Roles of large aggregating proteoglycans in human intervertebral disc degeneration.

Abstract:

:Degeneration of the intervertebral discs, a natural progression of the aging process, is strongly implicated as a cause of low back pain. Aggrecan is the major structural proteoglycan in the extracellular matrix of the intervertebral disc. It is large, possessing numerous glycosaminoglycan chains and the ability to form aggregates in association with hyaluronan. The negatively charged glycosaminoglycan side chains in aggrecan in the nucleus pulposus of the intervertebral discs can bind electrostatically to polar water molecules, which are crucial for maintaining the well-hydrated state that enables the discs to undergo reversible deformation under compressive loading. A more in-depth understanding of the molecular basis of disc degeneration is essential to the design of therapeutic solutions to treat degenerative discs. Within this scope, we discuss the current knowledge concerning the structure and function of aggrecan in intervertebral disc degeneration. These data suggest that aggrecan plays a central role in the function and degeneration of the intervertebral disc, which may suggest potential aggrecan-based therapies for disc regeneration.

journal_name

Connect Tissue Res

authors

Wei Q,Zhang X,Zhou C,Ren Q,Zhang Y

doi

10.1080/03008207.2018.1499731

subject

Has Abstract

pub_date

2019-05-01 00:00:00

pages

209-218

issue

3

eissn

0300-8207

issn

1607-8438

journal_volume

60

pub_type

杂志文章,评审
  • An address to young research workers: Inconsequences and blindness to the facts and unpublished observations.

    abstract::The critical remembrance of my research activity displays aspects of inconsequence and mental rigidity. Particular probative examples are called to mind. Unpublished observations concerning immortalized dental cells are summarized. As a consequence, if I could start again, I would improve several aspects of my behavio...

    journal_title:Connective tissue research

    pub_type:

    doi:10.1080/03008200290001195

    authors: Ruch JV

    更新日期:2002-01-01 00:00:00

  • Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1.

    abstract::The efficacy of connective tissue explants is difficult to determine, particularly where autopsy material is required for research or clinical applications. We report here an optimised protocol using 5-chloromethylfluorescein diacetate (CMFDA) and ethidium homodimer-1 to distinguish viable and non-viable cells in a ra...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209609028880

    authors: Poole CA,Brookes NH,Gilbert RT,Beaumont BW,Crowther A,Scott L,Merrilees MJ

    更新日期:1996-01-01 00:00:00

  • Effect of temperature on the metabolism of proteoglycans in explants of bovine articular cartilage.

    abstract::The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both t...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109152166

    authors: Brand HS,De Koning MH,van Kampen GP,Van der Korst JK

    更新日期:1991-01-01 00:00:00

  • In vivo expression of classic PKC isoforms in the rat dental follicle as related to tooth eruption.

    abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008200490882628

    authors: Yao S,Wise G

    更新日期:2004-01-01 00:00:00

  • Increased elastin mRNA levels associated with surgically induced intimal injury.

    abstract::Quantitative levels of mRNAs coding for elastin, types I and III procollagen and gamma-actin were measured in porcine vascular material following balloon catheterization. A balloon catheter was introduced into the thoracic aorta and jugular vein of 3-6 week old pigs; following distention and six days of postoperative ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208809008059

    authors: Boyd CD,Kniep AC,Pierce RA,Deak SB,Karboski C,Miller DC,Parker MI,Mackenzie JW,Rosenbloom J,Scott GE

    更新日期:1988-01-01 00:00:00

  • Collagens of the chicken eggshell membranes.

    abstract::An immunohistochemical analysis of the eggshell membranes shows the occurrence of type X collagen while type I collagen was not detected by using an appropriate monoclonal antibody with untreated shell membranes. A positive immuno-reaction for type I collagen was obtained after digestion of the shell membranes with pe...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109152162

    authors: Arias JL,Fernandez MS,Dennis JE,Caplan AI

    更新日期:1991-01-01 00:00:00

  • Generation of Hall voltage in bone.

    abstract::Hall type voltage has been observed in bone and its two major components (collagen and apatite) in the region of moderately high electric and magnetic fields. The configuration of the sample placement and the experimental set up was the same as that adopted in standard Hall measurements. The resistivity of the sample ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207809152629

    authors: Behari J,Andrabi WH

    更新日期:1978-01-01 00:00:00

  • Water content of equine articular cartilage: effects of enzymatic degradation and "artificial fibrillation".

    abstract::A method is described for accurate and reproducible determination of wet and dry weights of small articular cartilage samples. The water content of the articular cartilage samples before and after treatment with selective degradative enzymes and "artificial fibrillation" was determined. It was found that the water con...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208209160266

    authors: Simon WH,Wohl DL

    更新日期:1982-01-01 00:00:00

  • Common gene variants interactions related to uric acid transport are associated with knee osteoarthritis susceptibility.

