当前位置： SCI文献检索 > Viruses-Basel期刊下所有文献 > Ubiquitin and Not Only Unfolded Domains Drives Toscana Virus Non-Structural NSs Protein Degradation.

Ubiquitin and Not Only Unfolded Domains Drives Toscana Virus Non-Structural NSs Protein Degradation.

Abstract：

:The non-structural protein NSs of the Phenuiviridae family members appears to have a role in the host immunity escape. The stability of Toscana virus (TOSV) NSs protein was tested by a cycloheximide (CHX) chase approach on cells transfected with NSs deleted versions fused to a reporter gene. The presence of intrinsically disordered regions (IDRs) both at the C- and N-terminus appeared to affect the protein stability. Indeed, the NSsΔC and NSsΔN proteins were more stable than the wild-type NSs counterpart. Since TOSV NSs exerts its inhibitory function by triggering RIG-I for proteasomal degradation, the interaction of the ubiquitin system and TOSV NSs was further examined. Chase experiments with CHX and the proteasome inhibitor MG-132 demonstrated the involvement of the ubiquitin-proteasome system in controlling NSs protein amount expressed in the cells. The analysis of TOSV NSs by mass spectrometry allowed the direct identification of K104, K109, K154, K180, K244, K294, and K298 residues targeted for ubiquitination. Analysis of NSs K-mutants confirmed the presence and the important role of lysine residues located in the central and the C-terminal parts of the protein in controlling the NSs cellular level. Therefore, we directly demonstrated a new cellular pathway involved in controlling TOSV NSs fate and activity, and this opens the way to new investigations among more pathogenic viruses of the Phenuiviridae family.

journal_name

Viruses

journal_title

Viruses

authors

Gori Savellini G,Bini L,Gagliardi A,Anichini G,Gandolfo C,Prathyumnan S,Cusi MG

doi

10.3390/v12101153

subject

Has Abstract

pub_date

2020-10-12 00:00:00

issue

10

issn

1999-4915

pii

v12101153

journal_volume

12

pub_type

杂志文章

相关文献

Viruses-Basel文献大全
  • BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape.

    abstract：:Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with diffe...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v7082815

    authors： Chen Q,Wang J,Hou M,Liu S,Huang J,Cai L

    更新日期：2015-07-27 00:00:00

  • Distinct Contributions of Autophagy Receptors in Measles Virus Replication.

    abstract：:Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order to target them towards autophagy for their degradation after the fusion of pathogen-containing autophagosomes with lysosomes...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v9050123

    authors： Petkova DS,Verlhac P,Rozières A,Baguet J,Claviere M,Kretz-Remy C,Mahieux R,Viret C,Faure M

    更新日期：2017-05-22 00:00:00

  • Surf and turf: mechanism of enhanced virus spread during poxvirus infection.

    abstract：:Commentary on Doceul, V.; Hollinshead, M.; van der Linden, L.; Smith, G.L. Repulsion of superinfecting virions: a mechanism for rapid virus spread. Science2010, 327, 873-876. ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v2051050

    authors： Condit RC

    更新日期：2010-05-01 00:00:00

  • High Throughput Screening of FDA-Approved Drug Library Reveals the Compounds that Promote IRF3-Mediated Pro-Apoptotic Pathway Inhibit Virus Replication.

    abstract：:Interferon (IFN) regulatory factor 3 (IRF3) is the key transcription factor for the induction of IFN and antiviral genes. The absence of antiviral genes in IRF3 deficiency leads to susceptibility to a wide range of viral infections. Previously, we uncovered a function for nontranscriptional IRF3 (nt-IRF3), RLR (RIG-I-...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12040442

    authors： Glanz A,Chawla K,Fabry S,Subramanian G,Garcia J,Jay B,Ciricillo J,Chakravarti R,Taylor RT,Chattopadhyay S

    更新日期：2020-04-14 00:00:00

  • Comment: Mis-Genotyping of Some Hepatitis D Virus Genotype 2 and 5 Sequences Using HDVdb.

    abstract：:Evidence that Hepatitis D virus (HDV) genotype is involved in HDV infection pathogenesis is increasing. Indeed, HDV genotypes have been shown to be linked to different outcomes in terms of liver fibrosis and treatment response. Herein, we show that the promising HDVdb genotyping tool available online can lead to wrong...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12101066

    authors： Charre C,le Gal F,Dény P,Scholtès C

    更新日期：2020-09-23 00:00:00

  • Characterization of Nucleoside Reverse Transcriptase Inhibitor-Associated Mutations in the RNase H Region of HIV-1 Subtype C Infected Individuals.

