Abstract:
:The matrix metalloproteinases collagenase (MMP-1) and stromelysin (MMP-3) are often coordinately expressed, and their promoters contain similar regulatory elements, including an AP-1 site at about -70. There are, however, additional sequences including an adjacent ETS site at about -90 in both promoters, and a NIP (nuclear inhibitory protein) binding site in the stromelysin promoter. In this paper, we have investigated the role of these elements in transcriptional activation by phorbol myristate acetate (PMA). Using mobility shift assays, we demonstrate that in the collagenase promoter, PMA induction requires the binding of nuclear proteins to the ETS site as well as to the adjacent AP-1 element. In the stromelysin promoter, we used mutational analysis and DNA/protein interactions to illustrate a role for a single ETS site and for the NIP element in phorbol induction. These data suggest that ETS elements interact with other cis-acting sequences in these promoters to elicit transcriptional activation, and that the placement of the ETS sites in these promoters may influence transcriptional activity.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
White LA,Maute C,Brinckerhoff CEdoi
10.3109/03008209709160231subject
Has Abstractpub_date
1997-01-01 00:00:00pages
321-35issue
4eissn
0300-8207issn
1607-8438journal_volume
36pub_type
杂志文章abstract::Syndecan-4 is emerging as an important player in cell interaction with the extracellular environment and has been shown to be involved in the progression of intervertebral disc degeneration. However, the mechanism of syndecan-4 regulation by TNF-α and the role of TGF-β1 in regulating syndecan-4 expression remain poorl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.996702
更新日期:2015-01-01 00:00:00
abstract::Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1702031
更新日期:2020-05-01 00:00:00
abstract::Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809002438
更新日期:1998-01-01 00:00:00
abstract:UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923875
更新日期:2014-08-01 00:00:00
abstract::To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109005649
更新日期:2001-01-01 00:00:00
abstract::Two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either HaeIII/AluI or Sau3A and contained in lambda Charon 4A or EMBL3, respectively. The 6 kb of DNA comprising the most 3' portion of the gene were sequenced demonstrati...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709006976
更新日期:1987-01-01 00:00:00
abstract::Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth pl...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1394295
更新日期:2018-01-01 00:00:00
abstract::Aggrecan is the prominent proteoglycan in cartilage and is modified with approximately 100 chondroitin sulfate (CS) chains through a tetrasaccharide linkage structure. In osteoarthritis (OA), the viscoelastic properties of cartilage are compromised on both the quantity and integrity of aggrecan core protein expressed ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2012.756871
更新日期:2013-01-01 00:00:00
abstract::Purpose: Non-pathological child cortical bone (NPCCB) studies can provide clinicians with vital information and insights. However, assessing the anisotropic elastic properties of NPCCB remains a challenge for the biomechanical engineering community. For the first time, this paper provides elastic moduli values for NPC...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1570170
更新日期:2019-07-01 00:00:00
abstract::Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152287
更新日期:1978-01-01 00:00:00
abstract::Odontogenesis involves a complex series of processes including epithelial-mesenchymal interactions, morphogenesis, differentiation, fibrillogenesis, and mineralization. Extracellular (ECM) remodeling plays a critical role in the rapid morphological changes that accompany these events. It is proposed that matrix metall...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000538
更新日期:2002-01-01 00:00:00
abstract::Volume and morphology of chondrocytes in osteoarthritic human hip joint articular cartilage were characterized, and their relationship to tissue structure and function was determined. Human osteochondral articular cartilage samples (n=16) were obtained from the femoral heads of nine patients undergoing total hip arthr...
