Abstract:
BACKGROUND:Respiratory syncytial virus (RSV), human Rhinovirus (HRV) and human Metapneumo Virus (HMPV) are important viral pathogens causing acute respiratory tract infections in the hospitalized patients. Sensitive and accurate detection of RSV, HRV and HMPV is necessary for clinical diagnosis and treatment. RESULTS:A locked nucleic acid (LNA)-based multiplex closed one-tube nested real-time RT-PCR (mOTNRT-PCR) assay was developed for simultaneous detection of RSV, HRV and HMPV. The sensitivity, specificity, reproducibility and clinical performance of mOTNRT-PCR were evaluated and compared with individual real time PCR (RT-qPCR) assay using clinical samples. The analytical sensitivity of mOTNRT-PCR assay was 5 copies/reaction for RSV, HRV and HMPV, respectively, and no cross-reaction with other common respiratory viruses was observed. The coefficients of variation (CV) of intra-assay and inter-assay were between 0.51 to 3.67%. Of 398 nasopharyngeal aspirates samples tested, 109 (27.39%), 150 (37.69%) and 44 (11.06%) were positive for RSV, HRV and HMPV, respectively, whereas 95 (23.87%), 137 (34.42%) and 38 (9.55%) were positive for RSV, HRV and HMPV, respectively, by individual RT-qPCR assay. Thirty three samples that were positive by mOTNRT-PCR but negative by RT-qPCR were confirmed as true positives by sequencing using reported traditional two-step nested PCR assay. CONCLUSION:mOTNRT-PCR assay reveals extremely higher sensitivity than that of RT-qPCR assay for detecting RSV, HRV and HMPV in clinical settings.
journal_name
Virol Jjournal_title
Virology journalauthors
Feng ZS,Zhao L,Wang J,Qiu FZ,Zhao MC,Wang L,Duan SX,Zhang RQ,Chen C,Qi JJ,Fan T,Li GX,Ma XJdoi
10.1186/s12985-018-1061-0subject
Has Abstractpub_date
2018-10-30 00:00:00pages
167issue
1issn
1743-422Xpii
10.1186/s12985-018-1061-0journal_volume
15pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Cervical cancer is a major public health problem worldwide. While Romania has the highest incidence of cervical cancer in Europe, the prevalence of HPV has not been evaluated. We report the first data on HPV prevalence and type distribution in Northeast Romania. METHODS:HPV prevalence and genotype distribut...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-558
更新日期:2011-12-22 00:00:00
abstract::Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, w...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-42
更新日期:2008-03-19 00:00:00
abstract:OBJECTIVE:To determine HIV-1 RNA load during the third trimester of pregnancy and evaluate its effect on in utero and intra-partum/postpartum transmissions in a breastfeeding population. DESIGN:A nested case-control study within a PMTCT cohort of antiretroviral therapy naive pregnant women and their infants. METHODS:...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-176
更新日期:2010-08-02 00:00:00
abstract:BACKGROUND:Hepatitis C Virus (HCV) infected patients are frequently repeatedly exposed to the virus, but very few recombinants between two genotypes have been reported. FINDINGS:We describe the discovery of an HCV recombinant using a method developed in a United States clinical lab for HCV genotyping that employs sequ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-458
更新日期:2011-10-03 00:00:00
abstract:BACKGROUND:Finding new biomarkers for the early detection of cervical cancer is an essential requirement in this field. In this study, we aimed to evaluate the expression level of potential biomarkers in progression of cervical cancer in patients with cervical cancer compared to normal subjects. METHODS:The expression...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1220-y
更新日期:2019-10-07 00:00:00
abstract:BACKGROUND:Protease inhibitors (PIs) to treat hepatitis C (HCV) virus infection have been approved and others are under development. RESULTS:The aims of this study were to illustrate natural polymorphisms in the HCV protease and measure the frequency of PI resistance mutations in different HCV genotypes from PI-naïve ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-245
更新日期:2012-10-24 00:00:00
abstract:BACKGROUND:The Unique Long 26 (UL26) and UL26.5 proteins of herpes simplex virus are known to function during the assembly of the viruses. However, for duck enteritis virus (DEV), which is an unassigned member of the family Herpesviridae, little information is available about the function of the two proteins. In this s...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-223
更新日期:2010-09-13 00:00:00
abstract:BACKGROUND:The COVID-19 causing coronavirus is an enveloped RNA virus that utilizes an enzyme RNA dependent RNA polymerase for its replication. Favipiravir (FVP) triphosphate, a purine nucleoside analog, inhibits that enzyme. We have conducted this systematic review and meta-analysis on efficacy and safety of the drug ...
