Abstract:
:The I5L gene is one of approximately 90 genes that are conserved throughout the chordopoxvirus family, and hence are presumed to play vital roles in the poxvirus life cycle. Previous work had indicated that the VP13 protein, a component of the virion membrane, was encoded by the I5L gene, but no additional studies had been reported. Using a recombinant virus that encodes an I5 protein fused to a V5 epitope tag at the endogenous locus (vI5V5), we show here that the I5 protein is expressed as a post-replicative gene and that the approximately 9 kDa protein does not appear to be phosphorylated in vivo. I5 does not appear to traffic to any cellular organelle, but ultrastructural and biochemical analyses indicate that I5 is associated with the membranous components of assembling and mature virions. Intact virions can be labeled with anti-V5 antibody as assessed by immunoelectron microscopy, indicating that the C' terminus of the protein is exposed on the virion surface. Using a recombinant virus which encodes only a TET-regulated copy of the I5V5 gene (vDeltaindI5V5), or one in which the I5 locus has been deleted (vDeltaI5), we also show that I5 is dispensable for replication in tissue culture. Neither plaque size nor the viral yield produced in BSC40 cells or primary human fibroblasts are affected by the absence of I5 expression.
journal_name
Virol Jjournal_title
Virology journalauthors
Unger B,Nichols RJ,Stanitsa ES,Traktman Pdoi
10.1186/1743-422X-5-148subject
Has Abstractpub_date
2008-12-15 00:00:00pages
148issn
1743-422Xpii
1743-422X-5-148journal_volume
5pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Acute gastroenteritis caused by bacteria, virus and parasite is an important cause of childhood morbidity and mortality in developing countries. Rotavirus and norovirus have been recognized as the most common pathogens causing acute gastroenteritis among children. However, there is still no valuable data abo...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-302
更新日期:2013-10-08 00:00:00
abstract:BACKGROUND:HCV is prevalent throughout the world. It is a major cause of chronic liver disease. There is no effective vaccine and the most common therapy, based on Peginterferon, has a success rate of ~50%. The mechanisms underlying viral resistance have not been elucidated but it has been suggested that both host and ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-14
更新日期:2012-01-12 00:00:00
abstract:BACKGROUND:Aedes aegypti is a competent arthropod vector of chikungunya virus (CHIKV). The rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. This stu...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0566-7
更新日期:2016-06-29 00:00:00
abstract:BACKGROUND:Crimean-Congo Hemorrhagic Fever virus (CCHFV), a member of the genus Nairovirus, family Bunyaviridae, is a tick-borne pathogen causing severe disease in humans. To better understand the CCHFV life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-42
更新日期:2005-04-25 00:00:00
abstract:BACKGROUND:SEN virus is a blood-borne, circular ssDNA virus and possessing nine genotypes (A to I). Among nine genotypes, SENV-D and SENV-H genotypes have the strong link with patients with unknown (none-A to E) hepatitis infections. Infection with blood-borne viruses is the second important cause of death in thalassem...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-1
更新日期:2010-01-02 00:00:00
abstract:BACKGROUND:Sequence and structural elements in the 3'-untranslated region (UTR) of Japanese encephalitis virus (JEV) are known to regulate translation and replication. We previously reported an abundant accumulation of small subgenomic flaviviral RNA (sfRNA) which is collinear with the highly conserved regions of the 3...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-492
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Controversy remains as to whether antiviral agents contribute to renal dysfunction in patients with chronic hepatitis B virus (HBV) infection. Thus, the aim of study was to analyze the changes in renal function of chronic hepatitis B (CHB) patients in response to anti-HBV therapy and the association with tre...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0712-x
更新日期:2017-03-09 00:00:00
abstract:BACKGROUND:The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and ca...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-75
更新日期:2012-03-27 00:00:00
abstract:BACKGROUND:A novel human enterovirus (HEV) type within the species HEV-C, named EV109, was discovered from cases of respiratory illness in Nicaragua in September 2010. The aim of this study, was to retrospectively examine the presence and the role of EV109 in respiratory samples from two patients populations; infants b...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-183
更新日期:2012-09-04 00:00:00
abstract:BACKGROUND:Human enterovirus (EV) and parechovirus (HPeV) are significant causes of encephalitis and meningitis in children. The aim of this study was to determine the prevalence, type and viral RNA concentration of EV and HPeV in cerebrospinal fluid (CSF) samples in an unselected cohort of patients <18 years admitted ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0427-9
更新日期:2015-11-25 00:00:00
abstract:BACKGROUND:Chikungunya virus (CHIKV) is transmitted via mosquito bite and potentially by aerosol, causing chikungunya fever and arthritic disease in humans. There are currently no licensed vaccines or antiviral therapeutics to protect against CHIKV infection in humans. Animal models recapitulating human disease, especi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0804-7
更新日期:2017-07-20 00:00:00
abstract:BACKGROUND:Classical swine fever (CSF) is one of the most important viral diseases of pigs. Clinical signs may vary from almost inapparent infection to a hemorrhagic fever like illness. Among the host factors leading to different disease courses are age, breed, and immune status. The aim of this study was to compare ho...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-134
更新日期:2014-07-29 00:00:00
abstract::Earlier, we detected viral RNAs packaged in the porcine adenovirus (PAdV) -3 virions. Using Southern blot analysis, we further demonstrated that the viral RNAs were predominantly packaged in CsCl purified mature capsids (containing viral genome) than empty/intermediate capsids. Some of the packaged viral RNAs appear t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-16