journal_title:Connective tissue research
pub_type: 临床试验,杂志文章
doi:10.3109/03008207.2014.912645
更新日期:2014-08-01 00:00:00
abstract::Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.546536
更新日期:2011-10-01 00:00:00
abstract::We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309015011
更新日期:1983-01-01 00:00:00
abstract::We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Fu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909029111
更新日期:1999-01-01 00:00:00
abstract:OVERVIEW:Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance; however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of PO...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1306060
更新日期:2018-03-01 00:00:00
abstract::The expression of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) by human vascular smooth muscle cells (SMC) was monitored as a function of the phenotypic modulation in vitro. Cell phenotype was manipulated by varying serum concentration and cell density. Synthetic phenotype was characterized by a minim...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200009067666
更新日期:2000-01-01 00:00:00
abstract::DNA synthesis in rat and rabbit polyvinyl sponge induced granulation tissue has been studied using thymidine (methyl-3H). Synthesis was determined by measurement of thymidine incorporation into cold trichloroacetic acid insoluble material and by autoradiography. Granulation tissue was removed and immediately incubated...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152225
更新日期:1976-01-01 00:00:00
abstract::The composition of glycosaminoglycans (GAGs) was analyzed in skin samples of eight patients suffering from localized scleroderma, i.e., three having generalized morphoea and five localized morphoea plaques. From each patients, biopsies were obtained from sclerotic and perilesional areas, and from clinically uninvolved...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208509152402
更新日期:1985-01-01 00:00:00
abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923865
更新日期:2014-08-01 00:00:00
abstract::Rat bone marrow cells were cultured in vitro in a collagen-gel medium at 0.5% fetal bovine serum concentration for 10 days in the presence of recombinant human transforming growth factor-beta-1, genetically engineered to contain a collagen binding domain (rhTGF-beta1-F2), or a commercial rhTGF-beta1. To compare the ef...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200390229912
更新日期:2003-01-01 00:00:00
abstract::An extensive literature on bone morphogenetic protein (BMP) induced generation and regeneration shows general agreement about one observation. The incidence and quantity of bone were greatest when BMP was delivered with a carrier of various biologic and non biologic polymers. In the present research, neutral lipids ei...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160210
更新日期:1997-01-01 00:00:00
abstract::The mechanical properties of collagen as a biopolymer ensures that collagen has a significant influence on the mechanical behavior of the host tissue. Structural organization is a key to that influence. We have assessed this relationship quantitatively in the tunica media of arteries from the heart and brain, using th...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152168
更新日期:1991-01-01 00:00:00
abstract::Periosteal explants obtained from rabbit calvariae are introduced as a model system to study contraction of soft connective tissue. Culturing of these explants resulted in a substantial time-dependent decrease of the surface area which coincided with a similar decrease in volume. A 75% reduction of the initial explant...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509028402
更新日期:1995-01-01 00:00:00
abstract::Stimulation of the cholinergic inflammatory pathway can attenuate collagen-induced arthritis (CIA) and inhibit synovitis by Janus kinase (JAK) 2 and signal transducer and activator of transcription (STAT) 3 signaling. Suppressor of cytokine signaling (SOCS) protein can also regulate the inflammatory processes and acti...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1380633
更新日期:2018-05-01 00:00:00
abstract::The eggshell is an highly ordered structure deposited in the distal oviduct and composed of calcium carbonate and an organic matrix which is believed to influence its fabric. We have identified ovotransferrin as an 80 kDa matrix protein observed at high concentration in the uterine fluid at the initial stage of shell ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200109016840
更新日期:2001-01-01 00:00:00
abstract::Purpose/Aim: Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from vascular smooth muscle cells significantly contribute to the initiation and progression of this pathology. Recently, we have demonstrated that elevated p...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1444759
更新日期:2018-12-01 00:00:00
abstract::Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular pro...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209009152421
更新日期:1990-01-01 00:00:00
abstract::The purpose of this study was to investigate overall degradative changes occurring to enamel matrix proteins in small, freeze-dried pieces of rat incisor enamel homogenized and incubated directly for 0-48 hours in a synthetic enamel fluid solution (165 mM total ionic strength with 0.153 mM calcium chloride) versus oth...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023914
更新日期:1998-01-01 00:00:00
abstract::The mechanical properties of the human supraspinatus tendon (SST) are highly heterogeneous and may reflect an important adaptive response to its complex, multiaxial loading environment. However, these functional properties are associated with a location-dependent structure and composition that have not been fully eluc...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.847096
更新日期:2013-01-01 00:00:00