Proteoglycans synthesized by fetal guinea pig chondrocytes in culture.

abstract：:Solutions of type I acid soluble collagen were studied in light and electron microscopy at concentrations over 40 mg/ml. Banded patterns spontaneously emerge in samples observed between crossed polars between slide and coverslip. The textures are interpreted as precholesteric, appearing at the transition between the i...

journal_title：Connective tissue research

authors： Giraud-Guille MM,Besseau L

更新日期：1998-01-01 00:00:00

abstract：:Scanning electron microscopy and high angle X-ray diffraction were used to define the relationship between collagen and elastin of bovine aortic wall. The diffraction pattern shows on one hand that the broad rings at 4.5 A and 9 A, due to elastin, do not orient on stretching and on the other hand, that the collagen ri...

journal_title：Connective tissue research

authors： Bigi A,Ripamonti A,Roveri N

更新日期：1977-01-01 00:00:00

abstract：:Many organisms from a wide variety of taxa produce amorphous calcium carbonate (ACC), despite the fact that it is inherently unstable and relatively soluble in its pure state. These properties also make it difficult to detect and characterize ACC. Raman spectroscopy is a particularly useful method for investigating AC...

journal_title：Connective tissue research

authors： Weiner S,Levi-Kalisman Y,Raz S,Addadi L

更新日期：2003-01-01 00:00:00

abstract：:Currently, the mechanism governing the regeneration of the soft tissue-to-bone interface, such as the transition between the anterior cruciate ligament (ACL) and bone, is not known. Focusing on the ACL-to-bone insertion, this study tests the novel hypothesis that interactions between cells from the ligament (fibroblas...

journal_title：Connective tissue research

authors： Wang IE,Bogdanowicz DR,Mitroo S,Shan J,Kala S,Lu HH

更新日期：2016-11-01 00:00:00

abstract：:Purpose: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition...

journal_title：Connective tissue research

authors： Matheson A,Regmi SC,Martin-Alarcon L,Jay GD,Scott WM,Schmidt TA

更新日期：2020-04-20 00:00:00

abstract：:Ligaments have limited regenerative potential and as a consequence, repair is protracted and results in a mechanically inferior tissue more scar-like than native ligament. We previously reported that a single injection of interleukin-1 receptor antagonist (IL-1Ra) delivered at the time of injury, decreased the number ...

journal_title：Connective tissue research

authors： Chamberlain CS,Leiferman EM,Frisch KE,Duenwald-Kuehl SE,Brickson SL,Murphy WL,Baer GS,Vanderby R

更新日期：2014-06-01 00:00:00

abstract：:Monoclonal antibodies (mAbs) (4F2 and 7E12) were prepared against heparan sulfate proteoglycan (HSPG) isolated from bovine glomeruli. Enzyme linked immunosorbent assays (ELISA) and immunoblotting demonstrated that the mABs reacted with HSPG. Indirect immunofluorescence (IF) showed that the mAbs stained renal basement ...

journal_title：Connective tissue research

authors： Kemeny E,Fillit HM,Damle S,Mahabir R,Kefalides NA,Gregory JD,Antonovych T,Sabnis S,Zabriskie JB

更新日期：1988-01-01 00:00:00

abstract：:For many years we have investigated the earliest crystal formations of different developing hard tissues (matrix vesicle, bone, dentine, enamel, etc.) by different electron microscopic measurements. It was observed that primarily Ca-phosphate (apatite) "chains," composed of nanometer sized particles (dots, islands), e...

journal_title：Connective tissue research

authors： Höhling HJ,Arnold S,Barckhaus RH,Plate U,Wiesmann HP

更新日期：1995-01-01 00:00:00

abstract：:This study evaluated the effect of short bouts of stretching on the soleus muscle after immobilization, by measuring the birefringence of the intramuscular connective tissue (ICT) and the muscle fiber area. Thirty rats were divided into five groups: the left soleus was immobilized in the shortened position; after immo...

journal_title：Connective tissue research

authors： Coutinho EL,DeLuca C,Salvini TF,Vidal BC

更新日期：2006-01-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenovirus...

journal_title：Connective tissue research

authors： Jin QM,Zhao M,Economides AN,Somerman MJ,Giannobile WV

更新日期：2004-01-01 00:00:00

abstract：:The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...

journal_title：Connective tissue research

authors： Hall DA

更新日期：1976-01-01 00:00:00

abstract：:In orthodontic tooth movement, prostaglandin E(2) (PGE(2)) released from osteoblasts can alter the normal process of bone remodeling. We examined the effect of compressive force (CF) on PGE(2) production, PGE receptors (Ep1-4) expression, phosphorylation of protein kinase A (p-PKA), and calcium content in Saos-2 cells...

journal_title：Connective tissue research

authors： Sanuki R,Mitsui N,Suzuki N,Koyama Y,Yamaguchi A,Isokawa K,Shimizu N,Maeno M

更新日期：2007-01-01 00:00:00

abstract：:Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...

journal_title：Connective tissue research

authors： Register TC,Wagner WD

更新日期：1990-01-01 00:00:00

abstract：:Human skin fibroblasts cultured with 4-methylumbelliferone (MU), a hyaluronan synthesis inhibitor, produce a hyaluronan-deficient extracellular matrix (See [9]). Our present study investigated the effects of MU on proteoglycan, which is the other main component of the extracellular matrix, and interacts with hyalurona...

