Chikungunya virus infection in Cynomolgus macaques following Intradermal and aerosol exposure.

Abstract:

BACKGROUND:Chikungunya virus (CHIKV) is transmitted via mosquito bite and potentially by aerosol, causing chikungunya fever and arthritic disease in humans. There are currently no licensed vaccines or antiviral therapeutics to protect against CHIKV infection in humans. Animal models recapitulating human disease, especially for transmission by aerosol, are needed for licensure of such medical countermeasures. METHODS:Cynomolgus macaques (CMs) were challenged by intradermal (ID) inoculation or exposure to an aerosol containing CHIKV Ross strain at different target infectious doses (103-107 plaque forming units (PFU)). The clinical and virologic courses of disease were monitored up to 14 days post-exposure. RESULTS:ID infection of CMs led to overt clinical disease, detectable viremia, and increased blood markers of liver damage. Animals challenged by aerosol exhibited viremia and increased liver damage biomarkers with minimal observed clinical disease. All animals survived CHIKV challenge. CONCLUSIONS:We have described CHIKV infection in CMs following ID inoculation and, for the first time, infection by aerosol. Based on limited reported cases in the published literature, the aerosol model recapitulates the virologic findings of human infection via this route. The results of this study provide additional evidence for the potential use of CMs as a model for evaluating medical countermeasures against CHIKV.

journal_name

Virol J

journal_title

Virology journal

authors

Cirimotich CM,Vela EM,Garver J,Barnewall RE,Miller BD,Meister GT,Rogers JV

doi

10.1186/s12985-017-0804-7

subject

Has Abstract

pub_date

2017-07-20 00:00:00

pages

135

issue

1

issn

1743-422X

pii

10.1186/s12985-017-0804-7

journal_volume

14

pub_type

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