更新日期:2009-02-05 00:00:00
abstract:BACKGROUND:Torquetenovirus (TTV) belongs to Anelloviridae family, infects nearly all people indefinitely without causing overt disease establishing a fine and successful interaction with the host. Increasing evidence have shown some human viruses exploit extracellular vesicles thereby helping viral persistence in the h...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1055-y
更新日期:2018-09-20 00:00:00
abstract:BACKGROUND:Myxoma virus is a member of the Poxviridae and causes disease in European rabbits. Laboratory confirmation of the clinical disease, which occurs in the autumn of most years in Denmark, has been achieved previously using antigen ELISA and electron microscopy. RESULTS:An unusually large number of clinically s...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-7
更新日期:2010-01-16 00:00:00
abstract::Flaviviruses are small viruses with single-stranded RNA, which include the yellow fever virus, dengue virus, West Nile virus, Japanese encephalitis virus, tick-borne encephalitis virus, and Zika virus; and are causal agents of the most important emerging diseases that have no available treatment to date. In recent yea...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-017-0761-1
更新日期:2017-05-15 00:00:00
abstract:BACKGROUND:Herpesviruses are ubiquitous pathogens that infect and cause recurrent disease in multiple animal species. Feline herpesvirus-1 (FHV-1), a member of the alphaherpesvirus family, causes respiratory illness and conjunctivitis, and approximately 80% of domestic cats are latently infected. Oral administration of...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-34
更新日期:2014-02-22 00:00:00
abstract::Six major Hepatitis C virus (HCV) genotypes and hundreds of subtypes have been identified globally. All these genotypes are generally studied for epidemiology, their vaccine development and clinical management. This article comments the frequency distribution of various HCV genotypes circulate in different areas/distr...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-203
更新日期:2010-08-26 00:00:00
abstract:BACKGROUND:Dengue viruses (DENV) are the most important arboviruses of humans and cause significant disease. Infection with DENV elicits antibody responses to the envelope glycoprotein, predominantly against immunodominant, cross-reactive, weakly-neutralizing epitopes. These weakly-neutralizing antibodies are implicate...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-115
更新日期:2012-06-18 00:00:00
abstract:BACKGROUND:Tomato yellow leaf curl virus (TYLCV), a monopartite begomovirus (family Geminiviridae) is responsible for heavy yield losses for tomato production around the globe. In Oman at least five distinct begomoviruses cause disease in tomato, including TYLCV. Unusually, TYLCV infections in Oman are sometimes associ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0263-y
更新日期:2015-03-04 00:00:00
abstract:BACKGROUND:Host serine proteases are essential for the influenza virus life cycle because the viral haemagglutinin is synthesized as a precursor which requires proteolytic maturation. Therefore, we studied the activity and expression of serine proteases in lungs from mice infected with influenza and evaluated the effec...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-27
更新日期:2011-01-20 00:00:00
abstract:BACKGROUND:Highly pathogenic avian influenza virus (AIV) subtype H5N1 remains a threat to poultry. Duck enteritis virus (DEV)-vectored vaccines expressing AIV H5N1 hemagglutinin (HA) may be viable AIV and DEV vaccine candidates. METHODS:To facilitate the generation and further improvement of DEV-vectored HA(H5) vaccin...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0354-9
更新日期:2015-08-13 00:00:00
abstract::Avian influenza virus H9N2 isolates cause a mild influenza-like illness in humans. However, the pathogenesis of the H9N2 subtypes in human remains to be investigated. Using a human alveolar epithelial cell line A549 as host, we found that A/Quail/Hong Kong/G1/97 (H9N2/G1), which shares 6 viral "internal genes" with th...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-71
更新日期:2010-03-25 00:00:00
abstract:BACKGROUND:To understand the prevalence and characteristics of human cytomegalovirus (HCMV) infection in children in the Weifang area, and to provide information for its prevention and treatment. METHODS:A comprehensive survey was performed from 2009 to 2012 in 7582 children from birth to 6 years of age hospitalized i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-42
更新日期:2013-02-01 00:00:00
abstract:BACKGROUND:Classical swine fever virus (CSFV) is the member of the genus Pestivirus under the family Flaviviridae. The 5' untranslated region (UTR) of CSFV contains the IRES, which is a highly structured element that recruits the translation machinery. The 3' UTR is usually the recognition site of the viral replicase t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-340
更新日期:2011-07-06 00:00:00
abstract::Information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. Here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (HFV), of the Arenaviridae, Filoviridae, Bunyaviridae, and Flavivridae virus familie...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-81
更新日期:2011-02-24 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV) causes significant morbidity and mortality globally primarily due to its ability to cause hepatitis, liver cirrhosis and hepatocellular carcinoma. The Kenya National Blood Transfusion Services screens for Hepatitis B antibodies using the chemiluminescent microparticle immunoassay meth...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01447-2
更新日期:2020-11-13 00:00:00
abstract:BACKGROUND:The incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for dengue fever, and licensed vaccine against dengue is not available. ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-333
更新日期:2011-06-30 00:00:00
abstract:BACKGROUND:Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a highly contagious disease of birds and has been one of the major causes of economic losses in the poultry industry. Despite routine vaccination programs, sporadic cases have occasionally occurred in the country and remain a constant threat...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-183
更新日期:2010-08-08 00:00:00
abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01353-7
更新日期:2020-06-22 00:00:00