Abstract:
:A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically different solid-state carriers induced tissues in quite different manners. FGM/BMP implants induced cartilage formation within the entire inner area of the membrane accompanied by a small amount of bone formation on the surface of the membrane. In contrast, PPHAP/BMP implants induced only bone within the pores of PPHAP without any detectable cartilage formation. Enzyme-linked immunosorbent assay revealed that the type II collagen content in FGM/BMP was six times higher than that in IBM/BMP, while there was no detectable type II collagen in PPHAP/BMP. The results were explained by the geometric properties of the two distinctive carriers.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Kuboki Y,Saito T,Murata M,Takita H,Mizuno M,Inoue M,Nagai N,Poole ARdoi
10.3109/03008209509013726subject
Has Abstractpub_date
1995-01-01 00:00:00pages
219-26issue
1-4eissn
0300-8207issn
1607-8438journal_volume
32pub_type
杂志文章abstract::This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatogr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009152156
更新日期:1990-01-01 00:00:00
abstract::X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109160244
更新日期:1981-01-01 00:00:00
abstract::The relationship between structural changes and hyaluronic acid (HA) content in muscular atrophy is unknown. The objective of our study was to conduct multifaceted evaluations of the change in muscle extensibility in muscular atrophy, in terms of morphological aspects as well as from a biochemical viewpoint, including...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200590954131
更新日期:2005-01-01 00:00:00
abstract::Objective: Aseptic loosening is a major problem in total joint replacement. Implant wear debris provokes a foreign body host response and activates cells to produce a variety of mediators and ROS, leading to periprosthetic osteolysis. Elevated ROS levels can harm proteasome function. Proteasome inhibitors have been re...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1601186
更新日期:2019-11-01 00:00:00
abstract::The structural and immunological properties of the glycosaminoglycans and the core proteins of bovine nasal cartilage proteoglycan and the proteoglycans produced by rabbit articular chondrocytes in spinner and monolayer culture were compared. Culture medium with 35SO4- or 3H-serine-labeled proteoglycan was mixed with ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309004860
更新日期:1983-01-01 00:00:00
abstract::Positive effects on the tensile characteristics of swine digital extensors were found following twelve months of exercise training. Compared to sedentary controls, the tendons from the exercised animals became stronger as a material and exhibited hypertrophy. These biomechanical results were supported by biochemical a...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208009152109
更新日期:1980-01-01 00:00:00
abstract::The biological effects of isolated soluble dentin extracellular matrix components on the induction of reparative dentinogenesis in exposed cavities in ferret canine teeth have been shown to be blocked by immobilizing the extracellular matrix components on nitrocellulose or Millipore membranes during implantation. This...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013736
更新日期:1995-01-01 00:00:00
abstract::We have investigated the changes in some of the biochemical and biophysical properties of human femoral head cartilage on the one hand during ageing and on the other hand in osteoarthritis. Topographical variations were also investigated. The parameters studied were those relevant to cartilage function, viz., proteogl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909043895
更新日期:1989-01-01 00:00:00
abstract::The present study details structural aspects of the cells of the rat intra-articular disc (IAD) of the temporomandibular joint. At the light and electron microscope level, the outline of the cells varied, although a number appeared oval/rounded. Ultrastructurally, the cells contained moderate amounts of the intracellu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Cumulative damage to long-lived connective tissue proteins play a key role in the development of age-related human diseases such as cardiovascular stiffening and age-related macular degeneration. The processes that result in the accumulation of increasingly insoluble, undigestible damaged collagen are only partially k...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600721569
更新日期:2006-01-01 00:00:00
abstract::The composition of glycosaminoglycans (GAGs) was analyzed in skin samples of eight patients suffering from localized scleroderma, i.e., three having generalized morphoea and five localized morphoea plaques. From each patients, biopsies were obtained from sclerotic and perilesional areas, and from clinically uninvolved...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509152402
更新日期:1985-01-01 00:00:00
