当前位置： SCI文献检索 > Virology Journal期刊下所有文献 > A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus.

A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus.

Abstract：

BACKGROUND:Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be required when HR fails. Previously, in vitro ligation has been suggested to facilitate construction of E1/E3-deleted, replication-incompetent Ad vectors. However, in vitro ligation has only rarely been used to generate CRAds and may be a complex procedure for molecular biologists who are not experts in the field. METHODS AND RESULTS:A modified in vitro ligation approach was developed to construct a double-targeted, E1B55k-deleted CRAd. The method allowed the incorporation of a tumor-specific promoter, e.g. the heat-shock protein 70 (hsp70) promoter, upstream of E1a, deletion of the E1B55k gene, and HR-free cloning of the recombined E1Delta55k gene into the Ad genome. The genetic structure of the CRAd was confirmed using restriction analysis and PCR. The replication rate of the hsp70E1Delta55k CRAd was 1.5-2% of Ad without E1Delta55k deletion. CONCLUSION:A 3-step cloning approach can generate a double-targeted, E1B55k-deleted CRAd using a straight-forward, modified in vitro ligation procedure.

journal_name

Virol J

journal_title

Virology journal

authors

Haviv YS

doi

10.1186/1743-422X-6-18

subject

Has Abstract

pub_date

2009-02-07 00:00:00

pages

18

issn

1743-422X

pii

1743-422X-6-18

journal_volume

6

pub_type

杂志文章

相关文献

Virology Journal文献大全
  • Over-expression of mitochondrial antiviral signaling protein inhibits coxsackievirus B3 infection by enhancing type-I interferons production.

    abstract：BACKGROUND:Recent studies have revealed that Mitochondrial Antiviral Signaling (MAVS) protein plays an essential role in the inhibition of viral infection through type I interferon (IFN) pathway. It has been shown that 3C (pro) cysteine protease of coxsackievirus B3 (CVB3) cleaves MAVS to inhibit type I IFNs induction....

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-312

    authors： Zhang QM,Song WQ,Li YJ,Qian J,Zhai AX,Wu J,Li AM,He JM,Zhao JY,Yu X,Wei LL,Zhang FM

    更新日期：2012-12-19 00:00:00

  • Packaging of viral RNAs in virions of adenoviruses.

    abstract：:Earlier, we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than empty/intermediate capsids. Some of the packaged viral RNAs appear t...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-6-16

    authors： Xing L,Tikoo SK

    更新日期：2009-02-05 00:00:00

  • Newcastle disease virus-vectored West Nile fever vaccine is immunogenic in mammals and poultry.

    abstract：BACKGROUND:West Nile virus (WNV) is an emerging zoonotic pathogen which is harmful to human and animal health. Effective vaccination in susceptible hosts should protect against WNV infection and significantly reduce viral transmission between animals and from animals to humans. A versatile vaccine suitable for differen...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-016-0568-5

    authors： Wang J,Yang J,Ge J,Hua R,Liu R,Li X,Wang X,Shao Y,Sun E,Wu D,Qin C,Wen Z,Bu Z

    更新日期：2016-06-24 00:00:00

  • Human immunodeficiency virus type 1 ((HIV-1) subtypes in the northwest region, Cameroon.

    abstract：BACKGROUND:The high genetic diversity of HIV-1 has been shown to influence the global distribution, disease progression, treatment success, and the development of an effective vaccine. Despite the low HIV prevalence in Cameroon, all the major HIV subtypes alongside several circulating recombinant forms (CRFs) and uniqu...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-019-1209-6

    authors： Abongwa LE,Nyamache AK,Torimiro JN,Okemo P,Charles F

    更新日期：2019-08-15 00:00:00

  • Posttranslational N-glycosylation of the hepatitis B virus large envelope protein.

    abstract：BACKGROUND:The addition of N-linked glycans to proteins is normally a cotranslational process that occurs during translocation of the nascent protein to the endoplasmic reticulum. Here, we report on an exception to this rule occurring on the hepatitis B virus (HBV) large L envelope protein that is a subject to co-plus ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-4-45

    authors： Lambert C,Prange R

    更新日期：2007-05-30 00:00:00

  • Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0.

