Therapeutic ultrasound stimulation of tendon cell migration.

Abstract:

:Ultrasound is a therapeutic agent commonly used to treat sports-related tendinopathy. Tendon healing requires tendon cells migration to the repair site, followed by the proliferation and synthesis of extracellular matrix. This study was designed to determine the effect of ultrasound on migration of tendon cells intrinsic to rat Achilles tendon. Furthermore, the existence of a correlation between this effect and the expression of the contractile actin isoform, alpha-smooth muscle (SM) actin, which is associated with cell mobility, was also examined. Cell migration was evaluated by transwell filter migration assay. The mRNA expressions of alpha-SM actin were determined by reverse transcription-polymerase chain reaction. Dose-dependent ultrasound enhancement of tendon cells migration through the transwell filter was demonstrated. Using immunofluorescence stain for alpha-SM actin, the percentages of alpha-SM actin-positive cells of total cells, nonmigrated cells, and migrated cells on the filter were calculated. Ultrasound-treated cells which had migrated to the bottom side of the filter were more likely to express alpha-SM actin than migrated control cells and nonmigrated cells. However, there was no change of mRNA and protein expression of alpha-SM actin as well as expression of FAK and p-FAK. In conclusion, ultrasound stimulates tendon cell migration in association with increased expression of alpha-SM actin of tendon cells.

journal_name

Connect Tissue Res

authors

Tsai WC,Chen JY,Pang JH,Hsu CC,Lin MS,Chieh LW

doi

10.1080/03008200802325359

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

367-73

issue

5

eissn

0300-8207

issn

1607-8438

pii

905233466

journal_volume

49

pub_type

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