    abstract:BACKGROUND:The presence of genetic variants in uric acid (UA) transporters can be associated with hyperuricemia, and therefore with an increased risk of monosodium urate (MSU) crystal precipitation. The inflammatory process triggered by these crystals leads to cartilage damage, which, in turn, could promote knee osteoa...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2018.1483359

    authors: Fernández-Torres J,Martínez-Nava GA,Oliviero F,López-Reyes AG,Martínez-Flores K,Garrido-Rodríguez D,Francisco-Balderas A,Zamudio-Cuevas Y

    更新日期:2019-05-01 00:00:00

  • The effects of aging upon the connective tissues of the periodontal ligament.

    abstract::The ultrastructural features of the cells and of the oxytalan fibers within the periodontal ligaments of aged rats (2 year old) were quantified and compared with data for tissues obtained from younger animals (8 week old). Sections of the mid-root regions of the mandibular first molars were prepared for examination by...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509016978

    authors: Moxham BJ,Evans IL

    更新日期:1995-01-01 00:00:00

  • Cross-linking and stereospecific structure of collagen in mineralized and nonmineralized skeletal tissues.

    abstract::Molecular distributions of the intermolecular cross-links in fetal bovine bone type I collagen fibrils were quantitatively determined and compared with those of periodontal ligament. Results indicated that Hyl and Lys residues in the COOH-terminal nonhelical peptide portions (residues 16C) of both alpha 1 chains were ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208909050006

    authors: Yamauchi M,Katz EP,Otsubo K,Teraoka K,Mechanic GL

    更新日期:1989-01-01 00:00:00

  • The character of gene expression of human periosteum used to form new tissue in allograft bone.

    abstract::Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2014.923851

    authors: Kemppainen J,Yu Q,Alexander J,Jacquet R,Scharschmidt T,Landis W

    更新日期:2014-08-01 00:00:00

  • Coordinated expression of MMPs and TIMPs in rat knee intra-articular tissues after ACL injury.

    abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:

    authors: Tang Z,Yang L,Zhang J,Xue R,Wang Y,Chen PC,Sung KL

    更新日期:2009-01-01 00:00:00

  • Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer.

    abstract::The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200109016840

    authors: Gautron J,Hincke MT,Panheleux M,Garcia-Ruiz JM,Boldicke T,Nys Y

    更新日期:2001-01-01 00:00:00

  • Cellular interactions regulate stem cell differentiation in tri-culture.

    abstract::Currently, the mechanism governing the regeneration of the soft tissue-to-bone interface, such as the transition between the anterior cruciate ligament (ACL) and bone, is not known. Focusing on the ACL-to-bone insertion, this study tests the novel hypothesis that interactions between cells from the ligament (fibroblas...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2016.1230106

    authors: Wang IE,Bogdanowicz DR,Mitroo S,Shan J,Kala S,Lu HH

    更新日期:2016-11-01 00:00:00

  • Induced hypothyroidism alters articular cartilage in skeletally immature miniature swine.

    abstract:PURPOSE/AIM:Thyroid hormone has been implicated in the normal growth and development of articular cartilage; however, its effect on a disease state, such as hypothyroidism, is unknown. The purpose of this investigation was to compare normal articular cartilage from proximal femurs of immature miniature swine to proxima...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2020.1839436

    authors: Bundy JA,Yang JT,Morscher MA,Steiner RP,Adamczyk MJ,Weiner DS,Jacquet RD,Landis WJ

    更新日期:2020-12-11 00:00:00

  • Removal of dentin non-collagenous structures results in the unraveling of microfibril bundles in collagen type I.

    abstract:AIMS:The structural organization of collagen from mineralized tissues, such as dentin and bone, has been a topic of debate in the recent literature. Recent reports have presented novel interpretations of the complexity of collagen type I at different hierarchical levels and in different tissues. Here, we investigate th...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.1080/03008207.2016.1235566

    authors: Bertassoni LE,Swain MV

    更新日期:2017-09-01 00:00:00

  • Tomographic imaging of collagen-mineral interaction: implications for osteogenesis imperfecta.

    abstract::The novel method of high voltage electron microscopic tomography (3D) has been applied for the first time to examine ultrastructural features and spatial relations between collagen fibrils and mineral crystals in a mouse mutant (oim/oim) which replicates a moderate to severe form of osteogenesis imperfecta. The animal...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209509010825

    authors: Landis WJ

    更新日期:1995-01-01 00:00:00

  • Coordinate regulation of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expression in human vascular smooth muscle cells.

    abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200009067666

    authors: Kato S,Yasukawa H,Fujii T,Yamaguchi M,Miyagi N,Okamoto K,Wada Y,Miyamoto T,Morimatsu M,Fox JC

    更新日期:2000-01-01 00:00:00

  • Time-dependent properties of human umbilical fascia.