    abstract：:The South African national treatment programme includes nucleoside reverse transcriptase inhibitors (NRTIs) in both first and second line highly active antiretroviral therapy regimens. Mutations in the RNase H domain have been associated with resistance to NRTIs but primarily in HIV-1 subtype B studies. Here, we inves...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v9110330

    authors： Ngcapu S,Theys K,Libin P,Marconi VC,Sunpath H,Ndung'u T,Gordon ML

    更新日期：2017-11-08 00:00:00

  • Prognostic Factors of Severe Fever with Thrombocytopenia Syndrome in South Korea.

    abstract：:Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne infectious disease, is difficult to differentiate from other common febrile diseases. Clinically distinctive features and climate variates associated with tick growth can be useful predictors for SFTS. This retrospective study (2013-2019) demonstrated th...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v13010010

    authors： Kim M,Heo ST,Oh H,Oh S,Lee KH,Yoo JR

    更新日期：2020-12-23 00:00:00

  • Updating the Quarantine Status of Prunus Infecting Viruses in Australia.

    abstract：:One hundred Prunus trees, including almond (P. dulcis), apricot (P. armeniaca), nectarine (P. persica var. nucipersica), peach (P. persica), plum (P. domestica), purple leaf plum (P. cerasifera) and sweet cherry (P. avium), were selected from growing regions Australia-wide and tested for the presence of 34 viruses and...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12020246

    authors： Kinoti WM,Nancarrow N,Dann A,Rodoni BC,Constable FE

    更新日期：2020-02-23 00:00:00

  • Incomplete LPS Core-Specific Felix01-Like Virus vB_EcoM_VpaE1.

    abstract：:Bacteriophages represent a valuable source for studying the mechanisms underlying virus-host interactions. A better understanding of the host-specificity of viruses at the molecular level can promote various phage applications, including bacterial diagnostics, antimicrobial therapeutics, and improve methods in molecul...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v7122932

    authors： Šimoliūnas E,Vilkaitytė M,Kaliniene L,Zajančkauskaitė A,Kaupinis A,Staniulis J,Valius M,Meškys R,Truncaitė L

    更新日期：2015-11-27 00:00:00

  • Interaction of a Densovirus with Glycans of the Peritrophic Matrix Mediates Oral Infection of the Lepidopteran Pest Spodoptera frugiperda.

    abstract：:The success of oral infection by viruses depends on their capacity to overcome the gut epithelial barrier of their host to crossing over apical, mucous extracellular matrices. As orally transmitted viruses, densoviruses, are also challenged by the complexity of the insect gut barriers, more specifically by the chitino...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11090870

    authors： Pigeyre L,Schatz M,Ravallec M,Gasmi L,Nègre N,Clouet C,Seveno M,El Koulali K,Decourcelle M,Guerardel Y,Cot D,Dupressoir T,Gosselin-Grenet AS,Ogliastro M

    更新日期：2019-09-17 00:00:00

  • Hepatitis E Pathogenesis.

    abstract：:Although most hepatitis E virus (HEV) infections are asymptomatic, some can be severe, causing fulminant hepatitis and extra-hepatic manifestations, including neurological and kidney injuries. Chronic HEV infections may also occur in immunocompromised patients. This review describes how our understanding of the pathog...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v8080212

    authors： Lhomme S,Marion O,Abravanel F,Chapuy-Regaud S,Kamar N,Izopet J

    更新日期：2016-08-05 00:00:00

  • Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4).

    abstract：:Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer. Controlled dissociation of virions provides important information on virion composition, interactions, and stoichiometry of virion components, as well as their...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10110579

    authors： Eskelin K,Poranen MM

    更新日期：2018-10-23 00:00:00

  • Duck Plague Virus Promotes DEF Cell Apoptosis by Activating Caspases, Increasing Intracellular ROS Levels and Inducing Cell Cycle S-Phase Arrest.

    abstract：BACKGROUND:Duck plague virus (DPV) can induce apoptosis in duck embryo fibroblasts (DEFs) and in infected ducks, but the molecular mechanism of DPV-induced apoptosis remains unknown. METHODS:We first used qRT-PCR and a Caspase-Glo assay to determine whether the caspase protein family plays an important role in DPV-ind...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11020196

    authors： Zhao C,Wang M,Cheng A,Yang Q,Wu Y,Jia R,Zhu D,Chen S,Liu M,Zhao X,Zhang S,Liu Y,Yu Y,Zhang L,Tian B,Rehman MU,Pan L,Chen X

    更新日期：2019-02-24 00:00:00

  • A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation.

    abstract：:The objective of this study was to evaluate the effects of A3R phage and Staphylococcus aureus lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expressio...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v9020036

    authors： Borysowski J,Międzybrodzki R,Wierzbicki P,Kłosowska D,Korczak-Kowalska G,Weber-Dąbrowska B,Górski A

    更新日期：2017-02-21 00:00:00

  • Virology, Epidemiology, Pathogenesis, and Control of COVID-19.

