Adenovirus-mediated gene transfer of growth and differentiation factor-5 into tenocytes and the healing rat Achilles tendon.

abstract：:Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenovirus...

journal_title：Connective tissue research

authors： Jin QM,Zhao M,Economides AN,Somerman MJ,Giannobile WV

更新日期：2004-01-01 00:00:00

abstract：:To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...

journal_title：Connective tissue research

authors： Tanimoto K,Ohno S,Fujimoto K,Honda K,Ijuin C,Tanaka N,Doi T,Nakahara M,Tanne K

更新日期：2001-01-01 00:00:00

abstract：:Collagen type I fiber-based scaffolds for anterior cruciate ligament (ACL) replacement were evaluated for their mechanical properties and their ability to promote cellular proliferation. Prior to scaffold formation, two crosslinking methods were investigated on individual reconstituted collagen type I fibers, ultravio...

journal_title：Connective tissue research

authors： Walters VI,Kwansa AL,Freeman JW

更新日期：2012-01-01 00:00:00

abstract：:Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...

journal_title：Connective tissue research

authors： Andrades JA,Han B,Nimni ME,Ertl DC,Simpkins RJ,Arrabal MP,Becerra J

更新日期：2003-01-01 00:00:00

abstract：:Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...

journal_title：Connective tissue research

authors： Yao S,Wise G

更新日期：2004-01-01 00:00:00

abstract：:Raman spectra have been determined for hyaluronan, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan monomers and aggregates. The nature of the saccharides and the pattern of sulfation can be discerned. There were only small spectral changes with pH and ionic composition. Differences between hydroxyl vibrations, ...

journal_title：Connective tissue research

authors： Ellis R,Green E,Winlove CP

更新日期：2009-01-01 00:00:00

abstract：:To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenitors. Dental follicle tissue was dissected from the root surface of bovine tooth germ and cells were released by digestion with bacterial collagenase. The released ...

journal_title：Connective tissue research

authors： Handa K,Saito M,Tsunoda A,Yamauchi M,Hattori S,Sato S,Toyoda M,Teranaka T,Narayanan AS

更新日期：2002-01-01 00:00:00

abstract：:A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically...

journal_title：Connective tissue research

authors： Kuboki Y,Saito T,Murata M,Takita H,Mizuno M,Inoue M,Nagai N,Poole AR

更新日期：1995-01-01 00:00:00

abstract：: Purpose/Aim of the study mice are the most often used pre-clinical lab models for studying the pathologies of bone mineralization. However, recent evidence suggests that two of the most often used mice strains (C57BL/6J and CD-1) might show differences in the bone minerali...

journal_title：Connective tissue research

authors： Fayolle C,Labrune M,Berteau JP

更新日期：2020-09-01 00:00:00

abstract：:Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...

journal_title：Connective tissue research

authors： Matsushita T,Matsui N,Fujioka H,Kubo S,Kuroda R,Kurosaka M,Yoshiya S

更新日期：2004-01-01 00:00:00

abstract：:This study aims to elucidate the feasible geometry of the scaffolds in bone and periodontal tissue engineering. Several biomaterials with different geometries are compared in terms of their patterns of ectopic BMP-induced chondrogenesis and osteogenesis. The materials include a honeycomb-shaped hydroxyapatite (HCHAP) ...

journal_title：Connective tissue research

authors： Kuboki Y,Jin Q,Kikuchi M,Mamood J,Takita H

更新日期：2002-01-01 00:00:00

abstract：:Vascular smooth muscle cells (VSMC)s are characterized by their acute growth inhibition by heparin and heparan sulfates; however, recently the isolation of VSMCs which display greatly diminished sensitivity to the antiproliferative action of heparin have been reported. These heparin resistant (HR) VSMCs have been deri...

journal_title：Connective tissue research

authors： San Antonio JD,Verrecchio A,Pukac LA

更新日期：1998-01-01 00:00:00

abstract：:The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...

journal_title：Connective tissue research

authors： Gantt DG,Rafter JA

更新日期：1998-01-01 00:00:00

abstract：:The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabo...

journal_title：Connective tissue research

authors： Benatti BB,Silvério KG,Casati MZ,Sallum EA,Nociti FH Jr

更新日期：2008-01-01 00:00:00

abstract：:Collagenolytic enzymes were quantitated by a method based on spectrophotometry of suspended reconstituted collagen fibrils. To obtain optically stable suspensions it was necessary to perform a short sonication of the aggregated fibrils at 10 degrees C. When fibrils were cleaved with mammalian fibroblast collagenase at...