    abstract：BACKGROUND:Herpes simplex viruses (HSVs) rapidly shut off macromolecular synthesis in host cells. In contrast, global microarray analyses have shown that HSV infection markedly up-regulates a number of host cell genes that may play important roles in HSV-host cell interactions. To understand the regulatory mechanisms i...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-257

    authors： Kamakura M,Goshima F,Luo C,Kimura H,Nishiyama Y

    更新日期：2011-05-25 00:00:00

  • Origin of measles virus: divergence from rinderpest virus between the 11th and 12th centuries.

    abstract：:Measles, caused by measles virus (MeV), is a common infection in children. MeV is a member of the genus Morbillivirus and is most closely related to rinderpest virus (RPV), which is a pathogen of cattle. MeV is thought to have evolved in an environment where cattle and humans lived in close proximity. Understanding th...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-52

    authors： Furuse Y,Suzuki A,Oshitani H

    更新日期：2010-03-04 00:00:00

  • Characterization of neuraminidase inhibitor-resistant influenza virus isolates from immunocompromised patients in the Republic of Korea.

    abstract：BACKGROUND:The emergence of influenza viruses resistant to anti-influenza drugs is a threat to global public health. The Korea Centers for Disease Control and Prevention operates the Korea Influenza and Respiratory Viruses Surveillance System (KINRESS) to monitor epidemics of influenza and Severe Acute Respiratory Infe...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-020-01375-1

    authors： Kim HM,Lee N,Kim MS,Kang C,Chung YS

    更新日期：2020-07-06 00:00:00

  • Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro.

    abstract：BACKGROUND:Human adenoviruses (Ads) have substantial potential for clinical applications in cancer patients. Conditionally replicating adenoviruses (CRAds) include oncolytic adenoviruses in which expression of the immediate early viral transactivator protein E1A is controlled by a cancer cell-selective promoter. To enh...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-175

    authors： Fontecedro AC,Lutschg V,Eichhoff O,Dummer R,Greber UF,Hemmi S

    更新日期：2010-07-29 00:00:00

  • Epidemics of viral meningitis caused by echovirus 6 and 30 in Korea in 2008.

    abstract：BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-38

    authors： Kim HJ,Kang B,Hwang S,Hong J,Kim K,Cheon DS

    更新日期：2012-02-15 00:00:00

  • Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States.

    abstract：BACKGROUND:Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry. However, the virome of PEDV infected pigs and its effect on disease severity remains unknown. The advancements of sequencing technology have mad...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-018-1001-z

    authors： Chen Q,Wang L,Zheng Y,Zhang J,Guo B,Yoon KJ,Gauger PC,Harmon KM,Main RG,Li G

    更新日期：2018-05-25 00:00:00

  • Nuclear localized Influenza nucleoprotein N-terminal deletion mutant is deficient in functional vRNP formation.

    abstract：BACKGROUND:The influenza RNA dependent RNA polymerase synthesizes viral RNA in the nucleus as functional viral ribonucleoprotein (vRNP) complexes with RNA and nucleoprotein (NP). The N-terminus of NP contains an unconventional nuclear localization signal (NLS) important for initial vRNP nuclear localization but which a...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-11-155

    authors： Sanchez A,Guerrero-Juarez CF,Ramirez J,Newcomb LL

    更新日期：2014-08-31 00:00:00

  • Comparative efficacy of experimental inactivated and live-attenuated chimeric porcine circovirus (PCV) 1-2b vaccines derived from PCV1 and PCV2b isolates originated in China.

    abstract：BACKGROUND:Porcine circovirus type-2b (PCV2b) is recognized as the etiological agent of the various clinical manifestations of porcine circovirus-associated disease (PCVAD). Previous studies have demonstrated effectiveness of chimeric PCV1-2 vaccines against PCV2b challenge. In this study, the efficacy of inactivated a...

    journal_title：Virology journal

    pub_type： 杂志文章,随机对照试验

    doi：10.1186/s12985-015-0338-9

    authors： Li J,Yu T,Wang X,Zhou J,Gao R,Zhang F,Gao X,Gao S,Liu X

    更新日期：2015-07-30 00:00:00

  • Seroprevalence of human respiratory syncytial virus and human metapneumovirus in healthy population analyzed by recombinant fusion protein-based enzyme linked immunosorbent assay.