    abstract：:The outbreak of emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) in China has been brought to global attention and declared a pandemic by the World Health Organization (WHO) on March 11, 2020. Scientific advancements since the pandemic of severe acute respiratory syndrome (SARS)...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v12040372

    authors： Jin Y,Yang H,Ji W,Wu W,Chen S,Zhang W,Duan G

    更新日期：2020-03-27 00:00:00

  • Structural and Functional Characterization of the Secondary Mutation N126K Selected by Various HIV-1 Fusion Inhibitors.

    abstract：:Peptides derived from the C-terminal heptad repeat (CHR) region of HIV-1 gp41 is potent viral membrane fusion inhibitors, such as the first clinically approved peptide drug T20 and a group of newly-designed peptides. The resistance profiles of various HIV-1 fusion inhibitors were previously characterized, and the seco...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v12030326

    authors： Yu D,Su Y,Ding X,Zhu Y,Qin B,Chong H,Cui S,He Y

    更新日期：2020-03-18 00:00:00

  • Attenuated Influenza Virions Expressing the SARS-CoV-2 Receptor-Binding Domain Induce Neutralizing Antibodies in Mice.

    abstract：:An effective vaccine is essential for controlling the spread of the SARS-CoV-2 virus. Here, we describe an influenza virus-based vaccine for SARS-CoV-2. We incorporated a membrane-anchored form of the SARS-CoV-2 spike receptor binding domain (RBD) in place of the neuraminidase (NA) coding sequence in an influenza viru...

    journal_title：Viruses

    pub_type： 评论,杂志文章

    doi：10.3390/v12090987

    authors： Loes AN,Gentles LE,Greaney AJ,Crawford KHD,Bloom JD

    更新日期：2020-09-05 00:00:00

  • Heat Shock Enhances the Expression of the Human T Cell Leukemia Virus Type-I (HTLV-I) Trans-Activator (Tax) Antigen in Human HTLV-I Infected Primary and Cultured T Cells.

    abstract：:The environmental factors that lead to the reactivation of human T cell leukemia virus type-1 (HTLV-I) in latently infected T cells in vivo remain unknown. It has been previously shown that heat shock (HS) is a potent inducer of HTLV-I viral protein expression in long-term cultured cell lines. However, the precise HTL...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v8070191

    authors： Kunihiro M,Fujii H,Miyagi T,Takahashi Y,Tanaka R,Fukushima T,Ansari AA,Tanaka Y

    更新日期：2016-07-11 00:00:00

  • Exploratory Spatial Analysis of in vitro Respiratory Syncytial Virus Co-infections.

    abstract：:The cell response to virus infection and virus perturbation of that response is dynamic and is reflected by changes in cell susceptibility to infection. In this study, we evaluated the response of human epithelial cells to sequential infections with human respiratory syncytial virus strains A2 and B to determine if a ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v2122782

    authors： Simeonov I,Gong X,Kim O,Poss M,Chiaromonte F,Fricks J

    更新日期：2010-12-01 00:00:00

  • Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells.

    abstract：:The mouse mammary tumor virus (MMTV) Pr77Gag polypeptide is an essential retroviral structural protein without which infectious viral particles cannot be formed. This process requires specific recognition and packaging of dimerized genomic RNA (gRNA) by Gag during virus assembly. Most of the previous work on retrovira...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10060334

    authors： Chameettachal A,Pillai VN,Ali LM,Pitchai FNN,Ardah MT,Mustafa F,Marquet R,Rizvi TA

    更新日期：2018-06-18 00:00:00

  • Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level during ART.

    abstract：:Depletion and functional impairment of circulating plasmacytoid dendritic cells (pDCs) are characteristic attributes of HIV-1-infection. The mechanism of dysfunction of pDCs is unclear. Here, we studied the development of phenotype of pDCs in a cohort of HIV-1-infected individuals monitored before the initiation and d...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10040154

    authors： Font-Haro A,Janovec V,Hofman T,Machala L,Jilich D,Melkova Z,Weber J,Trejbalova K,Hirsch I

    更新日期：2018-03-28 00:00:00

  • Adaption of FMDV Asia-1 to Suspension Culture: Cell Resistance Is Overcome by Virus Capsid Alterations.

    abstract：:Foot-and-mouth disease virus (FMDV) causes a highly contagious disease with catastrophic economic impact for affected countries. BHK21 suspension cells are preferred for the industrial production of FMDV vaccine antigen, but not all virus strains can be successfully propagated in these cells. Serotype Asia-1 is often ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v9080231

    authors： Dill V,Hoffmann B,Zimmer A,Beer M,Eschbaumer M

    更新日期：2017-08-18 00:00:00

  • New Markers in Monitoring the Reactivation of Hepatitis B Virus Infection in Immunocompromised Hosts.