journal_title：Connective tissue research

authors： Funahashi M,Nakamura T,Kakizaki I,Mizunuma H,Endo M

更新日期：2009-01-01 00:00:00

abstract：:To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry ...

journal_title：Connective tissue research

authors： Mehta SS,Antich PP,Landis WJ

更新日期：1999-01-01 00:00:00

abstract：:Elastic ear cartilage and aortae from nine-day-old hamsters were incubated in Krebs-Ringer medium containing ascorbate, beta-aminopropionitrile and either [14C]proline or [3H]valine. The [14C]proline-labeled proteins were separated by chromatography on Agarose A-5 m in sodium dodecyl sulfate and their [14C]-hydroxypro...

journal_title：Connective tissue research

authors： Heeger P,Rosenbloom J

更新日期：1980-01-01 00:00:00

abstract：:The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...

journal_title：Connective tissue research

authors： Kao RT,Hall J,Stern R

更新日期：1986-01-01 00:00:00

abstract：:We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...

journal_title：Connective tissue research

authors： Fraser J,Lancaster GA,Scriver CR

更新日期：1983-01-01 00:00:00

abstract：:Hyperhomocysteinemia causes connective tissue pathology. Several theories on the mechanism of homocysteine toxicity in connective tissue are reviewed briefly. A possible new mechanism was revealed recently in the discovery of a reaction in which homocysteine thiolactone is converted to mercaptopropionaldehyde. The rea...

journal_title：Connective tissue research

authors： Toohey JI

更新日期：2008-01-01 00:00:00

abstract：:The resorption of devitalized bone by isolated osteoclasts in culture was studied by scanning electron microscopy. Osteoclasts attached to the bone and resorbed mineral but left most of matrix collagen undigested in the resorption pits. Postculture-treatment of bone substrate with collagenase completely removed the ma...

journal_title：Connective tissue research

authors： Shimizu H,Sakamoto S,Sakamoto M

更新日期：1989-01-01 00:00:00

abstract：:Bone morphogenetic protein-2 (BMP-2) is a multi-functional growth factor belonging to the transforming growth factor β superfamily that has a broad range of activities that affect many different cell types. BMP-2 induces odontoblastic differentiation of human dental pulp cells (DPCs), but the underlying mechanism rema...

journal_title：Connective tissue research

authors： Qin W,Liu P,Zhang R,Huang S,Gao X,Song Z,Wang R,Chen L,Guo B,Lin Z

更新日期：2014-06-01 00:00:00

abstract：:Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...

journal_title：Connective tissue research

authors： Matsushita T,Matsui N,Fujioka H,Kubo S,Kuroda R,Kurosaka M,Yoshiya S

更新日期：2004-01-01 00:00:00

abstract：:By using ultramorphological and biochemical methods, we analyzed the regional differences between the three parts of the equine superficial digital flexor tendon (SDFT), namely, the myotendinous junction (MTJ), middle metacarpal (mM), and osteotendinous junction (OTJ). Cross-sectional images showed unique distribution...

journal_title：Connective tissue research

authors： Watanabe T,Imamura Y,Hosaka Y,Ueda H,Takehana K

更新日期：2007-01-01 00:00:00

abstract：:The biological effects of isolated soluble dentin extracellular matrix components on the induction of reparative dentinogenesis in exposed cavities in ferret canine teeth have been shown to be blocked by immobilizing the extracellular matrix components on nitrocellulose or Millipore membranes during implantation. This...

journal_title：Connective tissue research

authors： Smith AJ,Tobias RS,Cassidy N,Bégue-Kirn C,Ruch JV,Lesot H

更新日期：1995-01-01 00:00:00

abstract：:Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...

journal_title：Connective tissue research

authors： Cho H,Lee S,Park SH,Huang J,Hasty KA,Kim SJ

更新日期：2013-01-01 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF) is associated with aberrant repair, persistence of collagen deposition, and the development of vascular remodeling. However, the role of angiogenesis in the pathogenesis of IPF is still undetermined. The aim of this study was to evaluate the combined mRNA expression of vascular endo...

journal_title：Connective tissue research

authors： Antoniou KM,Soufla G,Lymbouridou R,Economidou F,Lasithiotaki I,Manousakis M,Drositis I,Spandidos DA,Siafakas NM

更新日期：2010-01-01 00:00:00

abstract：:Purpose/Aim: Knee meniscus is a wedge-shaped fibrocartilaginous tissue, playing important roles in maintaining joint stability and function. Injuries to the meniscus, particularly with the avascular inner third zone, hardly heal and frequently progress into structural breakdown, followed by the initiation of osteoarth...

journal_title：Connective tissue research

authors： Tarafder S,Park G,Lee CH

更新日期：2020-05-01 00:00:00

abstract：:Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TI...

journal_title：Connective tissue research

authors： Nelson TR,Butkowski RJ,Michael AF,Charonis AS

更新日期：1997-01-01 00:00:00

abstract：:Transforming growth factor beta-1 (TGF beta-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, ...

journal_title：Connective tissue research

authors： Unterbrink A,O'Sullivan M,Chen S,MacDougall M

更新日期：2002-01-01 00:00:00

abstract：:To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PC...

journal_title：Connective tissue research

authors： Tanimoto K,Ohno S,Fujimoto K,Honda K,Ijuin C,Tanaka N,Doi T,Nakahara M,Tanne K

更新日期：2001-01-01 00:00:00