    abstract::This investigation is devoted to the study of the viscoelastic behavior of human abdominal fascia from the umbilical region. Seventeen samples 10 mm wide and up to 70 mm long were cut along the primary fiber direction (group FL) or perpendicular to it (group FT) and subjected to relaxation tests. The viscoelastic resp...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008207.2011.604452

    authors: Kirilova M

    更新日期:2012-01-01 00:00:00

  • Determination of collagen fibril orientation in the cartilage of vertebral end plate.

    abstract::X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208109160244

    authors: Aspden RM,Hickey DS,Hukins DW

    更新日期:1981-01-01 00:00:00

  • Banded patterns in liquid crystalline phases of type I collagen: relationship with crimp morphology in connective tissue architecture.

    abstract::Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209809002438

    authors: Giraud-Guille MM,Besseau L

    更新日期:1998-01-01 00:00:00

  • Interactions of elastin and microfibrils in elastogenesis of human pulmonary fibroblasts in culture.

    abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209309016835

    authors: Fukuda Y,Nakazawa N,Yamanaka N

    更新日期:1993-01-01 00:00:00

  • Helix structure of ribbon-like crystals in bovine enamel.

    abstract::In the early mineralized enamel crystals, ribbon-like crystals appear near the ameloblasts. Some ribbon-like crystals showed helical or spiral structure within restricted environment during the preparation of embryonic bovine specimens for electron microscope. These specimens did not suffer from the cutting damages no...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209809017027

    authors: Suzuki K,Sakae T,Kozawa Y

    更新日期:1998-01-01 00:00:00

  • The role of SLRP-proteoglycans in osteosarcoma pathogenesis.

    abstract::The small leucine-rich repeat proteoglycans (SLRPs) constitute a group of structurally and functionally related molecules that participate in the organization of the extracellular matrix ECM and have important effects on cell behavior. Osteosarcomas are heterogeneous bone tumors whose common characteristic is the prod...

    journal_title:Connective tissue research

    pub_type: 杂志文章,评审

    doi:10.1080/03008200802147589

    authors: Nikitovic D,Berdiaki K,Chalkiadaki G,Karamanos N,Tzanakakis G

    更新日期:2008-01-01 00:00:00

  • Binding of lysyl oxidase to fibrils of type I collagen.

    abstract::The binding of highly purified bovine aortic lysyl oxidase to native fibrils of type I collagen has been measured by assay of unbound lysyl oxidase activity in the supernatants of enzyme-collagen mixtures after centrifugation. The apparent binding affinity of lysyl oxidase for native fibrils is quite similar to that f...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208509015017

    authors: Cronlund AL,Smith BD,Kagan HM

    更新日期:1985-01-01 00:00:00

  • A restriction fragment length polymorphism in the pigeon pro alpha 2(1) collagen gene: lack of an allelic association with an atherogenic phenotype in pigeons genetically susceptible to the development of spontaneous atherosclerosis.

    abstract::A high frequency restriction fragment length polymorphism (RFLP) at the 3'-end of the pigeon pro alpha 2(1) collagen gene was detected using the restriction endonuclease EcoR1. The distribution of this allelic variant was analyzed in DNA isolated from White Carneau pigeons genetically susceptible to the development of...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008209109152437

    authors: Boyd CD,Song JY,Kniep AC,Park HS,Fastnacht C,Smith EC,Smith SC

    更新日期:1991-01-01 00:00:00

  • An estimate of the mean length of collagen fibrils in rat tail-tendon as a function of age.

    abstract::A theoretical expression has been derived for the mean collagen fibril length in tendon based on the assumption that collagen fibrils originate in cell surface invaginations and terminate either at some remote cell surface or another collagen fibril bundle. The expression thus determined requires knowledge of the effe...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208909016814

    authors: Craig AS,Birtles MJ,Conway JF,Parry DA

    更新日期:1989-01-01 00:00:00

  • Ultrastructural organization of skin: classification on the basis of mechanical role.

    abstract::In our previous developmental study on skin we reported that correlations existed between the collagen fibril diameter distribution, glycosaminoglycan content and composition, and mechanical role. In this work we present new but related electron microscope data on the ultrastructure of skins from flounder, marlin, cod...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008208709006977

    authors: Craig AS,Eikenberry EF,Parry DA

    更新日期:1987-01-01 00:00:00

  • Expression analysis of angiogenic growth factors and biological axis CXCL12/CXCR4 axis in idiopathic pulmonary fibrosis.

    abstract::Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endo...

    journal_title:Connective tissue research

    pub_type: 杂志文章

    doi:10.3109/03008200903056150

    authors: Antoniou KM,Soufla G,Lymbouridou R,Economidou F,Lasithiotaki I,Manousakis M,Drositis I,Spandidos DA,Siafakas NM

    更新日期:2010-01-01 00:00:00