Abstract:
:Effects of applied hydrostatic pressure on transmembrane potentials were investigated in sheep articular chondrocytes and human skin fibroblasts in non-confluent monolayer cultures. Resting potentials in chondrocytes (about -12 mv) and in fibroblasts (about -15 mV) were increased and decreased respectively by over 40% after pressure was applied cyclically (0.33 Hz, 120 mm Hg, 20 minutes). Continuous pressure (120 mm Hg, 20 minutes) caused deplorization in both cell types. Low frequency pressure application (less than 0.08 Hz) caused depolarization in chondrocytes and hyperpolarization in fibroblasts. Quinidine (2 x 10(-5) M) blocked and verapamil (10(-5) M) reduced hyperpolarization responses, suggesting involvement of Ca(2+)-dependent K+ channels. A23187 (1.9 x 10(-6) M) caused hyperpolarization in chondrocytes, augmented further by subsequent pressure application (0.33 Hz). Tetrodotoxin (10(-6) M) blocked depolarization responses indicating that these were due to Na+ influx. Blockade of histamine H1 receptors by chlorpheniramine maleate (5.1 x 10(-6) M), H2 receptors by cimetidine (7.9 x 10(-6) M) and beta-adrenoreceptors by sotolol (1.3 x 10(-4) M) had no effect on hydrostatic pressure-induced hyperpolarization in chondrocytes. Cytochalasin B (2 x 10(-5) M and at 4 x 10(-6) M) abolished pressure-induced hyperpolarization in chondrocytes; in contrast, applied cyclical hydrostatic pressure to cytochalasin-treated fibroblasts caused hyper-polarization, suggesting that cytoskeletal changes were involved.
journal_name
Connect Tissue Resjournal_title
Connective tissue researchauthors
Wright MO,Stockwell RA,Nuki Gdoi
10.3109/03008209209014227keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
49-70issue
1-2eissn
0300-8207issn
1607-8438journal_volume
28pub_type
杂志文章abstract::The connective tissue protein elastin is largely responsible for maintaining the elasticity of major blood vessels and lung tissue. Comparatively few studies have been made of the immunologic properties of elastin because of its high degree of insolubility and because of its apparent limited capacity to elicit precipi...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152131
更新日期:1981-01-01 00:00:00
abstract::Anterior cruciate ligament (ACL) has poor healing ability and an injured ACL would induce the degeneration of other intra-articular connective tissues. However, the coordinated expression and activities of matrix metalloproteinase (MMPs) in intra-articular tissues induced by ACL rupture were poorly understood. With a ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Gene expression of osteopontin (OPN) has been investigated in mice by application of laser capture microdissection (LCM) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. LCM permits individual cells to be isolated ("captured") from tissue sections for molecular analyses. In this study, chondrocyt...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro. The experiments were performed using a previously described culture ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209609029206
更新日期:1996-01-01 00:00:00
abstract::Oriented fibers, arising from the self assembly of collagen, may be produced from in vitro fibrillogenesis of soluble collagen with gradients of fibrillogenic potential. These gradients may be introduced by either concentration gradients of pronase-treated collagen or of arginine which, in turn, create concentration g...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208808992792
更新日期:1988-01-01 00:00:00
abstract::Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923851
更新日期:2014-08-01 00:00:00
abstract::The effects of two forms of elastase on the Young's Modulus of elastic tissue have been studied. The monomeric, calcium poor form of the enzyme lowers the value of the Young's Modulus before an appreciable amount of protein is taken into solution. The polymeric, calcium rich enzyme on the other hand degrades the prote...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207609152217
更新日期:1976-01-01 00:00:00
abstract::Although bone is a nanocomposite of mineral and collagen, mineral has been the more elusive component of study. A standard for bone mineral is clearly needed. We hypothesized that the most natural, least-processed bone mineral could be retrieved from the most highly mineralized bone. We therefore studied the rostrum o...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2013.769973
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:Excessive apoptosis plays an important role in the progression of intervertebral disc degeneration. However, the effect of autophagy, another type of programmed cell death, on the pathogenesis of disc degeneration is still unclear. Macroautophagy and chaperone-mediated autophagy (CMA) change and intervertebra...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2011.564336
更新日期:2011-01-01 00:00:00
abstract::Proteoglycans extracted from articular cartilage of large joints of humans aged 4, 11, 70 and 75, were fractionated on associative density gradients. The top fraction (A3) was purified by ion-exchange chromatography and subsequent gel filtration on Sepharose CL 4B in 4 M GuCl, 0.5% Triton x 100. Proteoglycans from you...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208809017475
更新日期:1988-01-01 00:00:00
abstract::An extensive literature on bone morphogenetic protein (BMP) induced generation and regeneration shows general agreement about one observation. The incidence and quantity of bone were greatest when BMP was delivered with a carrier of various biologic and non biologic polymers. In the present research, neutral lipids ei...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209709160210
更新日期:1997-01-01 00:00:00
abstract::Purpose: Transplantation of autologous and/or allogeneic blood vessels is the most convenient treatment for vascular diseases. With regard to extensive need for blood vessels, developments in vascular tissue engineering are contributing greatly. In this study, our aim is to create intact small-diameter tubular vascula...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2019.1651848