    abstract：BACKGROUND:Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are two of the most frequent respiratory pathogens that circulate worldwide. Infection with either virus can lead to hospitalization of young children, immunocompromised people and the elderly.A better understanding of the epidemiologi...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-9-130

    authors： Sastre P,Ruiz T,Schildgen O,Schildgen V,Vela C,Rueda P

    更新日期：2012-07-02 00:00:00

  • Evidence of recombination within human alpha-papillomavirus.

    abstract：BACKGROUND:Human papillomavirus (HPV) has a causal role in cervical cancer with almost half a million new cases occurring each year. Presence of the carcinogenic HPV is necessary for the development of the invasive carcinoma of the genital tract. Therefore, persistent infection with carcinogenic HPV causes virtually al...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-4-33

    authors： Angulo M,Carvajal-Rodríguez A

    更新日期：2007-03-28 00:00:00

  • An antigenic epitope of influenza virus nucleoprotein (NP) associated with polymeric forms of NP.

    abstract：:Intracellular influenza virus nucleoprotein (NP) is characterized by a high efficiency of homo-polymers formation, however their antigenic structure is still incompletely known. Herein, we report that RNase-resistant intracellular NP homo-polymers have a highly ordered conformational antigenic epitope, which depends o...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-5-37

    authors： Prokudina EN,Semenova N,Chumakov V,Stitz L

    更新日期：2008-02-29 00:00:00

  • Evidence of recombination in quasispecies populations of a Hepatitis C Virus patient undergoing anti-viral therapy.

    abstract：BACKGROUND/AIM:Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease has emerged. HCV circulates in vivo as a complex population of different but closely related viral variants, commonly referred to as a quasispecies. The extent to which recombina...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-3-87

    authors： Moreno MP,Casane D,López L,Cristina J

    更新日期：2006-10-24 00:00:00

  • Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone.

    abstract：BACKGROUND:Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to prevent chronic infection is important. We reported earlier that a re...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-118

    authors： Chandra PK,Hazari S,Poat B,Gunduz F,Prabhu R,Liu G,Burioni R,Clementi M,Garry RF,Dash S

    更新日期：2010-06-07 00:00:00

  • Replicative homeostasis III: implications for antiviral therapy and mechanisms of response and non-response.

    abstract：:While improved drug regimens have greatly enhanced outcomes for patients with chronic viral infection, antiviral therapy is still not ideal due to drug toxicities, treatment costs, primary drug failure and emergent resistance. New antiviral agents, alternative treatment strategies and a better understanding of viral p...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-4-29

    authors： Sallie R

    更新日期：2007-03-13 00:00:00

  • Molecular approaches to the analysis of deformed wing virus replication and pathogenesis in the honey bee, Apis mellifera.

    abstract：BACKGROUND:For years, the understanding of the pathogenetic mechanisms that underlie honey bee viral diseases has been severely hindered because of the lack of a cell culture system for virus propagation. As a result, it is very imperative to develop new methods that would permit the in vitro pathogenesis study of hone...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-6-221

    authors： Boncristiani HF Jr,Di Prisco G,Pettis JS,Hamilton M,Chen YP

    更新日期：2009-12-11 00:00:00

  • A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus.

    abstract：BACKGROUND:Respiratory syncytial virus (RSV), human Rhinovirus (HRV) and human Metapneumo Virus (HMPV) are important viral pathogens causing acute respiratory tract infections in the hospitalized patients. Sensitive and accurate detection of RSV, HRV and HMPV is necessary for clinical diagnosis and treatment. RESULTS:...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-018-1061-0

    authors： Feng ZS,Zhao L,Wang J,Qiu FZ,Zhao MC,Wang L,Duan SX,Zhang RQ,Chen C,Qi JJ,Fan T,Li GX,Ma XJ

    更新日期：2018-10-30 00:00:00

  • Potential effects of telbivudine and entecavir on renal function: a systematic review and meta-analysis.

    abstract：BACKGROUND:To assess the potential effects of telbivudine (LdT) and entecavir (ETV) on renal function in patients with chronic hepatitis B (CHB), we performed a meta-analysis of the relevant data available on these agents to evaluate their effects on the estimated glomerular filtration rate (eGFR) during treatment. ME...

