Abstract:
BACKGROUND:Classical swine fever virus (CSFV) is the member of the genus Pestivirus under the family Flaviviridae. The 5' untranslated region (UTR) of CSFV contains the IRES, which is a highly structured element that recruits the translation machinery. The 3' UTR is usually the recognition site of the viral replicase to initiate minus-strand RNA synthesis. Adenosine-uridine rich elements (ARE) are instability determinants present in the 3' UTR of short-lived mRNAs. However, the presence of AREs in the 3' UTR of CSFV conserved in all known strains has never been reported. This study inspects a possible role of the ARE in the 3' UTR of CSFV. RESULTS:Using RNA pull-down and LC/MS/MS assays, this study identified at least 32 possible host factors derived from the cytoplasmic extracts of PK-15 cells that bind to the CSFV 3' UTR, one of which is HuR. HuR is known to bind the AREs and protect the mRNA from degradation. Using recombinant GST-HuR, this study demonstrates that HuR binds to the ARE present in the 3' UTR of CSFV in vitro and that the binding ability is conserved in strains irrespective of virulence. CONCLUSIONS:This study identified one of the CSFV 3' UTR binding proteins HuR is specifically binding to in the ARE region.
journal_name
Virol Jjournal_title
Virology journalauthors
Nadar M,Chan MY,Huang SW,Huang CC,Tseng JT,Tsai CHdoi
10.1186/1743-422X-8-340subject
Has Abstractpub_date
2011-07-06 00:00:00pages
340issn
1743-422Xpii
1743-422X-8-340journal_volume
8pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be required when HR fails. Previously, in vitro ligation has been suggested to faci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-18
更新日期:2009-02-07 00:00:00
abstract:BACKGROUND:Kadipiro virus (KDV) belongs to the Reoviridae family, which consists of segmented, non-enveloped, double-stranded RNA viruses. It has previously been isolated from Culex, Anopheles, Armigeres and Aedes mosquitoes in Indonesia and China. Here, we describe the isolation and characterization of SDKL1625 from A...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-0966-y
更新日期:2018-04-06 00:00:00
abstract:BACKGROUND:Otitis media (OM) affects ≥80% of children before the age of three. OM can arise following co-infection with influenza A virus (IAV) and the bacterium Streptococcus pneumoniae. We have previously shown that H3 IAV strains (such as Udorn/72) induced a higher rate of bacterial OM than H1 strains (such as PR8/3...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-128
更新日期:2013-04-23 00:00:00
abstract:BACKGROUND:Jamestown Canyon virus (JCV), family Bunyaviridae, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-136
更新日期:2011-03-24 00:00:00
abstract:BACKGROUND:Due to the high prevalence of viral infections having no specific treatment and the constant appearance of resistant viral strains, the development of novel antiviral agents is essential. The aim of this study was to evaluate the antiviral activity against bovine viral diarrhea virus, herpes simplex virus ty...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-245
更新日期:2013-07-27 00:00:00
abstract::Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral r...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-7
更新日期:2005-02-10 00:00:00
abstract:BACKGROUND:Mumps virus is a negative-sense, single stranded RNA virus consisting of a ribonucleocapsid core enveloped by a lipid membrane derived from host cell, which causes mumps disease preventable by vaccination. Since virus lipid envelope and glycosylation pattern are not encoded by the virus but dependent on the ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0463-0
更新日期:2016-01-14 00:00:00
abstract:BACKGROUND:Aleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to vali...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-141
更新日期:2014-08-08 00:00:00
abstract::The I5L gene is one of approximately 90 genes that are conserved throughout the chordopoxvirus family, and hence are presumed to play vital roles in the poxvirus life cycle. Previous work had indicated that the VP13 protein, a component of the virion membrane, was encoded by the I5L gene, but no additional studies had...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-148
更新日期:2008-12-15 00:00:00
abstract:BACKGROUND:Lassa hemorrhagic fever (LHF) is a neglected tropical disease with significant impact on the health care system, society, and economy of Western and Central African nations where it is endemic. With a high rate of infection that may lead to morbidity and mortality, understanding how the virus interacts with ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-306
更新日期:2010-11-09 00:00:00
abstract:BACKGROUND:Herpes simplex viruses (HSVs) rapidly shut off macromolecular synthesis in host cells. In contrast, global microarray analyses have shown that HSV infection markedly up-regulates a number of host cell genes that may play important roles in HSV-host cell interactions. To understand the regulatory mechanisms i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-257
更新日期:2011-05-25 00:00:00
abstract::Lytic bacteriophage 0305phi8-36 forms visually observed aggregates during plaque formation. Aggregates intrinsically lower propagation potential. In the present study, the following observations indicate that lost propagation potential is regained with time: (1) Aggregates sometimes concentrate at the edge of clear pl...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-131
更新日期:2007-12-04 00:00:00
