Investigation of the immunogenicity of Zika glycan loop.

Abstract:

BACKGROUND:Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked glycan on the "glycan loop" (GL) of the ZIKV envelope protein protects the functionally important "fusion loop" on the opposite E subunit in the dimer, and EDE antibodies have been shown to bind to both of these loops. Human EDE antibodies have been divided into two subclasses based on how they bind to the glycan loop region: EDE1 antibodies do not require glycosylation for binding, while EDE2 antibodies strongly rely on the glycan for binding. METHODS:ZIKV GL was expressed on tobacco mosaic virus nanoparticles. Mice were immunized with GL or full-length monomeric E and the immune response was analyzed by testing the ability of sera and monoclonal antibodies to bind to GL and to neutralize ZIKV in in vitro cellular assay. RESULTS:We report here the existence of ZIKV moderately neutralizing antibodies that bind to E monomers through epitopes that include the glycan loop. We show that sera from human Zika patients contain antibodies capable of binding to the unglycosylated glycan loop in the absence of the rest of the envelope protein. Furthermore, mice were inoculated with recombinant E monomers and produced neutralizing antibodies that either recognize unglycosylated glycan loop or require glycan for their binding to monomeric E. We demonstrate that both types of antibodies neutralize ZIKV to some extent in a cellular virus neutralization assay. CONCLUSIONS:Analogous to the existing EDE antibody nomenclature, we propose a new classification for antibodies that bind to E monomer epitopes (EME): EME1 and EME2 for those that do not require and those that do require glycan for binding to E, respectively.

journal_name

Virol J

journal_title

Virology journal

authors

Henderson EA,Tam CC,Cheng LW,Ngono AE,Nguyen AV,Shresta S,McGee M,Padgett H,Grill LK,Martchenko Shilman M

doi

10.1186/s12985-020-01313-1

subject

Has Abstract

pub_date

2020-03-31 00:00:00

pages

43

issue

1

issn

1743-422X

pii

10.1186/s12985-020-01313-1

journal_volume

17

pub_type

杂志文章
  • Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses.

    abstract:BACKGROUND:The genus Flavivirus includes several pathogenic agents that cause severe illness in humans. Re-emergence of West Nile virus in Europe and continuous spread of certain flaviviruses such as dengue, yellow fever and Japanese encephalitis viruses represent a global danger to public health. Therefore, a rapid an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-58

    authors: Patel P,Landt O,Kaiser M,Faye O,Koppe T,Lass U,Sall AA,Niedrig M

    更新日期:2013-02-14 00:00:00

  • Biochemical typing of pathological prion protein in aging cattle with BSE.

    abstract:BACKGROUND:The broad enforcement of active surveillance for bovine spongiform encephalopathy (BSE) in 2000 led to the discovery of previously unnoticed, atypical BSE phenotypes in aged cattle that differed from classical BSE (C-type) in biochemical properties of the pathological prion protein. Depending on the molecula...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-64

    authors: Tester S,Juillerat V,Doherr MG,Haase B,Polak M,Ehrensperger F,Leeb T,Zurbriggen A,Seuberlich T

    更新日期:2009-05-26 00:00:00

  • Interactions of viruses in Cowpea: effects on growth and yield parameters.

    abstract::The study was carried out to investigate the effects of inoculating three cowpea cultivars: "OLO II", "OLOYIN" and IT86D-719 with three unrelated viruses: Cowpea aphid-borne mosaic virus (CABMV), genus Potyvirus, Cowpea mottle virus (CMeV), genus Carmovirus and Southern bean mosaic virus (SBMV), genus Sobemovirus sing...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-15

    authors: Kareem KT,Taiwo MA

    更新日期:2007-02-08 00:00:00

  • New insights regarding HCV-NS5A structure/function and indication of genotypic differences.

    abstract:BACKGROUND:HCV is prevalent throughout the world. It is a major cause of chronic liver disease. There is no effective vaccine and the most common therapy, based on Peginterferon, has a success rate of ~50%. The mechanisms underlying viral resistance have not been elucidated but it has been suggested that both host and ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-14

    authors: Yamasaki LH,Arcuri HA,Jardim AC,Bittar C,de Carvalho-Mello IM,Rahal P

    更新日期:2012-01-12 00:00:00

  • Bat origin of human coronaviruses.