    journal_title：Virology journal

    pub_type： 杂志文章,meta分析,评审

    doi：10.1186/s12985-016-0522-6

    authors： Wu X,Cai S,Li Z,Zheng C,Xue X,Zeng J,Peng J

    更新日期：2016-04-09 00:00:00

  • Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray.

    abstract：BACKGROUND:The Epstein-Barr virus (EBV) is associated with lymphoid malignancies, including Burkitt's lymphoma (BL), and can transform human B cells in vitro. EBV-harboring cell lines are widely used to investigate lymphocyte transformation and oncogenesis. Qualitative EBV gene expression has been extensively described...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-3-43

    authors： Bernasconi M,Berger C,Sigrist JA,Bonanomi A,Sobek J,Niggli FK,Nadal D

    更新日期：2006-06-06 00:00:00

  • Crystal structure of the fibre head domain of bovine adenovirus 4, a ruminant atadenovirus.

    abstract：BACKGROUND:In adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other adenovirus capsid proteins and leads to a productive infection. Altho...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-015-0309-1

    authors： Nguyen TH,Vidovszky MZ,Ballmann MZ,Sanz-Gaitero M,Singh AK,Harrach B,Benkő M,van Raaij MJ

    更新日期：2015-05-22 00:00:00

  • Standardized positive controls for detection of norovirus by reverse transcription PCR.

    abstract：BACKGROUND:Norovirus is one of the most common causes of nonbacterial gastroenteritis in humans. Rapid spread by contaminated food and person-to-person transmission through the fecal-oral route are characteristics of norovirus epidemiology and result in high morbidity in vulnerable patient populations. Therefore, detec...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-260

    authors： Lee SG,Lee SH,Park SW,Suh CI,Jheong WH,Oh S,Paik SY

    更新日期：2011-05-26 00:00:00

  • A novel protein-coding ORF72.2 gene was identified from Marek's disease virus strain CVI988.

    abstract：:Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing o...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-7-371

    authors： Tian M,Zhao Y,Shi M,Lin Y,Zou N,Liu P,Wen X,Cao S,Huang Y

    更新日期：2010-12-23 00:00:00

  • Vector competence of Aedes aegypti in transmitting Chikungunya virus: effects and implications of extrinsic incubation temperature on dissemination and infection rates.

    abstract：BACKGROUND:Aedes aegypti is a competent arthropod vector of chikungunya virus (CHIKV). The rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. This stu...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-016-0566-7

    authors： Mbaika S,Lutomiah J,Chepkorir E,Mulwa F,Khayeka-Wandabwa C,Tigoi C,Oyoo-Okoth E,Mutisya J,Ng'ang'a Z,Sang R

    更新日期：2016-06-29 00:00:00

  • Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice.

    abstract：BACKGROUND:The incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for dengue fever, and licensed vaccine against dengue is not available. ...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/1743-422X-8-333

    authors： Zhang S,Liang M,Gu W,Li C,Miao F,Wang X,Jin C,Zhang L,Zhang F,Zhang Q,Jiang L,Li M,Li D

    更新日期：2011-06-30 00:00:00

  • Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case.

    abstract：BACKGROUND:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS:We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, k...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-018-1006-7

    authors： Li S,Li Y,Wang Q,Yu X,Liu M,Xie H,Qian L,Ye L,Yang Z,Zhang J,Zhu H,Zhang W

    更新日期：2018-05-30 00:00:00

  • Chikungunya virus infection in Cynomolgus macaques following Intradermal and aerosol exposure.

    abstract：BACKGROUND:Chikungunya virus (CHIKV) is transmitted via mosquito bite and potentially by aerosol, causing chikungunya fever and arthritic disease in humans. There are currently no licensed vaccines or antiviral therapeutics to protect against CHIKV infection in humans. Animal models recapitulating human disease, especi...

    journal_title：Virology journal

    pub_type： 杂志文章

    doi：10.1186/s12985-017-0804-7

    authors： Cirimotich CM,Vela EM,Garver J,Barnewall RE,Miller BD,Meister GT,Rogers JV

    更新日期：2017-07-20 00:00:00