Abstract:
:Bovine articular chondrocytes cultured in agarose gel comprise a heterogeneous population when judged by morphological and histochemical criteria. The purpose of the present experiments was to compare, under the same conditions of culture, sub-populations of chondrocytes derived from different depths of articular cartilage. Sub-populations of chondrocytes were cultured separately following their isolation from slices of articular cartilage cut from successive depths of the tissue. Chondrocytes derived from superficial and deep zones differed significantly in morphology, rate of proliferation, and activity in secreting a proteoglycan-rich extracellular matrix. The differences are sufficient to account for the heterogeneity observed in cultures of the entire cell population, and the correlate well with known variations with depth in morphology and histochemistry of intact articular cartilage. These results demonstrate that articular chondrocytes continue in culture to express metabolic differences which reflect their original anatomical location; such differences may have important functional significance.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Aydelotte MB,Kuettner KEdoi
10.3109/03008208809016808subject
Has Abstractpub_date
1988-01-01 00:00:00pages
205-22issue
3eissn
0300-8207issn
1607-8438journal_volume
18pub_type
杂志文章abstract:OBJECTIVE:This study proposes to evaluate, by means of microtomographic analysis, the topical potential of raloxifene combined with BioGran® through the sonochemical method in the repair of critical bone defects in the calvaria of rats. The hypothesis was that the homogenization of Raloxifene to Biogran at the 20% conc...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1430143
更新日期:2018-12-01 00:00:00
abstract:PURPOSE:Orthodontic tooth movement occurs during the bone remodeling induced by therapeutic mechanical strain. It is important to investigate the relation between the strength of mechanical stress and bone formation activity. The aim of this study was to determine the effect of high-magnitude mechanical strain on bone ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2015.1040878
更新日期:2015-01-01 00:00:00
abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Growth factors were historically defined as molecules produced by the body to regulate cell growth and proliferation. After the identification of their receptors and the intracellular signaling machinery they activate, it is now clear that they are involved in the regulation of multiple processes essential for develop...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200802148629
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1485666
更新日期:2018-09-01 00:00:00
abstract::Growth and differentiation factor-5 (GDF-5) is known to induce tendon tissue and stimulate tendon healing. The hypothesis was that adenoviral GDF-5 transfer leads to transitory transgene expression and improves Achilles tendon healing. In vitro experiments were first performed with rat tenocytes. Transgene expression ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200500237120
更新日期:2005-01-01 00:00:00
abstract::A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152380
更新日期:1981-01-01 00:00:00
abstract::Bone and dentin contain many phosphoproteins in their extracellular matrix. It has been postulated that the anionic character of these proteins, and the presence of phosphate groups in particular, is important for various functions related to biomineralization. Phosphophoryns (PP), are the most highly phosphorylated d...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029194
更新日期:1996-01-01 00:00:00
abstract::The paper reviews the changes in ameloblast ultrastructure, concomitant with the changes in its functions across the major stages of amelogenesis. It describes the mechanisms associated with the major events in biosynthesis and degradation of the major enamel proteins (amelogenins and tuftelin/enamelins) and with the ...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509013710
更新日期:1995-01-01 00:00:00
abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1839436
更新日期:2020-12-11 00:00:00
abstract::The fibrous collagens form the structural basis of all mammalian connective tissues, including the vasculature, dermis, bones, tendons, cartilage, and those tissues that support organs such as the heart, kidneys, liver, and lungs. The helical structure of collagen has been extensively studied but in addition to its he...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2010.511353
更新日期:2011-02-01 00:00:00
abstract::The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802171159
更新日期:2008-01-01 00:00:00
abstract::Collagenolytic enzymes were quantitated by a method based on spectrophotometry of suspended reconstituted collagen fibrils. To obtain optically stable suspensions it was necessary to perform a short sonication of the aggregated fibrils at 10 degrees C. When fibrils were cleaved with mammalian fibroblast collagenase at...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152442
更新日期:1991-01-01 00:00:00