    abstract::Bats have been recognized as the natural reservoirs of a large variety of viruses. Special attention has been paid to bat coronaviruses as the two emerging coronaviruses which have caused unexpected human disease outbreaks in the 21st century, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and Middle East Re...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/s12985-015-0422-1

    authors: Hu B,Ge X,Wang LF,Shi Z

    更新日期:2015-12-22 00:00:00

  • New Vibrio cholerae O1 biotype ElTor bacteriophages.

    abstract::We report the presence of three new O1 ElTor vibriophages named AS1, AS2 and AS3, isolated from the sewage and pond waters of the outskirts of Kolkata. A few phages, named AS4, with hexagonal heads and abnormally long tails with typical curly projections were also found in the water samples. ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-2-28

    authors: Sen A,Ghosh AN

    更新日期:2005-04-11 00:00:00

  • Vaccination of ferrets with a recombinant G glycoprotein subunit vaccine provides protection against Nipah virus disease for over 12 months.

    abstract:BACKGROUND:Nipah virus (NiV) is a zoonotic virus belonging to the henipavirus genus in the family Paramyxoviridae. Since NiV was first identified in 1999, outbreaks have continued to occur in humans in Bangladesh and India on an almost annual basis with case fatality rates reported between 40% and 100%. METHODS:Ferret...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-237

    authors: Pallister JA,Klein R,Arkinstall R,Haining J,Long F,White JR,Payne J,Feng YR,Wang LF,Broder CC,Middleton D

    更新日期:2013-07-16 00:00:00

  • A recombinant adenovirus-based vector elicits a specific humoral immune response against the V3 loop of HIV-1 gp120 in mice through the "Antigen Capsid-Incorporation" strategy.

    abstract:BACKGROUND:Due to potential advantages, human adenoviral vectors have been evaluated pre-clinically as recombinant vaccine vectors against several cancers and infectious diseases, including human immunodeficiency virus (HIV) infection. The V3 loop of HIV-1 glycoprotein 120 (gp120) contains important neutralizing epitop...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-112

    authors: Gu L,Krendelchtchikova V,Krendelchtchikov A,Oster RA,Fujihashi K,Matthews QL

    更新日期:2014-06-16 00:00:00

  • Chinese highly pathogenic porcine reproductive and respiratory syndrome virus exhibits more extensive tissue tropism for pigs.

    abstract:BACKGROUND:The highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) emerging in China exhibits high fatality to pigs. However, the mechanism related to the increased pathogenicity of the virus remains unclear. In the present study, the differences in tissue tropism between the highly pathogenic...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-203

    authors: Li L,Zhao Q,Ge X,Teng K,Kuang Y,Chen Y,Guo X,Yang H

    更新日期:2012-09-17 00:00:00

  • Viral trans-factor independent replication of human papillomavirus genomes.

    abstract:BACKGROUND:Papillomaviruses (PVs) establish a persistent infection in the proliferating basal cells of the epithelium. The viral genome is replicated and maintained as a low-copy nuclear plasmid in basal keratinocytes. Bovine and human papillomaviruses (BPV and HPV) are known to utilize two viral proteins; E1, a DNA he...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-123

    authors: Pittayakhajonwut D,Angeletti PC

    更新日期:2010-06-10 00:00:00

  • Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

    abstract:BACKGROUND:Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread. METHODS:The study population consis...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-014-0194-z

    authors: Grgić H,Costa M,Friendship RM,Carman S,Nagy É,Wideman G,Weese S,Poljak Z

    更新日期:2014-11-22 00:00:00

  • Epidemics of viral meningitis caused by echovirus 6 and 30 in Korea in 2008.

    abstract:BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-38

    authors: Kim HJ,Kang B,Hwang S,Hong J,Kim K,Cheon DS

    更新日期:2012-02-15 00:00:00

  • Cellular transcripts of chicken brain tissues in response to H5N1 and Newcastle disease virus infection.

    abstract:BACKGROUND:Highly-pathogenic avian influenza (HPAI) H5N1 and Newcastle disease (ND) viruses are the two most important poultry viruses in the world, with the ability to cause classic central nervous system dysfunction in poultry and migratory birds. To elucidate the mechanisms of neurovirulence caused by these viruses,...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-53

    authors: Balasubramaniam VR,Wai TH,Omar AR,Othman I,Hassan SS

    更新日期:2012-02-23 00:00:00

  • Genome characterization of Long Island tick rhabdovirus, a new virus identified in Amblyomma americanum ticks.

