregulation of decorin and transforming growth factor-beta1 by decorin gene suppression in tendinocytes.

    abstract::Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by co...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200590935510

    authors: Hosaka Y,Kirisawa R,Mafune N,Takehana K

    更新日期:2005-01-01 00:00:00

  • Is low molecular weight hyaluronan an early indicator of disease in avian systemic sclerosis?

    abstract:AIM OF THE STUDY:To further elucidate the pathogenesis of systemic sclerosis (SSc) an experimental avian model was used. The University of California at Davis line 200 (UCD-200) chickens spontaneously develop a SSc-like disease that has most features of human SSc with vascular effects, inflammation, autoimmunity, and f...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2016.1182997

    authors: Kerje S,Hellman U,Do L,Larsson G,Kämpe O,Engström-Laurent A,Lindqvist U

    更新日期:2016-09-01 00:00:00

  • Orthotic management for children with osteogenesis imperfecta.

    abstract::Fitting children and infants who have osteogenesis imperfecta (OI) with braces has posed substantial problems of implementation and patient management For the past twelve years bracing has been an important component of a patient research and management program conducted at the National Institutes of Health. By using ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509116832

    authors: Weintrob JC

    更新日期:1995-01-01 00:00:00

  • Changes in vascular extracellular matrix accumulation reflect phenotypic differences between the arterial wall of pigeons resistant and susceptible to the development of spontaneous atherosclerosis.

    abstract::White Carneau pigeons have previously been shown to be genetically susceptible to the development of spontaneous atherogenesis. The severity of development of atheromatous lesions is considerably greater than a more resistant breed of Show Racer pigeons. Analysis of levels of total hydroxyproline and isodesmosine in t...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209009009813

    authors: Park HS,Kniep AC,Smith SC,Robie SM,Smith EC,Yu SY,Mackenzie JW,Scott GE,Boyd CD

    更新日期:1990-01-01 00:00:00

  • Generation of Hall voltage in bone.

    abstract::Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152629

    authors: Behari J,Andrabi WH

    更新日期:1978-01-01 00:00:00

  • Collagen and proteoglycan production by bovine fetal and adult chondrocytes under low levels of calcium and zinc ions.

    abstract::The experiments described herein tested the effects of CaCl2 and ZnCl2, added at various concentrations in the culture medium, upon the synthesis of collagen and proteoglycan by adult and fetal (articular, epiphyseal and hypertrophic) bovine chondrocytes maintained in high density multilayer cultures. CaCl2 concentrat...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209609000700

    authors: Koyano Y,Hejna M,Flechtenmacher J,Schmid TM,Thonar EJ,Mollenhauer J

    更新日期:1996-01-01 00:00:00

  • Dental structural diseases mapping to human chromosome 4q21.

    abstract::Genetic diseases affecting tooth structure have been classified by the tissue affected enamel versus dentin, and their pattern of inheritance autosomal dominant, autosomal recessive, or X-linked. Advances in molecular genetics and the Human Genome Project have provided substantial progress regarding the identification...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:

    authors: MacDougall M

    更新日期:2003-01-01 00:00:00