abstract::The oxidation of an elastin substrate by highly purified bovine aortic lysyl oxidase (LO) is markedly influenced by amphiphilic molecules known to bind to elastin. Negatively charged elastin ligands, including fatty acids, bile salts or sodium dodecyl sulfate can completely inhibit the oxidation of lysine in elastin, ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152378
更新日期:1981-01-01 00:00:00
abstract::We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309015011
更新日期:1983-01-01 00:00:00
abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923865
更新日期:2014-08-01 00:00:00
abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152217
更新日期:1976-01-01 00:00:00
abstract::Ameloblastic tissue samples from unerupted bone molars were used to prepare subcellular enamel protein kinase preparations, nuclear + plasma membrane, cytosolic and microsomal, and used in in vitro phosphorylation of purified 20 kDa bovine amelogenin in the presence of 32P-ATP. Both cytosolic and microsomal preparatio...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809017041
更新日期:1998-01-01 00:00:00
abstract::A theoretical expression has been derived for the mean collagen fibril length in tendon based on the assumption that collagen fibrils originate in cell surface invaginations and terminate either at some remote cell surface or another collagen fibril bundle. The expression thus determined requires knowledge of the effe...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909016814
更新日期:1989-01-01 00:00:00
abstract::Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200903056150
更新日期:2010-01-01 00:00:00
abstract::In our previous developmental study on skin we reported that correlations existed between the collagen fibril diameter distribution, glycosaminoglycan content and composition, and mechanical role. In this work we present new but related electron microscope data on the ultrastructure of skins from flounder, marlin, cod...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709006977
更新日期:1987-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are members of the Metzincin family of proteases responsible for degrading the extracellular matrix (ECM). In early studies, MMP degradation of the sub-epithelial basement membrane was thought to be tumor cell autonomous and contribute to the invasive behavior of malignant cells. It is...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2013.867339
更新日期:2014-01-01 00:00:00
abstract::Several studies have highlighted the lack of age changes in the extracellular matrix of the periodontal ligament, but more needs to be known about cellular and functional changes (including the effects upon eruption). For this study, impeded and unimpeded eruption rates were measured over a 2-week period for the mandi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000754
更新日期:2002-01-01 00:00:00
abstract::Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802143141
更新日期:2008-01-01 00:00:00
abstract::Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207709152610
更新日期:1977-01-01 00:00:00
abstract::Proteoglycans extracted from articular cartilage of large joints of humans aged 4, 11, 70 and 75, were fractionated on associative density gradients. The top fraction (A3) was purified by ion-exchange chromatography and subsequent gel filtration on Sepharose CL 4B in 4 M GuCl, 0.5% Triton x 100. Proteoglycans from you...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809017475
更新日期:1988-01-01 00:00:00
abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490882628
更新日期:2004-01-01 00:00:00
abstract::The matrix metalloproteinases collagenase (MMP-1) and stromelysin (MMP-3) are often coordinately expressed, and their promoters contain similar regulatory elements, including an AP-1 site at about -70. There are, however, additional sequences including an adjacent ETS site at about -90 in both promoters, and a NIP (nu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160231
更新日期:1997-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabo...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200802171159
更新日期:2008-01-01 00:00:00
abstract:PURPOSE/AIM:Glutamate is one of the signaling molecules responsible for transmission in the central nervous system. Periodontal ligament (PDL) cells were recently reported to express metabotropic glutamate receptors (mGluRs). However, the functions of mGluR signaling in PDL cells or PDL-related cells remain largely unk...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2016.1195826
更新日期:2016-09-01 00:00:00
abstract::STR/ort mice develop a naturally occurring osteoarthritis (OA) of the knee joints. However, the evaluation of early OA changes has been difficult due to variability caused by gender, individual differences, and differences between the right and left lower limbs. The objective of this study was to analyze the variabili...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200902836065
更新日期:2009-01-01 00:00:00