    abstract:BACKGROUND:Ticks are implicated as hosts to a wide range of animal and human pathogens. The full range of microbes harbored by ticks has not yet been fully explored. METHODS:As part of a viral surveillance and discovery project in arthropods, we used unbiased high-throughput sequencing to examine viromes of ticks coll...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-26

    authors: Tokarz R,Sameroff S,Leon MS,Jain K,Lipkin WI

    更新日期:2014-02-11 00:00:00

  • Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge.

    abstract:BACKGROUND:Porcine epidemic diarrhea virus (PEDV) is the main causative agent of porcine epidemic diarrhea (PED). Since December 2010, a large-scale outbreak of diarrhea has been observed in swine farms in China. Accumulated evidence indicates that this large-scale outbreak of diarrhea were caused by highly virulent PE...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0529-z

    authors: Lin H,Chen L,Gao L,Yuan X,Ma Z,Fan H

    更新日期:2016-04-22 00:00:00

  • Evaluation of nanoparticle tracking analysis for total virus particle determination.

    abstract::The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method's accuracy was demonstrated by using known...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-265

    authors: Kramberger P,Ciringer M,Štrancar A,Peterka M

    更新日期:2012-11-12 00:00:00

  • The full genome sequence of three strains of Jamestown Canyon virus and their pathogenesis in mice or monkeys.

    abstract:BACKGROUND:Jamestown Canyon virus (JCV), family Bunyaviridae, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of t...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-136

    authors: Bennett RS,Nelson JT,Gresko AK,Murphy BR,Whitehead SS

    更新日期:2011-03-24 00:00:00

  • Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

    abstract:BACKGROUND:Fast, reliable and easy to handle methods are required to facilitate urgently needed point-of-care testing (POCT) in the current coronavirus pandemic. Life-threatening severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread all over the world, infecting more than 33,500,000 people and...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01435-6

    authors: Mautner L,Baillie CK,Herold HM,Volkwein W,Guertler P,Eberle U,Ackermann N,Sing A,Pavlovic M,Goerlich O,Busch U,Wassill L,Huber I,Baiker A

    更新日期:2020-10-21 00:00:00

  • Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.

    abstract:BACKGROUND:The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. METHODS:Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. RESULTS:Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0701-0

    authors: Cui Z,Feng T,Gu F,Li Q,Dong K,Zhang Y,Zhu Y,Han L,Qin J,Guo X

    更新日期:2017-02-08 00:00:00

  • Local production of CCL3, CCL11, and IFN-γ correlates with disease severity in murine parainfluenza virus infection.

    abstract:BACKGROUND:Using a murine model of parainfluenza virus infection (mPIV1 or Sendai virus; SeV), we compared the inflammatory responses to lethal and sub-lethal infections in inbred DBA/2 mice. METHODS:Mice were intranasally inoculated with either 1.6×10(3) or 1.6×10(5) infectious units (IU) of SeV or diluent control. C...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-357

    authors: Suryadevara M,Bonville CA,Rosenberg HF,Domachowske JB

    更新日期:2013-12-21 00:00:00

  • Acute flaccid myelitis associated with enterovirus-D68 infection in an otherwise healthy child.

    abstract:BACKGROUND:Reporting new cases of enterovirus (EV)-D68-associated acute flaccid myelitis (AFM) is essential to understand how the virus causes neurological damage and to characterize EV-D68 strains associated with AFM. CASE PRESENTATION:A previously healthy 4-year-old boy presented with sudden weakness and limited mob...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0678-0

    authors: Esposito S,Chidini G,Cinnante C,Napolitano L,Giannini A,Terranova L,Niesters H,Principi N,Calderini E

    更新日期:2017-01-11 00:00:00

  • Naturally occurring mutations to HCV protease inhibitors in treatment-naïve patients.

    abstract:BACKGROUND:Protease inhibitors (PIs) to treat hepatitis C (HCV) virus infection have been approved and others are under development. RESULTS:The aims of this study were to illustrate natural polymorphisms in the HCV protease and measure the frequency of PI resistance mutations in different HCV genotypes from PI-naïve ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-245

    authors: Paolucci S,Fiorina L,Piralla A,Gulminetti R,Novati S,Barbarini G,Sacchi P,Gatti M,Dossena L,Baldanti F

    更新日期:2012-10-24 00:00:00

  • Infectious pancreatic necrosis virus (IPNV) from salmonid fish enters, but does not replicate in, mammalian cells.