    abstract：:Hepatitis B virus (HBV) persistence is at the basis of HBV reactivation as a consequence of chemotherapy and immunosuppressive treatments. The identification of early viral replication indicators and markers of effective HBV immunological control would be useful in monitoring patients who are at risk of potential vira...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v11090783

    authors： Svicher V,Salpini R,Malagnino V,Piermatteo L,Alkhatib M,Cerva C,Sarmati L

    更新日期：2019-08-25 00:00:00

  • Recombinant GII.P16/GII.4 Sydney 2012 Was the Dominant Norovirus Identified in Australia and New Zealand in 2017.

    abstract：:For the past two decades, norovirus pandemic variants have emerged every 3⁻5 years, and dominate until they are replaced by alternate strains. However, this scenario changed in 2016 with the co-circulation of six prevalent viruses, three of which possessed the pandemic GII.4 Sydney 2012 capsid. An increased number of ...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v10100548

    authors： Lun JH,Hewitt J,Yan GJH,Enosi Tuipulotu D,Rawlinson WD,White PA

    更新日期：2018-10-09 00:00:00

  • Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity.

    abstract：:Abstract: Since 2007, highly pathogenic clade 2.3.2 H5N1 avian influenza A (A(H5N1)) viruses have evolved to clade 2.3.2.1a, b, and c; currently only 2.3.2.1c A(H5N1) viruses circulate in wild birds and poultry. During antigenic evolution, clade 2.3.2.1a and c A(H5N1) viruses acquired both S144N and V223I mutations ar...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v11100923

    authors： An SH,Lee CY,Hong SM,Song CS,Kim JH,Kwon HJ

    更新日期：2019-10-09 00:00:00

  • Resistance to HIV Integrase Inhibitors: About R263K and E157Q Mutations.

    abstract：:The use of integrase inhibitors (INI) is increasing in antiretroviral therapies (ART) and INI are not all equal regarding genetic barrier to resistance. The aim of this manuscript was to review main in vivo and in vitro knowledge about two particular integrase resistance-associated mutations: R263K and E157Q. The R263...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v10010041

    authors： Charpentier C,Descamps D

    更新日期：2018-01-18 00:00:00

  • The cyclin-dependent kinase ortholog pUL97 of human cytomegalovirus interacts with cyclins.

    abstract：:The human cytomegalovirus (HCMV)-encoded protein kinase, pUL97, is considered a cyclin-dependent kinase (CDK) ortholog, due to shared structural and functional characteristics. The primary mechanism of CDK activation is binding to corresponding cyclins, including cyclin T1, which is the usual regulatory cofactor of CD...

    journal_title：Viruses

    pub_type： 杂志文章

    doi：10.3390/v5123213

    authors： Graf L,Webel R,Wagner S,Hamilton ST,Rawlinson WD,Sticht H,Marschall M

    更新日期：2013-12-18 00:00:00

  • Vaccinia Virus Natural Infections in Brazil: The Good, the Bad, and the Ugly.

    abstract：:The orthopoxviruses (OPV) comprise several emerging viruses with great importance to human and veterinary medicine, including vaccinia virus (VACV), which causes outbreaks of bovine vaccinia (BV) in South America. Historically, VACV is the most comprehensively studied virus, however, its origin and natural hosts remai...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v9110340

    authors： Oliveira JS,Figueiredo PO,Costa GB,Assis FL,Drumond BP,da Fonseca FG,Nogueira ML,Kroon EG,Trindade GS

    更新日期：2017-11-15 00:00:00

  • Brockman, M.A., et al., Human Leukocyte Antigen (HLA) Class I Down-Regulation by Human Immunodeficiency Virus Type 1 Negative Factor (HIV-1 Nef): What Might We Learn From Natural Sequence Variants? Viruses 2012, 4, 1711-1730.

    abstract：:In the original manuscript, the text in figure 1 is illegible. Furthermore, there is an unnecessary carriage return (page 1716, ~line 18) "crystallographic ... methods". ...

    journal_title：Viruses

    pub_type： 杂志文章,已发布勘误

    doi：10.3390/v4102014

    authors： Mwimanzi P,Markle TJ,Ueno T,Brockman MA

    更新日期：2012-10-05 00:00:00

  • The molecular biology of feline immunodeficiency virus (FIV).

    abstract：:Feline immunodeficiency virus (FIV) is widespread in feline populations and causes an AIDS-like illness in domestic cats. It is highly prevalent in several endangered feline species. In domestic cats FIV infection is a valuable small animal model for HIV infection. In recent years there has been sa significant increas...

    journal_title：Viruses

    pub_type： 杂志文章,评审

    doi：10.3390/v3112192

    authors： Kenyon JC,Lever AM

    更新日期：2011-11-01 00:00:00