更新日期:2021-03-01 00:00:00
abstract::Bone sialoprotein (BSP) expression is restricted to cells associated with the mineralization of bones and teeth. We previously identified a homeodomain binding element in a 2.5 kb fragment of the murine Bsp promoter that is required for osteoblast-selective expression in cell culture. To examine the role of this eleme...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Part of the hyaluronic acid (HA) synthesized in peripheral tissues enters the blood circulation through the lymph. It is rapidly taken up by the endothelial cells in the liver (half-life in blood is 2.5-5.5 minutes) and degraded. Pure primary cultures of liver endothelial cells were obtained by a newly developed techn...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208609001971
更新日期:1986-01-01 00:00:00
abstract::This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups wer...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008200600809935
更新日期:2006-01-01 00:00:00
abstract::White Carneau pigeons have previously been shown to be genetically susceptible to the development of spontaneous atherogenesis. The severity of development of atheromatous lesions is considerably greater than a more resistant breed of Show Racer pigeons. Analysis of levels of total hydroxyproline and isodesmosine in t...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209009009813
更新日期:1990-01-01 00:00:00
abstract::Parietal yolk sacs isolated from 14.5-day rat embryos and incubated in vitro with either [14C]proline, [3H]mannose or 3H-labeled amino acid mixture synthesized and secreted basement membrane collagenous and noncollagenous glycoprotein components with relative molecular weights of 350,000 (350K), 220,000 (220K), 185,00...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209008056
更新日期:1982-01-01 00:00:00
abstract::The crystal ghosts represent a crystal associated organic material which is stained by acidic phosphotungstic acid, periodic acid-silver methenamine and periodic acid-thiosemicarbazide-osmium, and is reactive with cations and with colloidal iron at pH 2.0, and unreactive after methylation and saponification. These res...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. One of these was found to have similar molecular ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209509010814
更新日期:1995-01-01 00:00:00
abstract::This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovarie...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008200902855917
更新日期:2010-01-01 00:00:00
abstract::The effects of various avocado oils on collagen metabolism in skin were studied in growing rats fed diets containing 10% (w/w) of the tested oils. Rats fed the unrefined avocado oil extracted with hexane from the intact fruit, its unsaponifiables or the avocado seed oil, showed significant increases in soluble collage...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109152159
更新日期:1991-01-01 00:00:00
abstract::The diameter of collagen fibrils has been measured in electron micrographs of the tunica media and adventitia of the common carotid artery of 14 rats (19,262 counts). The fibrils of the media were measured in each of its three interlamellar spaces. The mean fibril diameters in the innermost space and the middle space ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208709002000
更新日期:1987-01-01 00:00:00
abstract::A homogeneous preparation of glycoprotein (apparent molecular weight 34,000) has been isolated from bovine ligamentum nuchae by treatment of the tissue with 5 M guanidine HCl and 2-mercaptoethanol. The preparation exhibits amine oxidase activity which is only marginally inhibited by beta-aminopropionitrile. ...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208109152380
更新日期:1981-01-01 00:00:00
abstract::Hyperhomocysteinemia causes connective tissue pathology. Several theories on the mechanism of homocysteine toxicity in connective tissue are reviewed briefly. A possible new mechanism was revealed recently in the discovery of a reaction in which homocysteine thiolactone is converted to mercaptopropionaldehyde. The rea...
journal_title:Connective tissue research
pub_type: 杂志文章,评审
doi:10.1080/03008200801913742
更新日期:2008-01-01 00:00:00
abstract::Sea urchin's teeth from four families of order Echinoida and from orders Temnopleuroida, Arbacioida and Cidaroida were studied with synchrotron X-ray diffraction. The high and very high Mg calcite phases of the teeth, i.e. the first and second stage mineral constituents, respectively, have the same crystallographic or...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207.2014.923865
更新日期:2014-08-01 00:00:00
abstract::The total and individual glycosaminoglycan (GAG) content at various stages of the Dupuytren disease process and in samples of normal palmar connective tissue (palmar dermis, palmar fascia and digital flexor tendon) from the hands of uninvolved age-matched controls have been assayed and compared. Morphological comparis...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160258
更新日期:1982-01-01 00:00:00
abstract::We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008209109006995
更新日期:1991-01-01 00:00:00
abstract::A method is described for accurate and reproducible determination of wet and dry weights of small articular cartilage samples. The water content of the articular cartilage samples before and after treatment with selective degradative enzymes and "artificial fibrillation" was determined. It was found that the water con...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008208209160266
更新日期:1982-01-01 00:00:00
abstract:OVERVIEW:Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance; however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of PO...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.1080/03008207.2017.1306060
更新日期:2018-03-01 00:00:00
abstract::Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular...
journal_title:Connective tissue research
pub_type: 杂志文章
doi:10.3109/03008207809152287
更新日期:1978-01-01 00:00:00