    abstract:BACKGROUND:The aquatic birnavirus infectious pancreatic necrosis virus (IPNV) causes infectious pancreatic necrosis (IPN), a severe disease in farmed salmonid fish. IPNV has a very broad host range and infects many different species of fish as well as molluscs and crustaceans. Investigation of the host reservoir of a v...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-228

    authors: Ørpetveit I,Küntziger T,Sindre H,Rimstad E,Dannevig BH

    更新日期:2012-10-05 00:00:00

  • Over-expression of mitochondrial antiviral signaling protein inhibits coxsackievirus B3 infection by enhancing type-I interferons production.

    abstract:BACKGROUND:Recent studies have revealed that Mitochondrial Antiviral Signaling (MAVS) protein plays an essential role in the inhibition of viral infection through type I interferon (IFN) pathway. It has been shown that 3C (pro) cysteine protease of coxsackievirus B3 (CVB3) cleaves MAVS to inhibit type I IFNs induction....

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-312

    authors: Zhang QM,Song WQ,Li YJ,Qian J,Zhai AX,Wu J,Li AM,He JM,Zhao JY,Yu X,Wei LL,Zhang FM

    更新日期:2012-12-19 00:00:00

  • Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo.

    abstract::During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-le...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-131

    authors: Pfefferle S,Krähling V,Ditt V,Grywna K,Mühlberger E,Drosten C

    更新日期:2009-08-24 00:00:00

  • Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression.

    abstract:BACKGROUND:Bovine leukemia virus (BLV), which is closely related to human T-cell leukemia virus, is the etiological agent of enzootic bovine leukosis, a disease characterized by a highly prolonged course involving persistent lymphocytosis and B-cell lymphoma. The bovine major histocompatibility complex class II region ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-019-1259-9

    authors: Bai L,Takeshima SN,Sato M,Davis WC,Wada S,Kohara J,Aida Y

    更新日期:2019-12-16 00:00:00

  • Drug susceptibility to etravirine and darunavir among Human Immunodeficiency Virus Type 1-derived pseudoviruses in treatment-experienced patients with HIV/AIDS in South Korea.

    abstract:BACKGROUND:In South Korea, about 20 types of antiretroviral drugs are used in the treatment of patients with human immunodeficiency virus/acquired immune deficiency syndrome. Since 2010, raltegravir, etravirine, and darunavir have been spotlighted as new drugs for highly active antiretroviral therapy (HAART)-experience...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0283-7

    authors: Kwon OK,Kim SS,Rhee JE,Kee MK,Park M,Oh HR,Choi JY

    更新日期:2015-04-09 00:00:00

  • HHV-6A in vitro infection of thyrocytes and T cells alters the expression of miRNA associated to autoimmune thyroiditis.

    abstract:BACKGROUND:Human herpesviruses have been hypothesized as environmental triggers in the development of autoimmune thyroid diseases (AITD), and in particular active human herpesvirus 6A (HHV-6A) infection was detected in thyrocytes of Hashimoto's thyroiditis (HT) patients, who also show specific anti-viral immune respons...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0672-6

    authors: Caselli E,D'Accolti M,Soffritti I,Zatelli MC,Rossi R,Degli Uberti E,Di Luca D

    更新日期:2017-01-11 00:00:00

  • Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India: evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains.

    abstract:BACKGROUND:During the pandemic [Influenza A(H1N1)pdm09] period in 2009-2010, an influenza A (Inf-A) virus with H1N2 subtype (designated as A/Eastern India/N-1289/2009) was detected from a 25 years old male from Mizoram (North-eastern India). OBJECTIVE:To characterize full genome of the H1N2 influenza virus. METHODS:F...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-233

    authors: Mukherjee TR,Agrawal AS,Chakrabarti S,Chawla-Sarkar M

    更新日期:2012-10-11 00:00:00

  • Full-length Ebola glycoprotein accumulates in the endoplasmic reticulum.

    abstract::The Filoviridae family comprises of Ebola and Marburg viruses, which are known to cause lethal hemorrhagic fever. However, there is no effective anti-viral therapy or licensed vaccines currently available for these human pathogens. The envelope glycoprotein (GP) of Ebola virus, which mediates entry into target cells, ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-11

    authors: Bhattacharyya S,Hope TJ

    更新日期:2011-01-12 00:00:00