abstract::Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collage...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200601143508
更新日期:2007-01-01 00:00:00
abstract::The meniscal cartilages in the knee function to improve congruity of the medial and lateral femoro-tibial joints and play critical roles in load distribution and joint stability. Meniscal tears of various configurations are one of the most common conditions of the knee and are associated with an increased risk of deve...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2017.1284824
更新日期:2017-01-01 00:00:00
abstract::Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::For many years we have investigated the earliest crystal formations of different developing hard tissues (matrix vesicle, bone, dentine, enamel, etc.) by different electron microscopic measurements. It was observed that primarily Ca-phosphate (apatite) "chains," composed of nanometer sized particles (dots, islands), e...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509016998
更新日期:1995-01-01 00:00:00
abstract::Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.775258
更新日期:2013-01-01 00:00:00
abstract::Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.882331
更新日期:2014-06-01 00:00:00
abstract::Fitting children and infants who have osteogenesis imperfecta (OI) with braces has posed substantial problems of implementation and patient management For the past twelve years bracing has been an important component of a patient research and management program conducted at the National Institutes of Health. By using ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509116832
更新日期:1995-01-01 00:00:00
abstract::TGF beta modulates the growth and differentiation of various cell types, in part by regulating the production of extracellular matrix proteins. In rat osteoblast-like cells TGF beta stimulates the production of collagen, osteopontin and osteonectin. On the other hand, TGF beta inhibits the production of osteocalcin, o...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008208909049997
更新日期:1989-01-01 00:00:00
abstract:UNLABELLED:PURPOSE/AIMS OF THE STUDY: Bone's hierarchical structure can be visualized using a variety of methods. Many techniques, such as light and electron microscopy generate two-dimensional (2D) images, while micro-computed tomography (µCT) allows a direct representation of the three-dimensional (3D) structure. In ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2015.1005210
更新日期:2015-04-01 00:00:00
abstract::Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013716
更新日期:1995-01-01 00:00:00
abstract::Size measurements of dispersed rat bone crystals show that with age a greater proportion exceeds 400 A in length. The surface fractures of more heavily mineralized bones tend to be smooth and stepped, whereas those of less mineralized bones are fibrous. These observations combined with information reported elsewhere o...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909050015
更新日期:1989-01-01 00:00:00
abstract::The menisci are intricately organized structures that perform many tasks in the knee. We review their structure and function and introduce new data about their tibial and femoral surfaces. As the femur and tibia approach each other when the knee is bearing load, circumferential tension develops in the menisci, enablin...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008207.2017.1303489
更新日期:2017-01-01 00:00:00
abstract::The use of electron microscopic observations to characterize the state of aggregation of collagen molecules in solution has been examined from the point of view that the observed structures must be compatible with the physical chemical properties of the solution. The common procedure of addition of droplets of a colla...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152630
更新日期:1978-01-01 00:00:00
abstract::To extend previous studies on the apparent acceleration of collagen aging in diabetes mellitus, dura collagen from human adults of different ages, with and without diabetes, was processed to yield soluble and insoluble fractions. Insoluble fractions were cleaved by cyanogen bromide. Release of peptides from insoluble ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309004852
更新日期:1983-01-01 00:00:00
abstract::The diameter of collagen fibrils has been measured in electron micrographs of the tunica media and adventitia of the common carotid artery of 14 rats (19,262 counts). The fibrils of the media were measured in each of its three interlamellar spaces. The mean fibril diameters in the innermost space and the middle space ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709002000
更新日期:1987-01-01 00:00:00
abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909029111
更新日期:1999-01-01 00:00:00
abstract::In the early mineralized enamel crystals, ribbon-like crystals appear near the ameloblasts. Some ribbon-like crystals showed helical or spiral structure within restricted environment during the preparation of embryonic bovine specimens for electron microscope. These specimens did not suffer from the cutting damages no...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017027
更新日期:1998-01-01 00:00:00