abstract:BACKGROUND:Avian influenza virus (AIV) is classified into two pathotypes, low pathogenic (LP) and high pathogenic (HP), based on virulence in chickens.Differences in pathogenicity between HPAIV and LPAIV might eventually be related to specific characteristics of strains, tissue tropism and host responses. METHODS:To s...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-61
更新日期:2012-03-06 00:00:00
abstract::A series of frameshift mutations within the 3a gene has been observed in culture-derived severe acute respiratory syndrome coronavirus (SARS-CoV). We report here that viral RNA from clinical samples obtained from SARS-CoV infected patients also contains a heterogeneous population of wild-type and mutant 3a transcripts...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-51
更新日期:2005-06-20 00:00:00
abstract::Bats have been recognized as the natural reservoirs of a large variety of viruses. Special attention has been paid to bat coronaviruses as the two emerging coronaviruses which have caused unexpected human disease outbreaks in the 21st century, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Re...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-015-0422-1
更新日期:2015-12-22 00:00:00
abstract:BACKGROUND:Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked glycan on the "glycan loop...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01313-1
更新日期:2020-03-31 00:00:00
abstract:BACKGROUND:Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. METHODS:The expression...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1220-y
更新日期:2019-10-07 00:00:00
abstract:BACKGROUND:The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive samplin...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01298-x
更新日期:2020-03-05 00:00:00
abstract:BACKGROUND:Due to potential advantages, human adenoviral vectors have been evaluated pre-clinically as recombinant vaccine vectors against several cancers and infectious diseases, including human immunodeficiency virus (HIV) infection. The V3 loop of HIV-1 glycoprotein 120 (gp120) contains important neutralizing epitop...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-112
更新日期:2014-06-16 00:00:00
abstract::Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cro...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-482
更新日期:2011-10-27 00:00:00
abstract:BACKGROUND/AIM:Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease has emerged. HCV circulates in vivo as a complex population of different but closely related viral variants, commonly referred to as a quasispecies. The extent to which recombina...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-87
更新日期:2006-10-24 00:00:00
abstract:BACKGROUND:There are over 100 known human adenovirus (HAdV) types, which are able to cause a broad variety of different self-limiting but also lethal diseases especially in immunocompromised patients. Only limited information about the pathogenesis and biology of the majority of these virus types is available. In the p...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01318-w
更新日期:2020-04-09 00:00:00
abstract:BACKGROUND:Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects. METHODS:In this...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1266-x
更新日期:2019-12-23 00:00:00
abstract:BACKGROUND:Containment of influenza A H1N1 virus spread was implemented successfully in Xiamen, with large-scale inoculation to reduce morbidity. To identify beneficial elements and to guide decision-making in epidemic containment, we analyzed the epidemiologic parameters and evaluated the control measures. METHOD:We ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-20
更新日期:2012-01-17 00:00:00
abstract:BACKGROUND:Farmington virus (FARV) is a rhabdovirus that was isolated from a wild bird during an outbreak of epizootic eastern equine encephalitis on a pheasant farm in Connecticut, USA. FINDINGS:Analysis of the nearly complete genome sequence of the prototype CT AN 114 strain indicates that it encodes the five canoni...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-219
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:To understand the prevalence and characteristics of human cytomegalovirus (HCMV) infection in children in the Weifang area, and to provide information for its prevention and treatment. METHODS:A comprehensive survey was performed from 2009 to 2012 in 7582 children from birth to 6 years of age hospitalized i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-42
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0353-x
更新日期:2015-08-08 00:00:00
abstract:BACKGROUND:Influenza viruses are characterized by their highly variable surface proteins HA and NA. The third surface protein M2 is a nearly invariant protein in all Influenza A strains. Despite extensive studies in other animal models, this study is the first to describe the use of recombinant M2 protein and a peptide...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-206
更新日期:2013-06-24 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to prevent chronic infection is important. We reported earlier that a re...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-118
更新日期:2010-06-07 00:00:00
abstract:BACKGROUND:Preeclampsia is a major health problem. Although, the pathophysiology of preeclampsia is not fully understood, there are recent studies on association between infections and preeclampsia. OBJECTIVE:The aim of the present study was to investigate the association between maternal seropositivity of rubella, He...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0813-6
更新日期:2017-07-26 00:00:00