journal_title：Connective tissue research

authors： Bleeg HS

更新日期：1991-01-01 00:00:00

abstract：:Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium and mesenchyme, while Shh transcripts are present in dental epithelium only, suggesting that SHH functions as an a...

journal_title：Connective tissue research

authors： Wu C,Shimo T,Liu M,Pacifici M,Koyama E

更新日期：2003-01-01 00:00:00

abstract：:We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...

journal_title：Connective tissue research

authors： Fraser J,Lancaster GA,Scriver CR

更新日期：1983-01-01 00:00:00

abstract：:To extend previous studies on the apparent acceleration of collagen aging in diabetes mellitus, dura collagen from human adults of different ages, with and without diabetes, was processed to yield soluble and insoluble fractions. Insoluble fractions were cleaved by cyanogen bromide. Release of peptides from insoluble ...

journal_title：Connective tissue research

authors： Kohn RR

更新日期：1983-01-01 00:00:00

abstract：:We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody...

journal_title：Connective tissue research

authors： Porras-Reyes BH,Blair HC,Jeffrey JJ,Mustoe TA

更新日期：1991-01-01 00:00:00

abstract：:Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture ...

journal_title：Connective tissue research

authors： Pedrozo HA,Schwartz Z,Dean DD,Wiederhold ML,Boyan BD

更新日期：1996-01-01 00:00:00

abstract：:Calbindin-D9k and calbindin-D-28k genes are useful systems to investigate the tissue- and stage-specificity as well as the hormonal control of gene expression. Since they regulate cellular calcium mobilization, their study may be of interest in mineralized tissues. However, thus far, immunocytochemical labelling has b...

journal_title：Connective tissue research

authors： Hotton D,Davideau JL,Bernaudin JF,Berdal A

更新日期：1995-01-01 00:00:00

abstract：:Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...

journal_title：Connective tissue research

authors： Boyd CD,Kniep AC,Pierce RA,Deak SB,Karboski C,Miller DC,Parker MI,Mackenzie JW,Rosenbloom J,Scott GE

更新日期：1988-01-01 00:00:00

abstract：:The composition of glycosaminoglycans (GAGs) was analyzed in skin samples of eight patients suffering from localized scleroderma, i.e., three having generalized morphoea and five localized morphoea plaques. From each patients, biopsies were obtained from sclerotic and perilesional areas, and from clinically uninvolved...

journal_title：Connective tissue research

authors： Møller R,Serup J,Ammitzbøll T

更新日期：1985-01-01 00:00:00

abstract：:The novel method of high voltage electron microscopic tomography (3D) has been applied for the first time to examine ultrastructural features and spatial relations between collagen fibrils and mineral crystals in a mouse mutant (oim/oim) which replicates a moderate to severe form of osteogenesis imperfecta. The animal...

journal_title：Connective tissue research

authors： Landis WJ

更新日期：1995-01-01 00:00:00

abstract：:Degeneration of the intervertebral discs, a natural progression of the aging process, is strongly implicated as a cause of low back pain. Aggrecan is the major structural proteoglycan in the extracellular matrix of the intervertebral disc. It is large, possessing numerous glycosaminoglycan chains and the ability to fo...

journal_title：Connective tissue research

authors： Wei Q,Zhang X,Zhou C,Ren Q,Zhang Y

更新日期：2019-05-01 00:00:00

abstract：:An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pe...

journal_title：Connective tissue research

authors： Arias JL,Fernandez MS,Dennis JE,Caplan AI

更新日期：1991-01-01 00:00:00

abstract：:The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...

journal_title：Connective tissue research

authors： Smith CE,Chen WY

更新日期：1998-01-01 00:00:00

abstract：:To study the glycosylation of glomerular basement membrane collagen (GBMC) in diabetes, kidneys were obtained at autopsy from 5 patients with insulin-requiring diabetes of long duration and diabetic complications, and from 5 control subjects. Glomeruli were prepared by sieving and collagen was isolated by limited peps...

journal_title：Connective tissue research

authors： Uitto J,Perejda AJ,Grant GA,Rowold EA,Kilo C,Williamson JR

更新日期：1982-01-01 00:00:00

abstract：:To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...

journal_title：Connective tissue research

authors： Mehta SS,Antich PP,Landis WJ

更新日期：1999-01-01 00:00:00

abstract：:Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...

journal_title：Connective tissue research

authors： Giraud-Guille MM,Besseau L

更新日期：1998-01-01 00:00:00