Abstract:
:Changes in the structure and organization of collagen fibrils were recently described in the skin of aspartylglycosaminuria patients. The skin of the patients contained a normal amount and distribution of glycosaminoglycans, but the dermatan sulfate of aspartylglycosaminuria skin was more sensitive to chondroitinase AC digestion, resulting in unsaturated 4-sulfated disaccharides which were not detected in controls. Isolated dermatan sulfate chains as well as the chains present in the intact core protein synthesized by skin fibroblasts from an aspartylglycosaminuria patient were also digestible with chondroitinase AC, while those of a control fibroblast culture could be digested with chondroitinase ABC only. This is indirect evidence for abnormal epimerization of dermatan sulfate in the skin of aspartylglycosaminuria patients, which may be associated with the changes in collagen fibril formation.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Näntö-Salonen K,Larjava H,Säämanen AM,Heino J,Penttinen R,Pelliniemi LJ,Tammi Mdoi
10.3109/03008208709005621subject
Has Abstractpub_date
1987-01-01 00:00:00pages
367-76issue
4eissn
0300-8207issn
1607-8438journal_volume
16pub_type
杂志文章abstract::A high frequency restriction fragment length polymorphism (RFLP) at the 3'-end of the pigeon pro alpha 2(1) collagen gene was detected using the restriction endonuclease EcoR1. The distribution of this allelic variant was analyzed in DNA isolated from White Carneau pigeons genetically susceptible to the development of...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152437
更新日期:1991-01-01 00:00:00
abstract::Fitting children and infants who have osteogenesis imperfecta (OI) with braces has posed substantial problems of implementation and patient management For the past twelve years bracing has been an important component of a patient research and management program conducted at the National Institutes of Health. By using ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509116832
更新日期:1995-01-01 00:00:00
abstract::The fibrous collagens form the structural basis of all mammalian connective tissues, including the vasculature, dermis, bones, tendons, cartilage, and those tissues that support organs such as the heart, kidneys, liver, and lungs. The helical structure of collagen has been extensively studied but in addition to its he...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.3109/03008207.2010.511353
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:The presence of genetic variants in uric acid (UA) transporters can be associated with hyperuricemia, and therefore with an increased risk of monosodium urate (MSU) crystal precipitation. The inflammatory process triggered by these crystals leads to cartilage damage, which, in turn, could promote knee osteoa...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1483359
更新日期:2019-05-01 00:00:00
abstract::Growth factors were historically defined as molecules produced by the body to regulate cell growth and proliferation. After the identification of their receptors and the intracellular signaling machinery they activate, it is now clear that they are involved in the regulation of multiple processes essential for develop...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200802148629
更新日期:2008-01-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::By using ultramorphological and biochemical methods, we analyzed the regional differences between the three parts of the equine superficial digital flexor tendon (SDFT), namely, the myotendinous junction (MTJ), middle metacarpal (mM), and osteotendinous junction (OTJ). Cross-sectional images showed unique distribution...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200701692800
更新日期:2007-01-01 00:00:00
abstract::Odontogenesis involves a complex series of processes including epithelial-mesenchymal interactions, morphogenesis, differentiation, fibrillogenesis, and mineralization. Extracellular (ECM) remodeling plays a critical role in the rapid morphological changes that accompany these events. It is proposed that matrix metall...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200290000538
更新日期:2002-01-01 00:00:00
abstract::To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model. Specifically, immunohis...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209809021505
更新日期:1998-01-01 00:00:00
abstract::To study the glycosylation of glomerular basement membrane collagen (GBMC) in diabetes, kidneys were obtained at autopsy from 5 patients with insulin-requiring diabetes of long duration and diabetic complications, and from 5 control subjects. Glomeruli were prepared by sieving and collagen was isolated by limited peps...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008054
更新日期:1982-01-01 00:00:00
abstract::During cloning of the rat incisor phosphophoryn gene, several clones were identified with a PP antibody. One of the clones (2PP) was shown to encode a PP denoted as dentin matrix protein 2, DMP2. We have now sequenced another clone, which appears to be bifunctional, or at minimum, has two distinct domains. The 5' regi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209909005277
更新日期:1999-01-01 00:00:00
abstract::Primary chondrocytes cultured in agarose can escape the gel, accumulate at the interface between agarose and the culture medium, and form an outgrowing tissue. These outgrowths can appear as voluminous cartilage-like nodules that have never been previously investigated. In the present study, bovine articular chondrocy...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200903281683
更新日期:2010-06-01 00:00:00
abstract::Osteoarthritis (OA) is typically managed in late stages by replacement of the articular cartilage surface with a prosthesis as an effective, though undesirable outcome. As an alternative, hydrogel implants or growth factor treatments are currently of great interest in the tissue engineering community, and scaffold mat...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1698556
更新日期:2020-05-01 00:00:00
abstract::The efficacy of connective tissue explants is difficult to determine, particularly where autopsy material is required for research or clinical applications. We report here an optimised protocol using 5-chloromethylfluorescein diacetate (CMFDA) and ethidium homodimer-1 to distinguish viable and non-viable cells in a ra...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609028880
