Abstract:
:In orthodontic tooth movement, prostaglandin E(2) (PGE(2)) released from osteoblasts can alter the normal process of bone remodeling. We examined the effect of compressive force (CF) on PGE(2) production, PGE receptors (Ep1-4) expression, phosphorylation of protein kinase A (p-PKA), and calcium content in Saos-2 cells. PGE(2) production increased as CF strength. Applying CF of 98 or 294 Pa caused the cells to produce approximately 700 and 1,400 pg/mL PGE(2), respectively. CF of 98 Pa increased Ep2 gene expression, and 98 and 294 Pa CF increased Ep4. Immunohistochemical staining showed strong expression of Ep2 under 98 Pa and Ep4 under 98 and 294 Pa. The p-PKA increased as the strength of CF or PGE(2) concentration. The calcium content was increased by the addition of 700 pg/mL PGE(2) but was decreased by 1400 pg/mL. Thus, mechanical stress controls bone formation by stimulating PGE(2) production and Ep2 and/or Ep4 expression in osteoblasts.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Sanuki R,Mitsui N,Suzuki N,Koyama Y,Yamaguchi A,Isokawa K,Shimizu N,Maeno Mdoi
10.1080/03008200701541775subject
Has Abstractpub_date
2007-01-01 00:00:00pages
246-53issue
5eissn
0300-8207issn
1607-8438pii
782091740journal_volume
48pub_type
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