Abstract:
:Studies were designed to test the hypothesis that plasma- and serum-deprived embryonic cells and tissues in vitro are capable of producing growth regulating factors which augment cartilage, bone and tooth induction during mouse mandibular process development. Embryonic mouse first branchial arch-derived mandibular processes (E11-E12, Theiler stages 18-19) or cap stage molar tooth (M1) organs (E15-E16, Theiler stage 23) expressed morphogenesis, histogenesis and cytodifferentiation (e.g., Meckel's cartilage and mandibular bone) when cultured as explants in permissive serumless and chemically-defined BGJB medium for periods up to 31 days in vitro. Organ cultures of early mandibular process explants in serumless conditions showed DNA synthesis comparable to the time- and position-restricted patterns characteristic for control in vivo development. As a paradigm for embryonic cell expression of putative growth factors, sense and antisense oligodeoxynucleotide probes corresponding to amino acids 1070-1081 for preproEGF, and antibodies directed against amino acids 348-691 of preproEGF, were used to identify and localize mRNA transcripts and translation products. Our preliminary evidence suggests that odontogenic epithelial and ectomesenchyme cells produce EGF-like products during instructive phases of tooth development. We suggest that plasma- and serum-deprived cells and tissues in vitro produce autocrine and/or paracrine growth factors which mediate embryonic mandibular morphogenesis, histogenesis and cytodifferentiation.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Slavkin HC,Sasano Y,Kikunaga S,Bessem C,Bringas P Jr,Mayo M,Luo W,Mak G,Rall L,Snead MLdoi
10.3109/03008209009152421subject
Has Abstractpub_date
1990-01-01 00:00:00pages
41-51issue
1eissn
0300-8207issn
1607-8438journal_volume
24pub_type
杂志文章,评审abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The mechanical properties of the human supraspinatus tendon (SST) are highly heterogeneous and may reflect an important adaptive response to its complex, multiaxial loading environment. However, these functional properties are associated with a location-dependent structure and composition that have not been fully eluc...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.847096
更新日期:2013-01-01 00:00:00
abstract::Cell cultures propagated from fetal bovine ligamentum nuchae were found to accumulate an extensive extracellular matrix containing collagen and elastic fibers when maintained for periods up to 8 weeks. The synthesis by these cells of two glycoproteins, designated MFP I and MFP II, which are immunologically related to ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152368
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mine...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1424149
更新日期:2018-12-01 00:00:00
abstract::Keratocan is an extracellular matrix protein that belongs to the small leucine-rich proteoglycan family that also includes lumican, biglycan, decorin, mimecan, and fibromodulin. Members of this family are known to play a role in regulating cellular processes such as proliferation and modulation of osteoprogenitor line...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2010.546536
更新日期:2011-10-01 00:00:00
abstract::Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1759562
更新日期:2020-05-05 00:00:00
abstract::Bone morphogenetic proteins (BMPs) are known to promote periodontal tissue regeneration, while noggin inhibits the biological activities of BMP-2, -4, and -7. To investigate the effect of BMPs and noggin gene transfer on cementogenesis, we used cloned murine cementoblasts (OCCM). Cells were transduced using adenovirus...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490278142
更新日期:2004-01-01 00:00:00
abstract::This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups wer...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600809935
更新日期:2006-01-01 00:00:00
abstract::Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact pr...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009811
更新日期:1990-01-01 00:00:00
abstract::An extensive literature on bone morphogenetic protein (BMP) induced generation and regeneration shows general agreement about one observation. The incidence and quantity of bone were greatest when BMP was delivered with a carrier of various biologic and non biologic polymers. In the present research, neutral lipids ei...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160210
更新日期:1997-01-01 00:00:00
abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029217
更新日期:1996-01-01 00:00:00
abstract::A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509013726
更新日期:1995-01-01 00:00:00
abstract::The purpose of this investigation is to evaluate enamel thickness in extant and extinct hominoids. The material used in this study spans the evolutionary history of this group, from 20 million years ago to the present. The objectives of this investigation are to test three hypotheses: (1) the Loading Hypothesis: loadi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809023927
更新日期:1998-01-01 00:00:00
abstract::Tubulointerstitial nephritis antigen (TIN-ag) is a recently described basement membrane component reactive with autoantibodies in some forms of autoimmune mediated tubulointerstitial nephritis. Immunofluorescence studies using polyclonal and monoclonal antibodies have indicated a restrictive tissue distribution for TI...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160222
更新日期:1997-01-01 00:00:00