更新日期:1996-01-01 00:00:00
abstract:UNLABELLED:Keratocystic odontogenic tumors (KCOTs) are locally invasive, rapidly proliferating cystic lesions of the jaw. The bone-invasive nature of these tumors has been previously associated with the expression of matrix metalloproteinases (MMPs), which degrade the extracellular matrix. The purpose of this study was...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923875
更新日期:2014-08-01 00:00:00
abstract::Activation of protein kinase C (PKC) can upregulate tooth eruption molecules such as osteoprotegerin (OPG) and vascular endothelial growth factor (VEGF). In this study, we examined the in vivo gene expression of classic isoforms of PKC in the dental follicle of postnatal rats. The expression level of PKC-alpha was sig...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200490882628
更新日期:2004-01-01 00:00:00
abstract::We examined the effects of several variables on the ratio of type I:type III collagen secreted by human caucasian skin fibroblasts in normal and osteogenesis imperfecta (OI) phenotypes. Isotopically labelled collagen extracted from fibroblast medium was analyzed by DEAE-cellulose chromatography and identified by appro...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208309015011
更新日期:1983-01-01 00:00:00
abstract::The desmoplastic response to breast carcinoma is being studied. The stimulation of stromal cell proliferation by a preformed breast tumor matrix was shown. An additional mechanism for stimulating scleroprotein deposition is described here. On a per-cell basis, the synthesis of collagen and elastin was increased by 50%...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609017468
更新日期:1986-01-01 00:00:00
abstract::Objective: Interleukin-17 (IL-17), produced by T helper (Th)-17 cells, is a potent regulator of bone homeostasis. Osteoblasts are key cells that orchestrate inflammatory bone destruction and bone remodeling. This study examines the effect of different concentrations of IL-17 on osteogenesis and receptor activator of n...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2020.1759562
更新日期:2020-05-05 00:00:00
abstract:OBJECTIVE:Excessive apoptosis plays an important role in the progression of intervertebral disc degeneration. However, the effect of autophagy, another type of programmed cell death, on the pathogenesis of disc degeneration is still unclear. Macroautophagy and chaperone-mediated autophagy (CMA) change and intervertebra...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2011.564336
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS:We hypothesized that a partial-width injury to the attachment would heal via fibros...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2018.1485666
更新日期:2018-09-01 00:00:00
abstract::Although intervertebral disc (IVD) degeneration is one of most common causes of morbidity, its etiology remains unclear. In healthy discs, the rates of synthesis and breakdown of the extracelluar matrix (ECM) are in equilibrium because of intricate regulation by growth factors and catabolic cytokines. Important among ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.775258
更新日期:2013-01-01 00:00:00
abstract::Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:We investigated whether COMP may modify cartilage metabolism and play a role as an endogenous disease aggravating factor in OA. MATERIALS AND METHODS:Full-length and momomeric COMP was recombinantly expressed in human embryonic kidney cells and purified it via affinity chromatography. Purified COMP was used ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.951440
更新日期:2014-10-01 00:00:00
abstract::The interaction of sodium dodecyl sulfate with elastin has been studied by complexometric titration. Approximately 1.2 mumoles of protons with a pKapp of 5.45 are taken up by 10 milligrams of insoluble elastin upon the binding of detergent, apparently due to the protonation of normally ionized carboxylate functions in...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152627
更新日期:1978-01-01 00:00:00
abstract::The mechanical properties of collagen as a biopolymer ensures that collagen has a significant influence on the mechanical behavior of the host tissue. Structural organization is a key to that influence. We have assessed this relationship quantitatively in the tunica media of arteries from the heart and brain, using th...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152168
更新日期:1991-01-01 00:00:00
abstract::The interaction of elastin and microfibrils in elastogenesis in vitro was investigated with electron microscopy and immunohistochemistry. Fetal human pulmonary fibroblasts were cultured with or without beta-aminopropionitrile (BAPN). One week after seeding, the extracellular microfibrils were loosely arranged without ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209309016835
更新日期:1993-01-01 00:00:00
abstract::The effect of I- and C1- on the enzymatic degradation of elastin pancreatic elastase has been studied. At low halide concentrations both ions show a marked stimulation of elastolysis, I- being significantly more effective than Cl-. Kinetic plots of enzyme activity indicate that anions enhance the susceptibility of the...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152616
更新日期:1978-01-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) is a ubiquitous regulator of cellular growth and differentiation. The present study investigated the effects of TGF-beta on chick growth plate chondrocyte proliferation, matrix synthesis, and alkaline phosphatase activity in short term cultures. TGF-beta markedly stimulated D...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208909023900
更新日期:1989-01-01 00:00:00
abstract::Bone and dentin contain many phosphoproteins in their extracellular matrix. It has been postulated that the anionic character of these proteins, and the presence of phosphate groups in particular, is important for various functions related to biomineralization. Phosphophoryns (PP), are the most highly phosphorylated d...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029194
更新日期:1996-01-01 00:00:00