journal_title:Virology journal
pub_type: 杂志文章,meta分析
doi:10.1186/s12985-020-01412-z
更新日期:2020-09-24 00:00:00
abstract:BACKGROUND:During the last two decades, structural biology analyses have shown that viruses infecting hosts far apart in evolution share similar architectural features, prompting a new virus classification based on structural lineages. Until recently, only a few prokaryotic viruses had been described for one of the lin...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-018-1097-1
更新日期:2018-11-23 00:00:00
abstract:BACKGROUND:Hepatitis C virus roots a chronic liver disease. Currently approved treatment strategy includes administration of alpha interferon and ribavirin combined therapy for 24-48 weeks. One of the predictor of sustained virological response is an early virological response to treatment characterized as rapid respon...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-253
更新日期:2011-05-23 00:00:00
abstract:BACKGROUND:PCR-based detection and identification of viruses assumes a known, relatively stable genome. Unfortunately, high mutation rates may lead to extensive changes in viral nucleic acid sequences making dedicated PCR primer use problematic. Furthermore, in bioterrorism, viral consensus sequences can be genetically...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-65
更新日期:2007-06-28 00:00:00
abstract:BACKGROUND:A vaccine against all four dengue virus (DENV) serotypes includes the formulation of one genotype of each serotype. Although genetic similarities among genotypes within a serotype are higher as compared to those among serotypes, differences in the immunogenicity of the included genotypes would be a critical ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-0967-x
更新日期:2018-03-27 00:00:00
abstract:BACKGROUND:The epidemic pattern of respiratory syncytial virus (RSV) is quite different in regions of Europe (biennial epidemics in alternating cycles of approximately 9 and 15 months) than in the Western Hemisphere (annual epidemics). In order to determine if these differences are accounted for by the circulation of d...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-133
更新日期:2009-09-07 00:00:00
abstract:: Previously, we explored the epidemic pattern and molecular characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006. The present study extended these observations to 2008 and 2009. In this study, enteroviruses showed similar seasonal prevalent pattern from summer to fall and age distribution to...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-297
更新日期:2011-06-13 00:00:00
abstract::Hepatitis C virus (HCV) is endemic worldwide, and it causes cirrhosis and other complications that often lead to death; nevertheless, our knowledge of the disease and its mechanisms is limited. HCV is most common in underdeveloped nations, including many in Africa and Asia. The virus is usually transmitted by parenter...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-9-57
更新日期:2012-03-02 00:00:00
abstract::Earlier, we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than empty/intermediate capsids. Some of the packaged viral RNAs appear t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-16
更新日期:2009-02-05 00:00:00
abstract:BACKGROUND:Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it. METHOD:In this study, we attempted to eluci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0654-8
更新日期:2016-11-25 00:00:00
abstract:BACKGROUND:Human cytomegalovirus (HCMV) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. HCMV immediate early (IE) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (ORF) UL82, and this transactivation activity...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-65
更新日期:2009-05-27 00:00:00
abstract::Herpes simplex virus (HSV) infection is one of the most common viral sexually transmitted diseases worldwide. The first time infection of the mother may lead to severe illness in pregnancy and may be associated with virus transmission from mother to foetus/newborn. Since the incidence of this sexually transmitted infe...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-6-40
更新日期:2009-04-06 00:00:00
abstract:BACKGROUND:The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic p...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-44
更新日期:2012-02-16 00:00:00
abstract:BACKGROUND:The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive samplin...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01298-x
更新日期:2020-03-05 00:00:00
abstract:BACKGROUND:Chikungunya virus (CHIKV) is transmitted via mosquito bite and potentially by aerosol, causing chikungunya fever and arthritic disease in humans. There are currently no licensed vaccines or antiviral therapeutics to protect against CHIKV infection in humans. Animal models recapitulating human disease, especi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0804-7
更新日期:2017-07-20 00:00:00
abstract:BACKGROUND:The Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT (of HN-A1081 viral population) and VGly (of HN-G1081). The V protein is a promoting factor of viral replication by blocking the IFN antiviral pathway. FINDINGS:We studied the relationship between V protein variants and IFN-α...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-224
更新日期:2011-05-13 00:00:00
abstract:BACKGROUND:Lymphocytes provide invaluable whistle blowers of changes due to infections. We use the information registered by these cells using their mRNAs as they encounter the pathogen to develop patterns of expression that correspond to that specific pathogen. Venezuelan equine encephalitis (VEE) is a mosquito-borne ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-82
更新日期:2007-08-29 00:00:00
abstract:AIM:To investigate the enhancement of humoral immunity when CpG ODN (cytidine phosphate guanosine oligodeoxynucleotides) and aluminium adjuvants are complexed with the HCV (Hepatitis C virus) recombinant immunogen in mice. METHODS:After immunizing Balb/c mice with the recombination HCV antigen adjuvanted with pUCpGs10...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-507
更新日期:2011-11-04 00:00:00
abstract:BACKGROUND:Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and sub-tropical countries, affecting about 50 million people around the world yearly. In Colombia, dengue virus was first detected in 1971 and still remains as a major public health issue. Although four viral serotyp...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-64
更新日期:2012-03-09 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV) infection is a serious health problem worldwide. Treatment recommendation and response are mainly indicated by viral load, e antigen (HBeAg) seroconversion, and ALT levels. The S antigen (HBsAg) seroconversion is much less frequent. Since HBeAg can be negative in the presence of high ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-93
更新日期:2007-09-24 00:00:00
abstract::Much of the worlds' population is in active or imminent danger from established infectious pathogens, while sporadic and pandemic infections by these and emerging agents threaten everyone. RNA polymerases (RNApol) generate enormous genetic and consequent antigenic heterogeneity permitting both viruses and cellular pat...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-2-70
更新日期:2005-08-22 00:00:00
abstract:BACKGROUND:Cytomegalovirus (CMV) congenital infection is the major viral cause of well-documented birth defects in human. Because CMV is species-specific, the main obstacle to developing animal models for congenital infection is the difference in placental architecture, which preludes virus transmission across the plac...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-42
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Avian hepatitis E virus (aHEV) has been associated with hepatitis-splenomegaly syndrome (HSS) in chickens along with asymptomatic subclinical infection in many cases. So far, four genotypes have been described, which cause infection in chickens, specifically in broiler breeders and layer chickens. In the pre...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1247-0
更新日期:2019-11-21 00:00:00