abstract::Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase A...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709005621
更新日期:1987-01-01 00:00:00
abstract::The resorption of devitalized bone by isolated osteoclasts in culture was studied by scanning electron microscopy. Osteoclasts attached to the bone and resorbed mineral but left most of matrix collagen undigested in the resorption pits. Postculture-treatment of bone substrate with collagenase completely removed the ma...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023885
更新日期:1989-01-01 00:00:00
abstract::To extend previous studies on the apparent acceleration of collagen aging in diabetes mellitus, dura collagen from human adults of different ages, with and without diabetes, was processed to yield soluble and insoluble fractions. Insoluble fractions were cleaved by cyanogen bromide. Release of peptides from insoluble ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309004852
更新日期:1983-01-01 00:00:00
abstract::The pharmacokinetics of desmosine following a 5 mg intravenous dose was determined in five sheep. The biological half-life (t 1/2 beta) of desmosine varied between 81-489 min. Urinary recovery of unchanged desmosine indicated that the compound is not extensively metabolized and that the primary route of elimination is...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409013681
更新日期:1984-01-01 00:00:00
abstract::X-Ray diffraction and scanning electron microscopy show that collagen fibrils tend to be oriented parallel to the surface of the vertebral end plates in pig, rat and man. It is suggested that this orientation allows the end plate to withstand the pressure exerted on it by the nucleus pulposus. Scanning electron micros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109160244
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951440
更新日期:2014-10-01 00:00:00
abstract::Since hyaluronan anchors the proteoglycan-rich pericellular matrix to chondrocytes, hyaluronan-cell interactions may direct cartilage matrix assembly. To test this hypothesis, the competitive binding of hyaluronan hexasaccharides for native hyaluronan during matrix assembly, accumulation and retention in embryonic car...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609028895
更新日期:1996-01-01 00:00:00
abstract::For many years we have investigated the earliest crystal formations of different developing hard tissues (matrix vesicle, bone, dentine, enamel, etc.) by different electron microscopic measurements. It was observed that primarily Ca-phosphate (apatite) "chains," composed of nanometer sized particles (dots, islands), e...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008209509016998
更新日期:1995-01-01 00:00:00
abstract::Muscle-derived cells can differentiate into chondrogenic cells. In our present study, we investigated the pattern of expression of Sox9, a transcription factor known to play a key role in chondrogenesis, in a rat myoblastic cell line, L6. In addition, we evaluated expression of type II collagen and myogenic regulatory...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490514130
更新日期:2004-01-01 00:00:00
abstract::The basic subunit of cartilage proteoglycan consists of multiple glycosaminoglycan chains covalently attached to a core protein. It is unclear as to whether there is a single core protein or multiple different core proteins, since previous studies using either chondroitinase or testicular hyaluronidase to enzymaticall...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207709152265
更新日期:1977-01-01 00:00:00
abstract::Purpose/Aim: Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from vascular smooth muscle cells significantly contribute to the initiation and progression of this pathology. Recently, we have demonstrated that elevated p...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1444759
更新日期:2018-12-01 00:00:00
abstract::We previously reported that circulating peripheral blood-borne cells (PBCs) contribute to early-phase meniscal reparative change. Because macrophages and myofibroblasts are important contributors of tissue regeneration, we examined their origin and distribution in the reparative meniscus. Reparative menisci were evalu...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1284825
更新日期:2017-11-01 00:00:00
abstract::Proteoglycans extracted from articular cartilage of large joints of humans aged 4, 11, 70 and 75, were fractionated on associative density gradients. The top fraction (A3) was purified by ion-exchange chromatography and subsequent gel filtration on Sepharose CL 4B in 4 M GuCl, 0.5% Triton x 100. Proteoglycans from you...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809017475
更新日期:1988-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract:PURPOSE:Treatment of meniscal injury is important for osteoarthritis (OA) prevention. Meniscus cells are divided between inner and outer cells, which have different characteristics and vascularity. We evaluated the effects of hyaluronic acid (HA) on the proliferation and migration of human inner and outer meniscus cell...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1465053
更新日期:2019-03-01 00:00:00
abstract::Cells derived from organ-explant culture of the cartilaginous component of osteochondrophytic spurs of human femoral heads were incubated with [35S]-sulfate in order to study sulfated proteoglycan biosynthesis in vitro. Secondary monolayer cultures incorporated [35S]-sulfate into macromolecules which were recovered in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208409013694
更新日期:1984-01-01 00:00:00